The unicellular heterotrophic thraustochytrids are attractive candidates for commercial polyunsaturated fatty acids (PUFA) production. However, the reactive oxygen species (ROS) generated in their aerobic fermentation process often limits PUFA titer. Yet, specific mechanisms of ROS involvement crosstalk between oxidative stress and intracellular lipid synthesis remain poorly described. Metabolic engineering to improve yield without compromising growth is an important aspect economic...

Thraustochytrids, rich in docosahexaenoic acid (DHA, C22:6ω3), represent a potential source of dietary fatty acids. Yet, the effect culture conditions on growth and composition vary widely among different thraustochytrid strains. Two strains, Schizochytrium sp. PKU#Mn4 Thraustochytriidae PKU#Mn16 were studied for their DHA production characteristics under various conditions. Although they exhibited similar profiles, seemed good candidate industrial fermentation while displayed tolerance to...

Guided by miRNAs, RNAi plays an important role in virus-host interactions fine-tuning gene expression. Many viral and cellular miRNAs are involved virus infection, though no comprehensive general model for derived from invertebrate DNA viruses exists their function eukaryotic systems, despite extensive research on miRNAs. To address this issue, the shrimp white spot syndrome (WSSV), a with 305 kb double-stranded circular genome, were characterized. Based WSSV miRNA microarray northern blot...

ABSTRACT Viral microRNAs (miRNAs), most of which are characterized in cell lines, have been found to play important roles the virus life cycle avoid attack by host immune system or keep latency state. miRNAs targeting genes can inhibit infection. In this study, vivo findings Marsupenaeus japonicus shrimp revealed that viral could target and further promote The results showed white spot syndrome (WSSV)-encoded WSSV-miR-66 WSSV-miR-68 were transcribed at early stage WSSV When expression was...

Fungi play a critical role in the nutrient cycling and ecological function terrestrial freshwater ecosystems. Yet, many aspects of their counterparts coastal ecosystems remain largely elusive. Using high-throughput sequencing, quantitative PCR, environmental data analyses, we studied spatiotemporal changes abundance diversity planktonic fungi abiotic biotic interactions waters three transects along Bohai Sea. A total 4362 ITS OTUs were identified more than 60% which unclassified Fungi. Of...

This study reports comparative evaluation of the growth and DHA productivity thraustochytrid strain Thraustochytriidae PKU#Mn16 fermented with seven different substrate feeding strategies. Of these strategies, fed-batch fermentation mixed (glucose & glycerol) yielded maximum (52.2 ± 1.5 g/L), yield (Yp/s: 8.65) (100.7 2.9 mg/L-h), comparable those previously reported Aurantiochytrium strains. Transcriptomics analyses revealed that glucose upregulated some genes fatty acid synthase pathway...

Qinhuangdao coastal waters in northern China are heavily impacted by anthropogenic and natural activities, we anticipate a direct influence of the impact on bacterioplankton abundance diversity inhabiting adjacent areas. To ascertain influences, first evaluated seasonal patterns areas with varied levels activities then analyzed environmental factors which influenced patterns. Results indicated distinct across warm cold seasons all stations. Total bacterial stations ranged from 8.67 × 104 to...

Labyrinthulomycete protists have gained significant attention in the recent past for their biotechnological importance. Yet, lipid profiles are poorly described because only a few large-scale isolation attempts been made so far. Here, we isolated more than 200 strains from mangrove habitats of China and characterized molecular phylogeny accumulation potential 71 strains. These were closest relatives six genera namely Aurantiochytrium, Botryochytrium, Parietichytrium, Schizochytrium,...

Abstract JAK/STAT pathway plays an important role in invertebrates during virus infection. However the microRNA (miRNA)-mediated regulation of is not intensively investigated. Viral miRNAs, encoded by genome, have emerged as regulators virus-host interactions. In this study, a WSSV (white spot syndrome virus)-encoded miRNA (WSSV-miR-22) was characterized shrimp The results showed that viral could promote infection targeting host STAT gene. When expression JAK or knocked down...

Autophagy is known to be involved in viral infection. However, the relationship between autophagy and infection has not been characterized. MicroRNAs (miRNAs), important regulators of gene transcription, play central roles many biologic processes, including It was therefore interest investigate miRNAs regulating host autophagy. In this study (white spot syndrome virus, WSSV) correlated positively with shrimp. Two (miR-71 -13b) had significant effects on MiR-71 regulated by targeting...

Nitrogen deprivation is known to improve lipid accumulation in microalgae and thraustochytrids. However, the patterns of fatty acid production molecular mechanisms underlying unsaturated saturated acids (SFAs) under nitrogen starvation remain largely unknown for In this study, batch culture experiments replete conditions were performed, changes transcriptome Aurantiochytrium sp. PKU#SW8 strain between these investigated. Our results showed improved yields total (TFAs), acids, SFAs...

Recently, there has been a burgeoning scholarly interest in elucidating the functional significance and regulatory mechanisms underlying involvement of small G proteins, such as Rab, antiviral immune response crustaceans. Rab is member protein family plays crucial role transport cell membranes within eukaryotic cells. It involved movement both on its surface, aiding entry effector proteins into specific membrane subregions. While previous research highlighted importance phagosome formation...

Microorganisms are essential for maintaining the ecological balance and supporting health of aquatic animals in aquaculture environments. This study utilized high-throughput sequencing technology to analyze diversity, composition, co-occurrence networks, assembly mechanisms, functional predictions bacterial communities seawater from both Mytilus coruscus areas (AA) non-aquaculture (NAA) across different seasons. The results indicated that number operational taxonomic units (OTUs) AA group...

Thraustochytrids are unicellular fungal-like marine protists with ubiquitous existence in environments. They well-known for their ability to produce high-valued omega-3 polyunsaturated fatty acids (ω-3-PUFAs) (e.g., docosahexaenoic acid (DHA)) and hydrolytic enzymes. Thraustochytrid biomass has been estimated surpass that of bacterioplankton both coastal oceanic waters indicating they have an important role microbial food-web. Nevertheless, the molecular pathway regulatory network PUFAs...