A5003044010- Enzyme Structure and Function
- Protein Structure and Dynamics
- Biosensors and Analytical Detection
- Analytical Chemistry and Sensors
- Liver Disease Diagnosis and Treatment
- Advanced Chemical Sensor Technologies
- Ultrasound in Clinical Applications
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Renal and Vascular Pathologies
- Amino Acid Enzymes and Metabolism
- Mass Spectrometry Techniques and Applications
- Pleural and Pulmonary Diseases
- Photonic and Optical Devices
- Olfactory and Sensory Function Studies
- Lung Cancer Diagnosis and Treatment
- Mycotoxins in Agriculture and Food
- Liver Disease and Transplantation
- Glycosylation and Glycoproteins Research
- Photoreceptor and optogenetics research
- Galectins and Cancer Biology
- Electrochemical sensors and biosensors
- Biochemical Analysis and Sensing Techniques
- Ultrasound and Hyperthermia Applications
- Indoor Air Quality and Microbial Exposure
Institute of Food Science
2015-2024
Institute of Atmospheric Sciences and Climate
2024
Federico II University Hospital
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2000-2023
Casa Sollievo della Sofferenza
2005-2023
University of Naples Federico II
1986-2023
Confederazione Nazionale dell'Artigianato e Della Piccola e Media Impresa
2023
National Research Council
2007-2020
Institute of Protein Biochemistry
2006-2019
Consorzio Roma Ricerche
2018
ER-TGN contact sites (ERTGoCS) have been visualized by electron microscopy, but their location in the crowded perinuclear area has hampered analysis via optical microscopy as well mechanistic study. To overcome these limits we developed a FRET-based approach and screened several candidates to search for molecular determinants of ERTGoCS. These included ER membrane proteins VAPA VAPB lipid transfer possessing dual (ER TGN) targeting motifs that hypothesized contribute maintenance ERTGoCS,...
Systemic sclerosis (SSc) patients in the early stages of pulmonary fibrosis (PF) often have few or no symptoms, normal to borderline function tests, and negative chest X-ray (CXR); high-resolution computed tomography (HRCT) is only reliable means detecting signs PF. However, thoracic ultrasound (TUS) enables detection pleural thickening, pleural/subpleural nodules, other subpleural lung abnormalities across 70% surface. We reassessed concordance between TUS HRCT findings SSc patients, see...
Abstract Damage-associated molecular patterns (DAMPs) are molecules that can be actively or passively released by injured tissues and activate the immune system. Here we show nicotinate phosphoribosyltransferase (NAPRT), detected antibody-mediated assays mass spectrometry, is an extracellular ligand for Toll-like receptor 4 (TLR4) a critical mediator of inflammation, acting as DAMP. Exposure human mouse macrophages to NAPRT activates inflammasome NF-κB secretion inflammatory cytokines....
The demand for a wide choice of food that is safe and palatable increases every day. Consumers do not accept off-flavors have atypical odors resulting from internal deterioration or contamination by substances alien to the food. Odor response depends on volatile organic compounds (VOCs), their detection can provide information about quality. Gas chromatography/mass spectrometry most powerful method available VOC. However, it laborious, costly, requires presence trained operator. To develop...
The aim of this study was to investigate the clinical applicability transthoracic ultrasound (TUS) in diagnosis and follow-up community acquired pneumonia (CAP).We designed a pilot 15 patients subsequently investigated 342 (206 men 136 women) consecutively admitted our Department from September 2005 November 2009 because radiographically diagnosed CAP. All underwent standard chest radiography, consequently TUS. Follow-up TUS were performed at 4th 8-10th day, most patients.Concerning...
BACKGROUND Serum α-L-fucosidase activity is considered a marker of hepatocellular carcinoma. To the authors' knowledge, its clinical usefulness in early detection carcinoma follow-up cirrhotic patients has not been reported previously. METHODS The authors prospectively studied serum activity, addition to α-fetoprotein and ultrasonography, regular screening 132 during an 8-year follow-up. RESULTS At enrollment, 120 had low (below cutoff value) 12 high activity. All levels below value. During...
Background Recent reports indicate that numerical assessment of B-lines during transthoracic ultrasound may aid the differential diagnosis acute diffuse pleuropulmonary disorders. Purpose To determine whether are different in normal and diseased lungs they can be used to discriminate between types pulmonary disorders acutely ill patients. Material Methods In this multicenter study, ultrasonography was performed on 193 patients with dyspnea, healthy non-smokers, 58 who had undergone...
The value of serum alpha-L-fucosidase activity in the diagnosis hepatocellular carcinoma (HCC) was investigated by determining enzyme levels 21 patients with HCC, 76 cirrhosis, 22 other malignant neoplasms, and 23 healthy subjects.The level HCC (575.76 +/- 212.86 nmol/ml/h) significantly higher than that found cirrhosis (274.55 138.97 nmol/ml/h; P less 0.001) or neoplasms (257.91 128.12 controls (221.23 114.45 0.001). No significant differences were between neoplasms. When 443 nmol/ml/h is...
Abstract Detection of ciprofloxacin residues in milk by sensitive and rapid methods is great interest due to its use the treatment dairy livestock health. Current analytical approaches antibiotics detection, are laboratory-based they time-consuming require trained personnel. To cope this problem, we propose an assay, based on fluorescence polarization principle, able detect presence diluted sample without any pre-treatment. The proposed method ciprofloxacin-protein conjugate labeled with...
In this paper, we report, for the first time to our knowledge, a new versatile and future proof diagnostic platform diverse applications heterogeneous biological targets. This relies on plasmonic-augmented silicon photonic biochip detect bacteria protein biomarkers within minutes. To demonstrate potential of in applications, detection two targets, bacterium Escherichia coli (E. coli) molecule C-reactive (CRP) using universal method. E. is one most commonly encountered bacterial pathogens...
Abstract Despite the fact that porcine odorant‐binding protein (pOBP) possesses a single tryptophan residue (Trp 16) is characterized by high density microenvironment (80 atoms in sphere with radius 7 Å) only one polar group (Lys 120) and three bound water molecules, pOBP displayed red shifted fluorescence emission spectrum (λ max = 340 nm). The unfolding 5 M GdnHCl was accompanied shift of 353 nm), increase quantum yield, decrease lifetime excited state (from 4.25 ns native to 3.15 presence...
Steroids are a class of hormones improperly used in livestock as growth-promoting agents. Due to their high risk for human health, the European Union (EU) has strictly forbidden administration all natural and synthetic steroid food-producing animals, development new rapid detection methods greatly encouraged. This work reports novel fluorescence polarization assay, ready use, capable detecting 17β-estradiol directly milk samples with low limit <10 pmol. It is based on coupling monospecific...