A5083118166- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- Toxoplasma gondii Research Studies
- Heat shock proteins research
- Amino Acid Enzymes and Metabolism
- Retinoids in leukemia and cellular processes
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Genetic and Kidney Cyst Diseases
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Protein Structure and Dynamics
- Cell death mechanisms and regulation
- Hepatitis B Virus Studies
- Viral Infectious Diseases and Gene Expression in Insects
Karolinska Institutet
2019-2021
MRC Mitochondrial Biology Unit
2017
Medical Research Council
2017
Wellcome Trust
2017
Universitätsmedizin Göttingen
2014-2016
University of Göttingen
2008-2016
The inner membrane of mitochondria is especially protein rich and displays a unique morphology characterized by large invaginations, the mitochondrial cristae, boundary membrane, which in proximity to outer membrane. Mitochondrial proteins appear be not evenly distributed but instead organize into functionally distinct subcompartments. It unknown how organization achieved. We identified MINOS1/MIO10 (C1orf151/YCL057C-A), conserved protein. mio10-mutant yeast cells are affected growth on...
N-terminal targeting signals (presequences) direct proteins across the TOM complex in outer mitochondrial membrane and TIM23 inner membrane. Presequences provide directionality to transport process regulate machineries during translocation. However, surprisingly little is known about how presequence receptors interact with what role these interactions play preprotein transport. Here, we identify signal-binding sites of through photo-affinity labeling. Using engineered probes, photo...
Article29 April 2019Open Access Transparent process TEFM regulates both transcription elongation and RNA processing in mitochondria Shan Jiang orcid.org/0000-0003-2020-6447 Department of Medical Biochemistry Biophysics, Karolinska Institutet, Stockholm, Sweden Max Planck Institute Biology Ageing - Institutet Laboratory, Search for more papers by this author Camilla Koolmeister Jelena Misic Stefan Siira Harry Perkins Research Centre Research, The University Western Australia, Perth, WA,...
Two driving forces energize precursor translocation across the inner mitochondrial membrane. Although membrane potential (Δψ) is considered to drive of positively charged presequences through TIM23 complex (presequence translocase), activity Hsp70-powered import motor crucial for mature protein portion into matrix. In this study, we show that matrix proteins display surprisingly different dependencies on Δψ. However, a precursor's hypersensitivity reduction Δψ not linked respective...
Cancer cells depend on mitochondria to sustain their increased metabolic need and therefore constitute possible targets for cancer treatment. We recently developed small-molecule inhibitors of mitochondrial transcription (IMTs) that selectively impair gene expression. IMTs have potent antitumor properties in vitro vivo, without affecting normal tissues. Because therapy-induced resistance is a major constraint successful therapy, we investigated mechanisms conferring IMTs. employed...
ABSTRACT The intracellular protozoan Toxoplasma gondii induces persistent infections in various hosts and is an important opportunistic pathogen of humans with immature or deficient immune responses. ability to survive intracellularly largely depends on the blocking different proapoptotic signaling cascades its host cell. Fas/CD95 triggers apoptotic cascade that crucial for immunity outcome infectious diseases. We have determined mechanism by which T. counteracts death receptor-mediated cell...
Control of translation allows for rapid adaptation the cell to stimuli, rather than slower transcriptional control. We presume that translational control is an essential process in adipogenesis, especially first hours after hormonal stimulation. 3T3-L1 preadipocytes were cultured confluency and adipogenesis was induced by standard protocols using a cocktail. Cells harvested before 6 induction. mRNAs attached ribosomes (polysomal mRNAs) separated from unbound velocity sedimentation. Pools...
DOR/TP53INP2 acts both at the chromosomal level as a nuclear co-factor e.g. for thyroid hormone receptor and extrachromosomal an organizing factor of autophagosome. In previous study, DOR was shown to be down-regulated in skeletal muscle obese diabetic Zucker fa/fa rats.To identify sites differential expression metabolically active tissues, we measured differences white adipose tissue (WAT), brown (BAT), (SM) heart (HM) by qPCR. To assess whether is influenced short term nutritional factors,...