A5102715155- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Sperm and Testicular Function
- Renal and related cancers
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Advanced biosensing and bioanalysis techniques
MRC Centre for Regenerative Medicine
2018-2021
University of Edinburgh
2018-2021
European Molecular Biology Laboratory
2013-2019
Abstract Several developmental stages of spermatogenesis are transcriptionally quiescent which presents major challenges associated with the regulation gene expression. Here we identify that zygotene to pachytene transition is not only resumption transcription but also a wave programmed mRNA degradation essential for meiotic progression. We explored whether terminal uridydyl transferase 4- (TUT4-) or TUT7-mediated 3′ uridylation contributes this during pachynema. Indeed, both TUT4 and TUT7...
Abstract The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. piRNAs are proposed to recruit transcriptionally TE loci by base pairing nascent transcripts, however downstream mechanisms effector proteins utilized directing de novo remain incompletely understood. Here, we show that associates with TEX15 foetal gonocytes. is predominantly a nuclear not required for piRNA biogenesis...
Spermatogonial stem cells (SSCs) sustain spermatogenesis and fertility throughout adult male life. The conserved RNA-binding protein NANOS2 is essential for the maintenance of SSCs, but its targets mechanisms function are not fully understood. Here, we generated a functional epitope-tagged Nanos2 mouse allele applied highly stringent cross-linking analysis cDNAs to define RNA occupancy in SSC lines. recognizes AUKAAWU consensus motif, mostly found 3′ untranslated region defined messenger...