A5086473420- Photoreceptor and optogenetics research
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Molecular Communication and Nanonetworks
- Neuroscience and Neuropharmacology Research
- Crystallography and molecular interactions
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Mass Spectrometry Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Plant and Biological Electrophysiology Studies
- Pharmacological Effects and Assays
- Advanced Battery Technologies Research
- Meat and Animal Product Quality
- Zebrafish Biomedical Research Applications
- Electrochemical Analysis and Applications
- Stroke Rehabilitation and Recovery
- Acute Ischemic Stroke Management
- Veterinary Equine Medical Research
- Neurological disorders and treatments
- bioluminescence and chemiluminescence research
- Advanced Fluorescence Microscopy Techniques
- Parkinson's Disease Mechanisms and Treatments
Humboldt-Universität zu Berlin
2013-2024
Charité - Universitätsmedizin Berlin
2022-2024
Freie Universität Berlin
2022-2024
University of Göttingen
2019
The field of optogenetics uses channelrhodopsins (ChRs) for light-induced neuronal activation. However, optimized tools cellular inhibition at moderate light levels are lacking. We found that replacement E90 in the central gate ChR with positively charged residues produces chloride-conducting ChRs (ChloCs) only negligible cation conductance. Molecular dynamics modeling unveiled a high-affinity Cl(-)-binding site had been generated near gate. Stabilizing open state dramatically increased...
A new microbial rhodopsin class that actively transports sodium out of the cell upon illumination was described in 2013. However, poor membrane targeting first-identified pump KR2 mammalian cells has hindered direct electrical investigation its transport mechanism and optogenetic application to date. Accordingly, we designed enhanced (eKR2), which exhibits improved higher photocurrents facilitate molecular characterization future applications. Our selectivity measurements revealed stationary...
The extracellular ionic environment in neural tissue has the capacity to influence, and be influenced by, natural bouts of activity. We employed optogenetic approaches control investigate these interactions within between cells, across spatial scales. began by developing a temporally precise means study microdomain-scale protons acid-sensing ion channels (ASICs). By coupling single-component proton-transporting tools ASICs create two-component constructs (TCOs), we found that acidification...
Parkinson's Disease (PD) is characterized by complex motor deficits, including transient akinetic episodes during locomotion. Here, we investigated the neural correlates of akinesia in a 6-OHDA rat model PD. We developed open-source toolbox neurokin to analyse kinematic and signal recorded runway task. First, observed that compared control, PD rats spent significantly more time episodes, which were correlated with an increase beta-band power. Next, computed set temporally resolved features...
Interest in microbial rhodopsins with ion pumping activity has been revitalized the context of optogenetics, where light-driven pumps are used for cell hyperpolarization and voltage sensing. We identified an opsin-encoding gene (CsR) genome arctic alga Coccomyxa subellipsoidea C-169 that can produce large photocurrents Xenopus oocytes. this property to analyze function individual residues proton pumping. Modification highly conserved shuttling residue R83 or its interaction partner Y57...
Structural analysis enables conversion of an Arctic algal light-driven ion pump into a light-gated channel.
Sensory photoreceptors abound in nature and enable organisms to adapt behavior, development, physiology environmental light. In optogenetics, allow spatiotemporally precise, reversible, non-invasive control by light of cellular processes. Notwithstanding the development numerous optogenetic circuits, an unmet demand exists for efficient circuits sensitive red light, given its superior penetration biological tissue. Bacteriophytochrome sense ratio far-red regulate activity enzymatic effector...
Accurate assessment of post-stroke deficits is crucial in translational research. Recent advances machine learning offer precise quantification rodent motor behavior post-stroke, yet detecting lesion-specific upper extremity remains unclear. Employing proximal middle cerebral artery occlusion (MCAO) and cortical photothrombosis (PT) mice, we assessed impairments via the Staircase test. Lesion locations were identified using 7 T-MRI. Machine was applied to reconstruct forepaw kinematic...
Abstract The biophysical characterization and engineering of optogenetic tools photobiological systems has been hampered by the lack efficient methods for spectral illumination microplates high-throughput analysis action spectra. Current to determine spectra only allow sequential individual wells. Here we present open-source RainbowCap-system, which combines LEDs optical filters in a standard 96-well microplate format simultaneous spectrally defined illumination. RainbowCap provides equal...
Sensory photoreceptors abound in nature and enable organisms to adapt behavior, development, physiology environmental light. In optogenetics, allow spatiotemporally precise, reversible, non-invasive control by light of cellular processes. Notwithstanding the development numerous optogenetic circuits, an unmet demand exists for efficient circuits sensitive red light, given its superior penetration biological tissue. Bacteriophytochrome sense ratio far-red regulate activity enzymatic effector...
Abstract Accurate assessment of post-stroke deficits is vital in translational research. Recent advances machine learning provide unprecedented precision quantifying rodent motor behavior post-stroke. However, the extent to which these tools can detect lesion-specific upper extremity remains unclear. Using proximal middle cerebral artery occlusion (MCAO) and cortical photothrombosis (PT), we assessed impairments mice through Staircase test. Lesion locations were identified using 7T-MRI....