The human host cell factor (HCF) is expressed in a variety of adult and fetal tissues, its gene conserved animals as diverse mammals insects. However, only known function to stabilize the herpes simplex virus virion transactivator VP16 complex with cellular POU domain protein Oct-1 cis-acting regulatory elements promoters immediate-early viral genes. To identify for HCF, we used yeast two-hybrid system ligand HCF. This protein, Luman, appears be cyclic AMP response element (CRE)-binding...

Luman/CREB3 (also called LZIP) is an endoplasmic reticulum (ER) membrane-bound transcription factor which believed to undergo regulated intramembrane proteolysis in response cellular cues. We previously found that Luman activates from the unfolded protein element. Here we report identification of Herp, a gene involved ER stress-associated degradation (ERAD), as direct target Luman. was transcriptionally induced and proteolytically activated by stress inducer thaspsigargin. Overexpression...

Background and objective Most previous studies adopted single traditional time series models to predict incidences of malaria. A model cannot effectively capture all the properties data structure. However, a stacking architecture can solve this problem by combining distinct algorithms models. This study compares performance deep learning in malaria case prediction explores application value methods field infectious disease prediction. Methods The ARIMA, STL+ARIMA, BP-ANN LSTM network were...

Background Osteoarthritis (OA) is a common aging-related degenerative joint disease with chronic inflammation as its possible pathogenesis. Oroxin B (OB), flavonoid isolated from traditional Chinese herbal medicine, possesses anti-inflammation properties which may be involved in regulating the pathogenesis of OA, but mechanism has not been elucidated. Our study was first to explore potential chondroprotective effect and elucidate underlying OB OA. Methods In vitro , primary mice chondrocytes...

Ferroptosis, as an iron-dependent form of necrotic cell death, has been reported to affect activities nucleus pulposus cells (NPCs). However, its role in the pathogenesis intervertebral disc degeneration (IDD) is largely unknown. Notably, our bioinformatics analysis predicted that circ_0072464 was downregulated IDD mice. Therefore, this study aimed at clarifying mechanisms extracellular vesicle- (EV-) encapsulated derived from bone marrow mesenchymal stem (BMSCs) NPC ferroptosis IDD. EVs...

The incidence of intervertebral disc degeneration (IVDD) is a common degenerative disease with inflammation, decreased autophagy, and progression fibrosis as its possible pathogenesis. Physalin A (PA) widely studied anti-inflammatory drug. However, therapeutic effects on IVDD remain unexplored. Therefore, we aimed to explore the potential PA in progression. In vivo, investigated bioactivity using puncture-induced rat model. signals height changes were detected X-ray, micro-CT, MRI,...

Osteoarthritis (OA) has become the most common degenerative disease in world, which brings a serious economic burden to society and country. Although epidemiological studies have shown that occurrence of osteoarthritis is associated with obesity, sex, trauma, biomolecular mechanisms for development progression remain ambiguous. Several drawn connection between SPP1 osteoarthritis. was first found be highly expressed osteoarthritic cartilage, later more also subchondral bone synovial OA...

Osteoarthritis (OA) is a trauma-/age-related degenerative disease characterized by chronic inflammation as one of its pathogenic mechanisms. Mulberroside A (MA), natural bioactive withanolide, demonstrates anti-inflammatory properties in various diseases; however, little known about the effect MA on OA. We aim to examine role OA and identify potential mechanisms through which it protects articular cartilage. In vitro, improved inflammatory response, anabolism, catabolism IL-1β-induced...

Calpain-mediated proteolysis is one of the major regulatory factors for activity ATP-binding cassette transporter (ABC) A1. Helical apolipoproteins protect ABCA1 against this degradation and increase generation HDL. We investigated mechanism reaction focusing on roles endocytotic internalization ABCA1.Surface was labeled with biotin traced its degradation. in cell surface internalized within 10 minutes regardless presence apoA-I. intracellularly degraded protected only when exposed to...

Luman/CREB3 (also called LZIP) is an endoplasmic reticulum (ER)-bound cellular transcription factor. It has been implicated in the mammalian unfolded protein response (UPR), as well herpes simplex virus reactivation from latency sensory neurons. Here, we report identification of a novel Luman recruitment factor (LRF). Like Luman, LRF UPR-responsive basic-region leucine zipper that prone to proteasomal degradation. Being highly unstable protein, interacts with through region and promotes was...

