A5059108235- Pancreatic function and diabetes
- Diabetes Management and Research
- Diabetes Treatment and Management
- Diabetes and associated disorders
- Ion Channels and Receptors
- Phagocytosis and Immune Regulation
- Congenital Diaphragmatic Hernia Studies
- Medical Imaging and Pathology Studies
- Exercise and Physiological Responses
- Pediatric Urology and Nephrology Studies
- Drug Transport and Resistance Mechanisms
- Biochemical Analysis and Sensing Techniques
- Plant Stress Responses and Tolerance
- Menstrual Health and Disorders
- Metabolism, Diabetes, and Cancer
University of Alberta
2020-2022
Oxford Centre for Diabetes, Endocrinology and Metabolism
2016
Churchill Hospital
2016
University of Oxford
2016
John Radcliffe Hospital
2016
The University of Texas Health Science Center at San Antonio
1980
In diabetes, glucagon secretion from pancreatic α cells is dysregulated. The underlying mechanisms, and whether dysfunction occurs uniformly among cells, remain unclear. We examined human donors mice using electrophysiological, transcriptomic, computational approaches. Rising glucose suppresses cell exocytosis by reducing P/Q-type Ca2+ channel activity, this disrupted in type 2 diabetes (T2D). Upon high-fat feeding of mice, shift toward a "β cell-like" electrophysiological profile concert...
Our study shows that glucagon-like peptide-1 (GLP-1) is secreted within human islets and may play an unexpectedly important paracrine role in islet physiology pathophysiology. It known α cells rodent pancreatic are capable of secreting GLP-1, but little about the functional islet-derived GLP-1 plays islets.We used flow cytometry, immunohistochemistry, perifusions, calcium imaging techniques to analyse expression function isolated from cadaveric donors with or without type 2 diabetes. We also...
Clinical islet transplantation achieves insulin independence in selected patients, yet current methods for extracting islets from their surrounding pancreatic matrix are suboptimal. The basement membrane (BM) influences function and survival is a critical marker of integrity following rodent isolation. No studies have investigated the impact isolation on BM human islets, which unique duplex structure. To address this, samples were taken 27 clinical isolations (donor age 41-59, BMI 26-38,...
Background One of the goals clinical islet transplantation is to achieve a single-donor transplant that dependent on obtaining enough quality cell mass from one donor pancreas. Human islets are routinely cultured prior transplantation, and pro-survival factors such as GLP-1 analogues have been reported maintain survival. Interestingly, human may secrete they also express enzyme DPP4 proteolytically cleaves into an inactive form. The aim this study investigate secretion test if inhibitor...
TRPM5 is a calcium-activated monovalent cation channel that expressed in human and murine islets. Ion channels of the TRP family are multimodal sensors activated by different stimuli such as voltage, temperature ligands, including natural compounds. Previous work showed mice lacking have increased postprandial blood glucose levels an impaired insulin secretion. There observations mutations patients suffering from diabetes or metabolic syndrome. Stevioside stevia sweet-tasting organic...