A5015218473- Enzyme Structure and Function
- Protein Structure and Dynamics
- Amino Acid Enzymes and Metabolism
- Advanced biosensing and bioanalysis techniques
- Advanced Chemical Sensor Technologies
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Olfactory and Sensory Function Studies
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Biochemical effects in animals
- Photoreceptor and optogenetics research
- Noise Effects and Management
- Biotin and Related Studies
- Celiac Disease Research and Management
- Advanced Fluorescence Microscopy Techniques
- Mass Spectrometry Techniques and Applications
- Diet, Metabolism, and Disease
- Bacterial Genetics and Biotechnology
- Protein Interaction Studies and Fluorescence Analysis
- Glycosylation and Glycoproteins Research
- Biochemical Analysis and Sensing Techniques
- bioluminescence and chemiluminescence research
- Smart Materials for Construction
- Biochemical and Molecular Research
Institute of Food Science
2009-2024
Institute of Atmospheric Sciences and Climate
2024
Confederazione Nazionale dell'Artigianato e Della Piccola e Media Impresa
2023
National Research Council
2004-2020
Shaanxi Normal University
2019
Cambridge University Press
2019
Universidad Nacional de La Plata
2019
Institute of Protein Biochemistry
2005-2015
National Academies of Sciences, Engineering, and Medicine
2006-2015
University of Siena
2010-2012
The demand for a wide choice of food that is safe and palatable increases every day. Consumers do not accept off-flavors have atypical odors resulting from internal deterioration or contamination by substances alien to the food. Odor response depends on volatile organic compounds (VOCs), their detection can provide information about quality. Gas chromatography/mass spectrometry most powerful method available VOC. However, it laborious, costly, requires presence trained operator. To develop...
The arginine binding protein from Thermatoga maritima (TmArgBP), a substrate (SBP) involved in the ABC system of solute transport, presents number remarkable properties. These include an extraordinary stability to temperature and chemical denaturants tendency form multimeric structures, uncommon feature among SBPs transport. Here we report biophysical structural characterization TmArgBP dimer. Our data indicate that dimer is endowed with since its full dissociation requires high as well SDS...
Abstract Detection of ciprofloxacin residues in milk by sensitive and rapid methods is great interest due to its use the treatment dairy livestock health. Current analytical approaches antibiotics detection, are laboratory-based they time-consuming require trained personnel. To cope this problem, we propose an assay, based on fluorescence polarization principle, able detect presence diluted sample without any pre-treatment. The proposed method ciprofloxacin-protein conjugate labeled with...
Abstract Despite the fact that porcine odorant‐binding protein (pOBP) possesses a single tryptophan residue (Trp 16) is characterized by high density microenvironment (80 atoms in sphere with radius 7 Å) only one polar group (Lys 120) and three bound water molecules, pOBP displayed red shifted fluorescence emission spectrum (λ max = 340 nm). The unfolding 5 M GdnHCl was accompanied shift of 353 nm), increase quantum yield, decrease lifetime excited state (from 4.25 ns native to 3.15 presence...
Steroids are a class of hormones improperly used in livestock as growth-promoting agents. Due to their high risk for human health, the European Union (EU) has strictly forbidden administration all natural and synthetic steroid food-producing animals, development new rapid detection methods greatly encouraged. This work reports novel fluorescence polarization assay, ready use, capable detecting 17β-estradiol directly milk samples with low limit <10 pmol. It is based on coupling monospecific...
In this work, the binding of recombinant glutamine-binding protein (GlnBP) from Escherichia coli to gliadin peptides, toxic for celiac patients, was investigated by mass spectrometry experiments and optical techniques. Mass demonstrated that GlnBP binds following amino acid sequence: XXQPQPQQQQQQQQQQQQL, present only into prolamines. The suggested us design a new biosensor based on nanostructured porous silicon (PSi) detection trace amounts in food. GlnBP, which acts as molecular probe...
The detection of hazard exposure is a current priority, including the traces explosive molecules in different environments like luggage storage rooms and public places, becoming major requirement for homeland security. In present study we carried out preliminary investigation on binding capacities four forms lipocalin odorant-binding protein (OBP) components such as diphenylamine, dimethyl-phthalate, resorcinol dinitrotoluene. experimental results, showing that OBP binds these compounds with...
The denaturation process of porcine odorant-binding protein (pOBP) was studied by intrinsic fluorescence analysis and far- near-UV circular dichroism measurements. Our results showed that a reversible one-step described the GdnHCl. midpoint transition, is, point where free energies in native unfolded states are equal, corresponds to 2.3 M difference energy between pOBP is −5.95 kcal/mol absence GdnHCl, indicating molecule very stable denaturing action A 15% increase intensity accompanied 25%...
Reported in this study are the experimental design and results of a protein-based biosensor for detection cadmium water using reversed-displacement format. This methodology has successfully measured by direct injection, eliminating need preconcentration or pretreatment samples. A column containing Chelex resin saturated with Zn2+ rodhamine-labeled metallothionein (MT) comprised assay reactive chamber. In fact, MT small cysteine-rich proteins that bind heavy metals such as zinc, cadmium,...
In this work, we describe how to realize a new sensing platform for an easy and fast detection of analytes. particular, utilized enhanced fluorescence emission on silver island films (SIFs) coupled the total internal reflection mode (TIRF) develop assay format target Here, as example, report toxic peptides present in gliadin (Gli). Our was performed follows: (1) first captured surfaces coated with anti-Gli antibodies; (2) were then incubated fluorophore-labeled (3) signal from antibody bound...
The final goal of this work is to achieve a selective detection butanal by the realization simple, small-size and low cost experimental approach. To end, porcine odorant-binding protein was used in connection with surface plasmon resonance transduction plastic optical fiber tool for competitive assay. This allows reduce size sensing device it offers possibility design "Lab-on-a-chip" platform. obtained results showed that system approach able selectively detect presence concentration range...
In this paper, we report, for the first time to our knowledge, a new versatile and future proof diagnostic platform diverse applications heterogeneous biological targets. This relies on plasmonic-augmented silicon photonic biochip detect bacteria protein biomarkers within minutes. To demonstrate potential of in applications, detection two targets, bacterium Escherichia coli (E. coli) molecule C-reactive (CRP) using universal method. E. is one most commonly encountered bacterial pathogens...