A5027325862- Adipose Tissue and Metabolism
- Cardiovascular and Diving-Related Complications
- Marine animal studies overview
- High Altitude and Hypoxia
- Physiological and biochemical adaptations
- Nitric Oxide and Endothelin Effects
- Ocean Acidification Effects and Responses
- Redox biology and oxidative stress
- Mitochondrial Function and Pathology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Muscle Physiology and Disorders
- Neuroscience of respiration and sleep
- Fatty Acid Research and Health
- Cancer, Hypoxia, and Metabolism
- Effects and risks of endocrine disrupting chemicals
- Aquaculture disease management and microbiota
- Eicosanoids and Hypertension Pharmacology
- Heme Oxygenase-1 and Carbon Monoxide
- Ferroptosis and cancer prognosis
- Mercury impact and mitigation studies
- Spaceflight effects on biology
- Pluripotent Stem Cells Research
- Environmental Toxicology and Ecotoxicology
- RNA modifications and cancer
- Computational Physics and Python Applications
University of California, Berkeley
2017-2024
Berkeley College
2020-2023
Integra (United States)
2018-2022
Acupuncture & Integrative Medicine College
2022
University of California System
2022
University of California, Merced
2009-2021
University of Pennsylvania
2013-2019
California University of Pennsylvania
2014-2017
Philadelphia University
2016
Institute for Environmental Management
2016
Peroxiredoxin 6 (Prdx6) repairs peroxidized membranes by reducing oxidized phospholipids, and replacing sn-2 fatty acyl groups through hydrolysis/reacylation its phospholipase A2 (aiPLA2) lysophosphatidylcholine acyltransferase activities. Prdx6 is highly expressed in the lung, intact lungs cells null for or with single-point mutations that inactivate either Prdx6-peroxidase aiPLA2 activity alone exhibit decreased viability, increased lipid peroxidation, incomplete repair when exposed to...
Peroxiredoxin 6 (Prdx6) is essential for activation of NADPH oxidase type 2 (NOX2) in pulmonary microvascular endothelial cells (PMVECs), alveolar macrophages (AMs), and polymorphonuclear leukocytes. Angiotensin II phorbol ester increased superoxide/H2O2 generation PMVECs, AMs, isolated lungs from wild-type (WT) mice, but had much less effect on or Prdx6-null Prdx6-D140A-knock-in mice that lack the phospholipase A2 activity (PLA2) Prdx6; addition either lysophosphatidylcholine (LPC)...
Elephant seals are naturally adapted to survive up three months of absolute food and water deprivation (fasting). Prolonged in terrestrial mammals increases reactive oxygen species (ROS) production, oxidative damage inflammation that can be induced by an increase the renin-angiotensin system (RAS). To test hypothesis prolonged fasting elephant is not associated with increased stress or inflammation, blood samples muscle biopsies were collected from early (2-3 weeks post-weaning) late (7-8...
Northern elephant seals experience prolonged periods of absolute food and water deprivation (fasting) while breeding, molting or weaning. The postweaning fast in is characterized by increases the renin-angiotensin system, expression oxidant-producing protein Nox4, NADPH oxidase activity; however, these are not correlated with increased oxidative damage inflammation. Glutathione (GSH) a potent reductant cofactor for glutathione peroxidases (GPx), glutathione-S transferases (GST) 1-cys...
Activation of angiotensin receptor type 1 (AT1) contributes to NADPH oxidase (Nox)-derived oxidative stress during metabolic syndrome. However, the specific role AT1 in modulating redox signaling, mitochondrial function, and heart remains more elusive. To test hypothesis that activation increases while impairing signaling function diet-induced insulin resistance obese animals, Otsuka Long Evans Tokushima Fatty (OLETF) rats (n = 8/group) were treated with blocker (ARB) olmesartan for 6 wk....
The trade-off between current reproductive effort and survival is a key concept of life history theory. A variety studies support the existence this but underlying physiological mechanisms are not well-understood. Oxidative stress has been proposed as potential mechanism observed inverse relationship investment lifespan. Prolonged fasting associated with oxidative including increases in production reactive oxygen species, damage inflammation.Northern elephant seals (NES) undergo prolonged...
Peroxiredoxin 6 (Prdx6) is a multifunctional enzyme that serves important antioxidant roles by scavenging hydroperoxides and reducing peroxidized cell membranes. Prdx6 also plays key role in signaling activating the NADPH oxidase, type 2 (Nox2) through its acidic Ca 2+ -independent phospholipase A2 (aiPLA2) activity. Nox2 generation of O ·− , addition to signaling, can contribute oxidative stress inflammation such as during sepsis-induced acute lung injury (ALI). To evaluate possible...
Abstract Background Elephant seals exhibit extreme hypoxemic tolerance derived from repetitive hypoxia/reoxygenation episodes they experience during diving bouts. Real-time assessment of the molecular changes underlying protection against hypoxic injury in remains restricted by their at-sea inaccessibility. Hence, we developed a proliferative arterial endothelial cell culture model elephant and used RNA-seq, functional assays, confocal microscopy to assess response prolonged hypoxia. Results...
Postprandial cellular glucose uptake is dependent on an insulin-signaling cascade in muscle and adipose tissue, resulting the translocation of insulin-dependent transporter 4 (Glut4) into plasma membrane. Additionally, extended food deprivation characterized by suppressed insulin signaling decreased Glut4 expression. Northern elephant seals are adapted to prolonged fasts high levels glucose. To address hypothesis that fasting-induced decrease associated with reduced fasted seals, we compared...
Extended breath-hold (apnea) bouts are routine during diving and sleeping in seals. These apneas result oxygen store depletion blood flow redistribution towards obligatory oxygen-dependent tissues, exposing seals to critical levels of ischemia hypoxemia. The subsequent reperfusion/reoxygenation has the potential increase oxidant production thus oxidative stress. contributions extended apnea stress adapted mammals not well defined. To address hypothesis that is associated with increased...
The northern elephant seal pup (Mirounga angustirostris) undergoes a 2-3 month post-weaning fast, during which it depends primarily on the oxidation of fatty acids to meet its energetic demands. concentration non-esterified (NEFAs) increases and is associated with development insulin resistance in late-fasted pups. Furthermore, plasma NEFA concentrations respond differentially an intravenous glucose tolerance test (ivGTT) depending fasting duration, suggesting that effects lipid metabolism...
Maturation in hooded seals is characterized by the rapid development of their physiological diving capacity and accompanied increases oxidant production but not oxidative damage. To test hypothesis that antioxidant system develops as they transition from a terrestrial to an aquatic environment, we obtained complete cDNA sequence encodes NF-E2-related factor 2 (Nrf2), central regulator response, compared Nrf2 mRNA protein expression levels muscle samples neonate, weaned pups adult seals,...
Prolonged food deprivation increases lipid oxidation and utilization, which may contribute to the onset of insulin resistance associated with fasting. Because promotes preservation glucose fat, it has been suggested be an adaptive response deprivation. However, fasting mammals exhibit hypoinsulinemia, suggesting that resistance-like conditions they experience actually result from reduced pancreatic sensitivity glucose/capacity secrete insulin. To determine whether results in or dysfunction,...
Lungs stored ahead of transplant surgery experience ischemia. Pulmonary ischemia differs from in the systemic organs that stop blood flow lung leads to loss shear alone because parenchyma does not rely on for its cellular oxygen requirements. Our earlier studies ischemia-induced mechanosignaling cascade showed pulmonary endothelium responds by production reactive species (ROS). We hypothesized ROS produced this way led induction proinflammatory mediators. In study, we used lungs or cells...