Background Mercury-polluted environments are often contaminated with other heavy metals. Therefore, bacteria resistance to several metals may be useful for bioremediation. Cupriavidus metallidurans CH34 is a model metal-resistant bacterium, but possesses low mercury compounds. Methodology/Principal Findings To improve inorganic and organic of strain CH34, the IncP-1β plasmid pTP6 that provides novel merB, merG genes additional mer was introduced into bacterium by biparental mating. The...

The acidophilic proteobacterium Acidithiobacillus ferrooxidans is involved in the industrial biorecovery of copper. It found acidic environments biofilms and important biogeochemical cycling metals nutrients. Its genome contains a cluster four genes, glyQ, glysS, gph, act, that are predicted to encode alpha beta subunits glycine tRNA synthetase, phosphatase, an acyltransferase, respectively (GenBank accession no. DQ149607). cloned expressed Escherichia coli, produces acyl homoserine lactones...

Abstract The thermal decomposition of normal alkanes and linear poly(α‐olefins) was found to occur predominately by random scissions, followed volatilization into an open system. To show these processes the fragment distribution from high molecular weight polyethylene (fragments C 55 ), n ‐alkanes, polypropylene, polyisobutylene 60 ) are reported discussed in depth. Based on results, a mechanism scissions is postulated for ‐alkanes as well polypropylene. Polystyrene shown not follow same...

Nitrogen cycle microorganisms are essential in agricultural soils and may be affected by mercury pollution. The aims of this study to evaluate the bioremediation mercury-polluted soil using Cupriavidus metallidurans MSR33 a rotary drum bioreactor (RDB) characterize effects pollution on nitrogen microorganisms. An was contaminated with (II) (20–30 ppm) subjected strain custom-made RDB. were studied qPCR. Bioremediation RDB removed 82% mercury. cell concentrations, thioglycolate,...

Abstract Polypropylene mit bekannter Taktizität wurden durch Pyrolyse angeschlossener Hydrierung der Fragmente und temperaturprogrammierter Gaschromatographie untersucht. Ziel war eine Erweiterung bei Polyäthylen gewonnenen Kenntnisse über den Abbau erhöhten Temperaturen sowie die Bestimmung konfigurativer Sequenzverteilungen. Die Fragmentverteilung läßt sich — wie von aus Überlagerung einer statistischen nichtstatistischen Spaltung interpretieren. Aus dem Verlauf im Bereich langer...

In order to characterize the metabolites produced in vivo by biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase, first two enzymes of (polychloro)biphenyl catabolic pathway encoded bph locus Pseudomonas sp. LB400, recombinant E. coli strains expressing respective genes were constructed. Biphenyl-2,3-dioxygenase attack on 2,2′- or 2,4′-dichlorobiphenyl was shown give rise virtually quantitative ortho-dechlorination these congeners hydroxylation at chlorinated carbon 2 its...

Ga-doped Nd-Fe-B sintered magnets show an improved wetting behavior of the liquid phase during sintering and formation additional Ga-containing intergranular phases. Nb forms precipitates within hard magnetic grains enhances coercivity. In as-sintered magnets, a new Nd-rich region which contains Nd/sub 3/Ga 5/Ga/sub 3/ is found. This formed from cooling. addition, delta -phase occurs in annealed magnets. found hexagonal NbFeB boride small Nb-containing phase. There no detected phases.< <ETX...

Abstract A system with flash pyrolysis and gas chromatography (PGC) which includes in‐line hydrogenation packed columns is described. Under optimal conditions of temperature programming flow rate, a high degree resolution was obtained over broad range fragment lengths. The has been applied to the analysis side groups long branches in polyethylene. It found that, for low concentration branches, yield distribution isoalkanes very sensitive type polymerization used form great number...

Abstract Der Abbau von linearem Polyäthylen (PE) vom Ziegler‐Typ und n ‐Alkanen wurde im Hinblick auf Spaltungsmechanismen Vinylpolymeren ihre Anwendung zur Ermittlung Sequenzlängenverteilungen theoretisch untersucht. Die Pyrolyse erfolgte bei erhöhter Temperatur innerhalb weniger Sekunden war gekoppelt mit der Hydrierung Fragmente linear temperaturprogrammierter Gaschromatographie. Durch Verbesserungen Technik konnte die Fragmentverteilung bis C 30 FID (Flammenionisationsdetektor)...