Background: Since chronic hyperglycemia-related damage to small blood vessels results in complications, patients with longer durations of type 2 diabetes mellitus (T2DM) are more likely develop microvascular such as retinopathy, neuropathy and nephropathy, which very harmful the health humans. Therefore, this study aimed assess prevalence diabetes-related complications explore their risk factors T2DM Tianjin, China. Methods: This observational, cross-sectional was conducted at 8 hospitals...

Aims Osteoarthritis (OA) is a prevalent joint disorder with inflammatory response and cartilage deterioration as its main features. Dihydrocaffeic acid (DHCA), bioactive component extracted from natural plant ( gynura bicolor ), has demonstrated anti-inflammatory properties in various diseases. We aimed to explore the chondroprotective effect of DHCA on OA potential mechanism. Methods In vitro, interleukin-1 beta (IL-1β) was used establish mice chondrocytes. Cell counting kit-8 evaluated...

ABSTRACT The cascade of herpes simplex virus (HSV) gene expression that results in viral replication begins with the activation immediate-early (IE) genes by virion-associated protein VP16. VP16 on its own is inefficient at associating complexes formed IE promoters and depends upon cellular factor HCF for activity. In this respect mimics host basic leucine zipper (bZIP) Luman, which also requires activating transcription. Our objective to explore interactions between Luman determine if they...

ABSTRACT In human cells infected with herpes simplex virus (HSV), viral gene expression is initiated by the virion protein VP16. VP16 does not bind DNA directly but forms a multiprotein complex on immediate-early promoters two cellular proteins: POU domain Oct-1 and host cell factor (HCF; also called C1, VCAF, CFF). Despite its apparent role in stabilizing VP16-induced transcription complex, natural biological of HCF unclear. Only recently has been implicated control cycle. To determine...

Accumulating evidence indicates that intervertebral disc degeneration (IDD) is associated with diabetes mellitus (DM), while the underlying mechanisms still remain elusive. Herein, current study sought to explore potential molecular mechanism of IDD in diabetic rats based on transcriptome sequencing data.Streptozotocin (STZ)-induced type 1 (T1DM) were used obtain nucleus pulposus tissues for sequencing. Next, differentially expressed genes (DEGs) data and GSE34000 microarray dataset obtained...

TANK-binding kinase 1 (TBK1) is pivotal in autoimmune and inflammatory diseases, yet its role osteoarthritis (OA) remains elusive. This study sought to elucidate the effect of TBK1 inhibitor BX795 on OA delineate underlying mechanism by which it mitigates OA.

Expression of ABCA1 is regulated by transcription the gene and calpain-mediated proteolytic degradation, inhibition degradation results in increased HDL biogenesis vitro. We examined whether this approach could be a potential antiatherogenic treatment. Although probucol inhibits both activity ABCA1, its oxidized products, spiroquinone diphenoquinone, reduce without inhibiting or altering gene. Accordingly, compounds enhanced apolipoprotein A-I/ABCA1-dependent generation vitro, hepatic plasma...

Interleukin-1β (IL-1β)-induced inflammatory response is associated with osteoarthritis (OA) and its development. Histone deacetylase (HDAC) may be involved in regulating this pathogenesis, but the mechanism has yet to elucidated. The aim of present study was investigate underlying regulation IL‑1β‑stimulated catabolic degradation cartilage by HDAC. An vitro model OA generated using rat articular chondrocytes (rACs) treated IL‑1β. role HDAC IL‑1β‑induced gene expression investigated...

Aims Pellino1 (Peli1) has been reported to regulate various inflammatory diseases. This study aims explore the role of Peli1 in occurrence and development osteoarthritis (OA), so as find new targets for treatment OA. Methods After inhibiting expression chondrocytes with small interfering RNA (siRNA), interleukin (IL)-1β was used simulate inflammation, OA-related indicators such synthesis, decomposition, apoptosis were detected. Toll-like receptor (TLR) nuclear factor-kappa B (NF-κB)...

<title>Abstract</title> <bold>Objective</bold> Immune checkpoint inhibitors (ICIs) have come into widespread use in oncology recent years and are associated with rare cardiotoxicity, including potentially fatal myocarditis. To date, no model of the occurrence major adverse cardiovascular events (MACE) ICI‐related myocarditis has been constructed. In this paper, construet a machine-learning-based through diagnosis treatment data patients ICI-related to predict MACE patients....