A5022821605- Reproductive Biology and Fertility
- Ovarian function and disorders
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Assisted Reproductive Technology and Twin Pregnancy
- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Prenatal Screening and Diagnostics
- Ectopic Pregnancy Diagnosis and Management
- Uterine Myomas and Treatments
- Gynecological conditions and treatments
- Renal and related cancers
- Birth, Development, and Health
- Coenzyme Q10 studies and effects
- Pregnancy and Medication Impact
- Pregnancy and preeclampsia studies
- Ovarian cancer diagnosis and treatment
- Mitochondrial Function and Pathology
- Microfluidic and Bio-sensing Technologies
- Immune Cell Function and Interaction
- Pluripotent Stem Cells Research
- Gestational Trophoblastic Disease Studies
- Genetics, Aging, and Longevity in Model Organisms
- Cellular Mechanics and Interactions
- Anesthesia and Neurotoxicity Research
University of Vermont
2020-2024
University of Vermont Medical Center
2018-2024
Dartmouth–Hitchcock Medical Center
2015-2023
Dartmouth College
2015-2020
University of Toronto
2006-2019
CReATe Fertility Centre
2019
McMaster University
2017-2018
Mount Sinai Hospital
2005-2015
Lunenfeld-Tanenbaum Research Institute
2005-2015
Centre for Social Innovation
2004-2013
This paper is the first report of robotic intracytoplasmic sperm injection (ICSI). ICSI a clinical procedure performed worldwide in fertility clinics, requiring pick-up single and insertion it into an oocyte (i.e., egg cell). Since its invention 20 years ago, has been conducted manually by handful highly skilled embryologists; however, success rates vary significantly among clinics due to poor reproducibility inconsistency across operators. We leverage our work cell realize aim ultimately,...
The age-related reduction in live-birth rate is attributed to a high of aneuploidy and follicle depletion. We showed an animal model that treatment with Coenzyme Q10 (CoQ10) markedly improved reproductive outcome. aim this study was compare the post-meiotic oocyte vitro fertilization (IVF) intra cytoplasmic sperm injection (ICSI) patients treated CoQ10 or placebo.We conducted double blind placebo controlled randomized trial included IVF-ICSI 35-43 years age. were either 600 mg equivalent...
ABSTRACT: The purpose of this study was to evaluate the ability spermatozoa and leukocytes in semen produce reactive oxygen species (ROS) by using nitroblue tetrazolium (NBT) staining examine association between NBT levels ROS as measured chemiluminescence. Twenty‐one infertility patients (leukocytospermia; n = 8; nonleukocytospermia, 13) 9 healthy donors were included. Standard analysis density gradient centrifugation performed test staining, ROS, total antioxidant capacity. A ROS‐total...
Manipulating single cells with a micropipette is the oldest, yet still widely used technique. This paper discusses aspiration of cell into and positioning accurately to target position inside micropipette. Due small volume (picoliter) nonlinear dynamics involved, these tasks have high skill requirements are labor intensive in manual operation that solely based on trial error has failure rates. We present automated techniques this for achieving via computer vision microscopy closed-loop...
ABSTRACT: The objective of this study was to determine the effects low‐level laser light exposure on motility spermatozoa and DNA damage. Thirty‐three semen samples were collected for routine analysis classified as normospermic, oligospermic, or asthenospermic. After performed, residual divided into treated control aliquots. Treated exposed a 30‐second infrared pulse 50 mW/cm 2 at 905 nm, wavelength thought increase light‐sensitive cytochrome c oxidase in mitochondrial electron transport...
Over the past four decades, due to cultural and social changes, women in developed world have significantly delayed childbirth. This trend is even worse for patients who attend infertility clinics. It well-known that live birth rates older than 35 are lower those younger, both naturally with assisted reproduction. Fertility decline is, part, an increase oocyte aneuploidy leads a reduced embryo quality, as well increased incidence of miscarriages defects. Here we show aging-associated...
Little is known about resident attitudes toward elective egg freezing (EF) or how educational exposure to EF affects residents' views and ability counsel patients. This study aimed evaluate US OB/GYN on EF, decisions regarding family planning, whether education these self-reported comfort discussing with A 32 question survey was emailed program directors at all residency programs for distribution residents. Chi-square tests were used the relationship between factors counselling Of those...
Sperm immobilization is a requisite step in intracytoplasmic sperm injection (ICSI). Conventionally, performed manually, which entails long training hours and stringent skills. Manual also has the limitation of low success rates poor reproducibility due to human fatigue skill variations across operators. This paper presents system for fully automated eliminate limitations manual operation. Integrating computer vision motion control algorithms, able visually track micropipette immobilize...
In Brief OBJECTIVE: To estimate the effect of protein supplementation culture media on reactive oxygen species production and incidence apoptosis in preimplantation mouse embryos. METHODS: A total 72 two-cell embryos were cultured human tubal fluid (HTF) alone (HTF-alone, control) 71 HTF with (10% serum substitute supplement; HTF-SSS) for hours. Total cell number per embryo was determined by staining Hoechst 33258. Allocation inner mass trophectoderm blastocysts confocal microscopy. Levels...