A5082562695- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Chemical Synthesis and Analysis
- Asymmetric Hydrogenation and Catalysis
- Biochemical and Molecular Research
- Biochemical and biochemical processes
- Innovative Microfluidic and Catalytic Techniques Innovation
- Patient Safety and Medication Errors
- Electrowetting and Microfluidic Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Cancer, Hypoxia, and Metabolism
- Cardiac, Anesthesia and Surgical Outcomes
- Viral Infectious Diseases and Gene Expression in Insects
- Pharmacogenetics and Drug Metabolism
- Click Chemistry and Applications
- CO2 Reduction Techniques and Catalysts
- GABA and Rice Research
- Phytochemicals and Antioxidant Activities
- Healthcare Technology and Patient Monitoring
- Syphilis Diagnosis and Treatment
- Venomous Animal Envenomation and Studies
- Phytochemistry and Biological Activities
- Carbon dioxide utilization in catalysis
- Biochemical and Structural Characterization
- Catalysis for Biomass Conversion
The King's College
2025
King's College London
2024
University of Cambridge
2021-2024
Child Trends
2022
University of Manchester
2014-2022
University of Lagos
2014
The imine reductase AoIRED from Amycolatopsis orientalis (Uniprot R4SNK4) catalyzes the NADPH-dependent reduction of a wide range prochiral imines and iminium ions, predominantly with (S)-selectivity ee's up to >99%. displays 100-fold greater catalytic efficiency for 2-methyl-1-pyrroline (2MPN) compared other IREDs, such as enzyme Streptomyces sp. GF3546, which also exhibits (S)-selectivity, thus, is an interesting candidate preparative synthesis. unusual properties, inversion...
Abstract The reductive aminase from Aspergillus oryzae ( Asp RedAm) was combined with a single alcohol dehydrogenase (either metagenomic ADH‐150, an ADH Sphingobium yanoikuyae (SyADH), or variant of the Thermoanaerobacter ethanolicus Te SADH W110A)) in redox‐neutral cascade for biocatalytic alkylation amines using primary and secondary alcohols. Aliphatic aromatic were obtained up to 99 % conversion, as well chiral directly racemic precursors >97 ee , releasing water only byproduct.
Reductive aminases (RedAms) catalyze the asymmetric reductive amination of ketones with primary amines to give secondary amine products. RedAms have great potential for synthesis bioactive chiral amines; however, insights into their mechanism are currently limited. Comparative studies on cyclohexanone allylamine in presence RedAms, imine reductases (IREDs), or NaBH3CN support distinctive activity catalyzing both formation and reduction reaction. Structures AtRedAm from Aspergillus terreus,...
Biocatalytic retrosynthetic analysis of dibenz[c,e]azepines has highlighted the use imine reductase (IRED) and ω-transaminase (ω-TA) biocatalysts to establish key stereocentres these molecules. Several enantiocomplementary IREDs were identified for synthesis (R)- (S)-5-methyl-6,7-dihydro-5H-dibenz[c,e]azepine with excellent enantioselectivity, by reduction parent imines. Crystallographic evidence suggests that may be able bind one conformer substrate such that, upon reduction, major product...
Abstract The NADP(H)‐dependent reductive aminase from Aspergillus oryzae ( Asp RedAm) was combined with an NADPH oxidase (NOX) to develop a redox system that recycles the co‐factor. RedAm‐NOX applied initially for kinetic resolution of variety racemic secondary and primary amines yield S ‐configured enantiomeric excess ee ) values up 99 %. addition ammonia borane this enabled efficient deracemization amines, including pharmaceutical drug rasagiline natural product salsolidine, conversions...
Enzymatic reductive amination catalysed by imine reductases (IREDs) and aminases (RedAms) provides green, stereoselective, direct access to 2° 3° chiral amines. Given existing interests in exploiting RedAms for commercial-scale synthesis of active pharmaceutical ingredients, developing efficient enantiodivergent RedAm systems would boost biocatalysis prospects early drug discovery. To develop adaptable systems, residues/motifs that can serve as handles stereocontrol this enzyme family need...
Asymmetric reductive amination catalysed by aminases (RedAms) provides a green and direct route to 2° 3° chiral amines. Identifying residues or motifs in these enzymes that facilitate stereocontrol...
Abstract Fungal ferulic acid decarboxylases (FDCs) belong to the UbiD‐family of enzymes and catalyse reversible (de)carboxylation cinnamic derivatives through use a prenylated flavin cofactor. The latter is synthesised by prenyltransferase UbiX. Herein, we demonstrate applicability FDC/UbiX expressing cells for both isolated enzyme whole‐cell biocatalysis. FDCs exhibit high activity with total turnover numbers (TTN) up 55000 frequency (TOF) 370 min −1 . Co‐solvent compatibility studies...
Abstract The reductive aminase from Aspergillus oryzae ( Asp RedAm) was combined with a single alcohol dehydrogenase (either metagenomic ADH‐150, an ADH Sphingobium yanoikuyae (SyADH), or variant of the Thermoanaerobacter ethanolicus Te SADH W110A)) in redox‐neutral cascade for biocatalytic alkylation amines using primary and secondary alcohols. Aliphatic aromatic were obtained up to 99 % conversion, as well chiral directly racemic precursors >97 ee , releasing water only byproduct.
Allylic amines are a versatile class of synthetic precursors many valuable nitrogen-containing organic compounds, including pharmaceuticals. Enzymatic allylic amination methods provide sustainable route to these compounds but often restricted primary amines. We report biocatalytic system for the reductive
Background: Natural products play a significant role in human therapy. They represent huge reservoir of bioactive chemical diversity and help understanding the cellular pathways that are essential component drug discovery process. Objective: This study was aimed at evaluating antimicrobial activity stigmasterol isolated from stem bark Neocarya macrophylla. Methods: Stigmasterol previously N. macrophylla subjected to screening against methicillin-resistant Staphylococcus aureus (MRSA),...
Robust<italic>in vitro</italic>and<italic>in vivo</italic>carboxylic acid reductase (CAR)-based biocatalytic systems have been developed that enable hydrogenation of α,β-unsaturated carboxylic acids to allylic alcohols and their saturated analogues.
Abstract Naturally occurring isoprenoid quinones mediate electron transfer in respiratory or photosynthetic chains of living organisms. Tremendous progress has been made the elucidation biosynthetic pathways prenylated and a number enzymes that catalyze specific transformation steps with remarkably high regio‐ stereoselectivity have characterized. Interestingly, some these possess broad substrate scope towards synthetic analogues, thereby enabling their application as tools. The availability...
Abstract Biocatalytic retrosynthetic analysis of dibenz[c,e]azepines has highlighted the use imine reductase (IRED) and ω‐transaminase (ω‐TA) biocatalysts to establish key stereocentres these molecules. Several enantiocomplementary IREDs were identified for synthesis ( R )‐ S )‐5‐methyl‐6,7‐dihydro‐5 H ‐dibenz[c,e]azepine with excellent enantioselectivity, by reduction parent imines. Crystallographic evidence suggests that may be able bind one conformer substrate such that, upon reduction,...