Bioremediation of sites polluted with petroleum hydrocarbons and heavy metals is a major challenge.The aim this study was the isolation characterization hydrocarbon-degrading metal-tolerant bacteria.Sixteen hydrocarbonoclastic bacteria were isolated by enrichment from crude oil-contaminated soil at Aconcagua river mouth, Central Chile.Most strains cocci-shaped exhibited circular cream-colored colonies smooth texture.Isolates resistant to bacitracin penicillin, two isolates motile.Isolates...

Tomato crops can be affected by several infectious diseases produced bacteria, fungi, and oomycetes. Four phytopathogens are of special concern because the major economic losses they generate worldwide in tomato production; Clavibacter michiganensis subsp. Pseudomonas syringae pv. tomato, causative agents behind two highly destructive diseases, bacterial canker speck, respectively; fungus Fusarium oxysporum f. sp. lycopersici that causes Wilt, which strongly affects crops; finally,...

The genus Clavibacter has been associated largely with plant diseases. aims of this study were to characterize the genomes and virulence factors Chilean C. michiganensis subsp. strains VL527, MSF322 OP3, define their phylogenomic positions within species, michiganensis. VL527 possess 3,396,632 3,399,199 bp, respectively, a pCM2-like plasmid in strain pCM1- plasmids MSF322. OP3 genome is composed chromosome three (including plasmids) 3,466,104 bp. Genomic analyses confirmed phylogenetic...

Aconcagua River basin (Central Chile) harbors diverse economic activities such as agriculture, mining and a crude oil refinery. The aim of this study was to assess environmental drivers microbial communities in estuarine soils, which may be influenced by anthropogenic taking place upstream natural processes tides flood runoffs. Physicochemical parameters were measured floodplain soils along the estuary. Bacteria, Actinobacteria, Alphaproteobacteria, Betaproteobacteria, Pseudomonas, Bacillus...

To evaluate the in vitro and vivo antifungal capability of diverse compost teas endemic Chilean flora inoculated with a consortium fungal strains Trichoderma spp. (biocontrol agent) against three important phytopathogens: Botrytis cinerea, Fusarium oxysporum, andLasiodiplodia theobromae.Compost were obtained from Chile (Azara celastrina, Citronella mucronate, Cryptocarya alba, Peumus boldus, Quillaja saponaria). Eleven isolated, antagonism tests performed to develop consortiums biocontrol...

In this study, spontaneous synthesis of a gold (Au) colloid using cells Cupriavidus metallidurans CH34 is reported, and compared with results obtained the model bacterium Escherichia coli MG1655. To investigate mechanism, bacterial biomass secretomes from both strains were incubated Au(III) ions. Only capable producing extracellular dispersions Au nanoparticles (NPs). Transmission electron microscopy images showed that AuNPs morphology was dominated by triangular decahedral nanostructures....

Mercury is a toxic element that harms organisms and disturbs biogeochemical cycles. bioremediation based on the reduction of Hg (II) to (0) by mercury-resistant bacteria. Cupriavidus metallidurans MSR33 possesses broad-spectrum mercury resistance. This study aims establish effects growth, oxygen uptake, removal parameters C. in aqueous solution during aerobic anaerobic bioremediation. A new culture medium (GBC) was designed. The (20 ppm) growth parameters, were evaluated GBC bioreactor (3 L)...

Abstract A series of methyl methacrylate (MMA)–methacrylic acid (MAA) copolymers containing up to 50% methacrylic and the respective homopolymers were reproducibly pyrolyzed at 900°C fragments identified by gas chromatography (GC) or GC–mass spectroscopy. It was shown that PMMA MMA portions blocky random yielded 99% MMA, while a large portion (50–60%) MAA broke down give wide variety hydrocarbons via decarboxylation and/or anhydride formation. Both unsaturated aliphatic aromatic...

Bacterial canker caused by Clavibacter michiganensis subsp. (Cmm) is one of the most important bacterial diseases tomato. In order to understand emergence and dissemination this pathogen in Chile, Chilean Cmm strains isolated from different sites central Chile were characterized compared a worldwide collection strains. Multilocus sequence analysis typing ( MLSA ‐ MLST ) based on five housekeeping genes, i.e. atpD , dnaK gyrB ppk recA multilocus variable number tandem repeats VNTR MLVA eight...