A5077788309- Fungal and yeast genetics research
- Cellular transport and secretion
- Plant nutrient uptake and metabolism
- Polyamine Metabolism and Applications
- Antifungal resistance and susceptibility
- Ubiquitin and proteasome pathways
- Spectroscopy Techniques in Biomedical and Chemical Research
- Plant Stress Responses and Tolerance
- 14-3-3 protein interactions
- Parasitic Infections and Diagnostics
- Photoacoustic and Ultrasonic Imaging
- Fungal Infections and Studies
- Gold and Silver Nanoparticles Synthesis and Applications
Czech Academy of Sciences
2016-2019
Czech Academy of Sciences, Institute of Physiology
2016-2019
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2019
Instituto de Biología Molecular y Celular de Plantas
2012-2016
Consejo Superior de Investigaciones Científicas
2013-2016
Universitat Politècnica de València
2012-2016
The intrinsic ability of cells to adapt a wide range environmental conditions is fundamental process required for survival. Potassium the most abundant cation in living and essential cellular processes, including regulation cell volume, pH protein synthesis. Yeast can grow from low micromolar molar potassium concentrations utilize sophisticated control mechanisms keep internal concentration viable range. We developed mathematical model Saccharomyces cerevisiae explore complex interplay...
The maintenance of ionic homeostasis is essential for cell viability, thus the activity plasma membrane ion transporters must be tightly controlled. Previous studies in Saccharomyces cerevisiae revealed that proper trafficking several nutrient permeases requires E3 ubiquitin ligase Rsp5 and, many cases, presence specific adaptor proteins needed substrate recognition. Among these are nine members arrestin-related (ART) family. We studied possible role ART family regulation monovalent cation...
The maintenance of potassium homeostasis is crucial for all types cells, including Candida glabrata. Three plasma-membrane systems mediating influx with different transport mechanisms have been described in yeasts: the Trk1 uniporter, Hak cation-proton symporter and Acu ATPase. C. glabrata genome contains only one gene encoding putative system uptake, uniporter. Therefore, its importance maintaining adequate levels intracellular appears to be critical cells. In this study, we first confirmed...
Abstract The absence of high-affinity potassium uptake in Candida glabrata , the consequence deletion TRK1 gene encoding sole potassium-specific transporter, has a pleiotropic effect. Here, we show that addition to changes basic physiological parameters (e.g., membrane potential and intracellular pH) decreased tolerance various cell stresses, loss high affinity also alters cell-surface properties, such as an increased hydrophobicity adherence capacity. efficient system results diminished...
Cell imaging heavily depends on fluorescent labels typically incompatible with Raman microscopy. The europium(iii) complex based dipicolinic acid (DPA) presented here is an exception from this rule. Although its luminescence bands are very narrow, their intensity comparable to the background bands. This makes it complementary less luminous compounds referred as tags. Through several examples we show that provides a morphological context in otherwise unstained cells, thus acting...