A5022763953- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Asymmetric Hydrogenation and Catalysis
- Microbial Natural Products and Biosynthesis
- Chemical Synthesis and Analysis
- Phytochemicals and Antioxidant Activities
- Plant biochemistry and biosynthesis
- Food Quality and Safety Studies
- Plant Gene Expression Analysis
- Polysaccharides and Plant Cell Walls
- Fermentation and Sensory Analysis
- Microbial Metabolism and Applications
- Advanced Cellulose Research Studies
- Polysaccharides Composition and Applications
- Nanomaterials for catalytic reactions
- Biochemical and biochemical processes
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Microbial Metabolites in Food Biotechnology
- Cancer Research and Treatments
- Ginkgo biloba and Cashew Applications
- Microbial Inactivation Methods
- Genomics, phytochemicals, and oxidative stress
- Tea Polyphenols and Effects
Jiangnan University
2019-2025
State Key Laboratory of Food Science and Technology
2023-2024
University of Maryland, Baltimore County
2019-2021
Nanjing Tech University
2013-2015
Violacein is a naturally occurring anticancer therapeutic compound with deep purple color. In this work, we harnessed the modular and combinatorial feature of Golden Gate assembly method to construct library violacein producing strains in oleaginous yeast Yarrowia lipolytica, where each gene pathway was controlled by three different promoters varying transcriptional strength. After optimizing linker sequence reaction, achieved high transformation efficiency obtained panel representative Y....
Chalcone synthase (CHS) catalyzes the rate-limiting step of (2S)-naringenin (the essential flavonoid skeleton) biosynthesis. Improving activity CHS by protein engineering enhances production microbial fermentation and can facilitate valuable flavonoids. A biosensor based on TtgR operon was constructed in Escherichia coli its detection range expanded promoter optimization to 0–300 mg/L, widest for reported. The high-throughput screening scheme established this biosensor. mutant, SjCHS1S208N...
As the chip of synthetic biology, enzymes play a vital role in bio-manufacturing industry. The development diverse functional can provide rich toolbox for biology. This article reports construction an artificial enzyme with introduction non-natural cofactor. By introducing 4-dimethylaminopyridine (DMAP) cofactor into optimal protein skeleton via covalent bonds based on click-chemistry strategy, we successfully constructed novel DMAP as catalytic center. catalyzed unnatural asymmetric...
The emergence of photochromic fibers has created numerous opportunities in the realm intelligent textiles and functional materials. However, commercially accessible are predominantly produced from petroleum-based polymers, which contradicts current emphasis on sustainability minimizing carbon emissions. In this work, eco-friendly fast-reversible bio-based bacterial cellulose (BC) macrofibers that combined with 2,2,6,6-Tetramethylpiperidine-1-oxy (TEMPO) -oxidized BC (TOBC) nanofibers...
Enantiomerically pure (R, R)-2,3-butanediol has unique applications due to its special chiral group and spatial configuration. Currently, chemical production route many limitations. In addition, no native microorganisms can accumulate with an enantio-purity over 99%. Herein, we constructed a synthetic metabolic pathway for enantiomerically biosynthesis in Escherichia coli. The fermentation results suggested that introduction of the redistributed carbon fluxes neutral R)-2,3-butanediol, thus...
Pectin was considered as a potential candidate to improve the thermal stability of anthocyanins, and binding ability pectin anthocyanins influenced by its structure. In this study, sunflower pectins, modified ultrasound (40 kHz) for different periods time, were prepared used bind with extracted from purple sweet potato.
Consistent introduction of novel enzymes is required for developing efficient biocatalysts challenging biotransformations. Absorbing catalytic modes from organocatalysis may be fruitful designing new-to-nature with functions. Herein we report a newly designed artificial enzyme harboring pyrrolidine residue that catalyzes the asymmetric Michael addition cyclic ketones to nitroolefins through enamine activation high efficiency. Diverse chiral γ-nitro two stereocenters were efficiently prepared...
Abstract Violacein is a naturally occurring anticancer therapeutic with deep purple color. Yeast fermentation represents an alternative approach to efficiently manufacturing violacein from inexpensive feedstocks. In this work, we optimized the extraction protocol improve recovery ratio and purity yeast culture, including variations of organic solvents, choice mechanical shear stress, incubation time use cell wall-degrading enzymes. We also established quantitative correlation between HPLC...
Paenibacillus polymyxa is a potential strain for (R,R)-2,3-butanediol production. Here, we report an annotated draft genome sequence of P. ATCC 12321, which contains 4,429 protein-coding genes and 49 structural RNAs. This provides genetic basis better understanding the mechanism accumulation highly optically active (R,R)-2,3-butanediol.
Klebsiella pneumoniae CICC10011, a promising 2,3-butanediol producer, has received much attention because of its high productivity. Here, the first draft genome sequence this efficient strain may provide genetic basis for further insights into metabolic and regulatory mechanisms underlying production at titer.
Abstract Consistent introduction of novel enzymes is required for developing efficient biocatalysts challenging biotransformations. Absorbing catalytic modes from organocatalysis may be fruitful designing new‐to‐nature with functions. Herein we report a newly designed artificial enzyme harboring pyrrolidine residue that catalyzes the asymmetric Michael addition cyclic ketones to nitroolefins through enamine activation high efficiency. Diverse chiral γ‐nitro two stereocenters were efficiently...
Asymmetric Catalysis. The design and evolution of an enzyme for the asymmetric Michael addition cyclic ketones to nitroolefins by enamine catalysis is reported Zhi Zhou et al. in their Communication (e202404312).
The effects of lactic acid bacteria fermentation and in vitro simulated digestion on phenolic bioavailability, antioxidant activity purple sweet potato juice (PSPJ) were investigated. PSPJ was fermented by Lactobacillus rhamnosus Streptococcus thermophilus. viable bacterial count, components, activity, bioaccessibility, bioavailability analyzed during the process vitro. data displayed that increased total α-glucosidase inhibition, flavonoid content, ratephenolic content. activities improved...