A5029839843- Sperm and Testicular Function
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Cell Adhesion Molecules Research
- Plant Reproductive Biology
- Proteins in Food Systems
- Seed Germination and Physiology
- Lipid Membrane Structure and Behavior
- Reproductive biology and impacts on aquatic species
- Hormonal and reproductive studies
- Reproductive System and Pregnancy
- Fern and Epiphyte Biology
- Ovarian function and disorders
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
- Chemical Synthesis and Analysis
- Cancer-related gene regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Platelet Disorders and Treatments
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Cellular Mechanics and Interactions
- Connexins and lens biology
University of California, Davis
2001-2011
National Institute of Environmental Health Sciences
1998
National Institutes of Health
1998
UConn Health
1987-1997
University of Connecticut
1983-1995
University of California, San Francisco
1990-1995
University of Virginia
1995
The University of Adelaide
1993
Northwestern University
1993
Queen Elizabeth Hospital
1993
Fertilin, a member of the ADAM family, is found on plasma membrane mammalian sperm. Sperm from mice lacking fertilin β were shown to be deficient in sperm-egg adhesion, fusion, migration uterus into oviduct, and binding egg zona pellucida. Egg activation was unaffected. The results are consistent with direct role interaction. Fertilin could also have sperm-zona or oviduct migration; alternatively, effects these functions result absence activity during spermatogenesis.
A typical mammalian egg is surrounded by an outer layer of about 3,000 cumulus cells embedded in extracellular matrix rich hyaluronic acid. current, widely proposed model that the fertilizing sperm, while it acrosome intact, passes through cell and binds to zona pellucida. This current lacks a well-supported explanation for how sperm penetrate layer. We report protein PH-20 has hyaluronidase activity present on plasma membrane mouse human sperm. Brief treatment with purified, recombinant can...
After the acrosome reaction, PH-20 surface antigen of guinea pig sperm migrates from its original location on posterior head to a new inner acrosomal membrane (Myles, D.G., and P. Primakoff, 1984, J. Cell Biol., 99:1634-1641). We have isolated three monoclonal antibodies (MAbs) IgG1 subclass, PH-20, PH-21, PH-22, that bind antigen. The MAb strongly inhibited (approximately 90%) binding egg zona pellucida at saturating antibody concentrations (greater than 20 micrograms/ml). Half-maximal...
Fertilization and certain later stages in mammalian embryonic development require fusion between membranes of individual cells. The mechanism eukaryotic cell-cell is unknown, no surface molecules required for this process have been unequivocally identified. role the sperm protein fertilin sperm-egg was tested by using peptide analogues a potential integrin binding site beta subunit. Peptide that include TDE sequence from disintegrin region are able to bind egg plasma membrane strongly...
Sperm–egg plasma membrane fusion is preceded by sperm adhesion to the egg membrane. Cell–cell frequently involves multiple molecules on adhering cells. One surface protein with a role in sperm–egg fertilin, transmembrane heterodimer (α and β subunits). Fertilin α are first identified members of new family proteins that each has following domains: pro-, metalloprotease, disintegrin, cysteine-rich, EGF-like, transmembrane, cytoplasmic domain. This been named ADAM because all contain...
A protein located on the surface of guinea pig sperm (PH-30) has been implicated in process sperm-egg fusion (Primakoff, P., H. Hyatt, and J. Tredick-Kline. 1987. Cell Biol. 104:141-149). In this paper we have assessed basic biochemical properties PH-30 analyzed molecular forms present at different stages maturation. We show following: (a) is an integral membrane glycoprotein; (b) it composed two tightly associated immunologically distinct subunits; (c) both subunits are made as larger...
We have previously defined distinct localizations of antigens on the surface guinea pig sperm using monoclonal antibodies. In present study we demonstrated that these antigen are dynamic and can be altered during changes in functional state sperm. Before is capable fertilizing egg, it must undergo capacitation an exocytic event, acrosome reaction. Prior to capacitation, recognized by antibody, PT-1, was restricted posterior tail region (principle piece end piece). After incubation...
PH-30, a sperm surface protein involved in sperm-egg fusion, is composed of two subunits, alpha and beta, which are synthesized as precursors processed, during development, to yield the mature forms. The PH-30 alpha/beta complex resembles certain viral fusion proteins membrane topology predicted binding functions. Furthermore, subunits similar sequence each other family disintegrin domain-containing snake venom proteins. We report here sequences beta precursor regions. Their domain...
On terminally differentiated sperm cells, surface proteins are segregated into distinct domains that include the anterior and posterior head domains. We have analyzed formation of guinea pig in terms both timing protein localization mechanism(s) responsible. testicular sperm, PH-20, PH-30 AH-50 were found to be present on whole cell (PH-20) or (PH-30, AH-50). completed differentiation (cauda epididymal sperm), PH-20 restricted domain was domain. Thus these become their distribution late...
Abstract Izumo, a sperm membrane protein, is essential for gamete fusion in the mouse. It has an Immunoglobulin (Ig) domain and N‐terminal which neither functions nor homologous sequences are known. In present work we identified three novel proteins showing with significant homology to of Izumo. We named this region “Izumo domain,” 2,” 3,” 4,” retaining 1” first described member family. Izumo 1–3 transmembrane expressed specifically testis, 4 soluble protein testis other tissues....
Previous results, based on inhibition of fertilization by an anti–α6 integrin mAb (GoH3), suggest that the α6β1 mouse eggs functions as receptor for sperm (Almeida, E.A., A.P. Huovila, A.E. Sutherland, L.E. Stephens, P.G. Calarco, L.M. Shaw, A.M. Mercurio, A. Sonnenberg, P. Primakoff, D.G. Myles, and J.M. White. 1995. Cell. 81:1095–1104). Because egg surface tetraspanin CD9 is essential gamete fusion (Kaji, K., S. Oda, T. Shikano, Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, Miyazaki, Kudo....
Sperm binding to the egg zona pellucida in mammals is a cell-cell adhesion process that generally species specific. The guinea pig sperm protein PH-20 has required function of eggs. located on both plasma membrane and acrosomal membrane. We report here isolation sequence full-length cDNA for (available from EMBL/GenBank/DDBJ under accession number X56332). derived amino acid shows mature 468 acids containing six N-linked glycosylation sites twelve cysteines, eight which are tightly clustered...