A5050138079- Olfactory and Sensory Function Studies
- Neurobiology and Insect Physiology Research
- Advanced Chemical Sensor Technologies
- RNA regulation and disease
- Photodynamic Therapy Research Studies
- Photoacoustic and Ultrasonic Imaging
- Nanoplatforms for cancer theranostics
- RNA and protein synthesis mechanisms
- Cell Image Analysis Techniques
- Virtual Reality Applications and Impacts
- Augmented Reality Applications
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Single-cell and spatial transcriptomics
- Neuroendocrine regulation and behavior
- Mosquito-borne diseases and control
- Biochemical Analysis and Sensing Techniques
- Photoreceptor and optogenetics research
- Viral Infections and Immunology Research
- Surgical Simulation and Training
- interferon and immune responses
Cold Spring Harbor Laboratory
2015-2025
Champalimaud Foundation
2015-2024
The central amygdala (CeA) has a key role in learning and expression of defensive responses. Recent studies indicate that somatostatin-expressing (SOM<sup>+</sup>) neurons the lateral division CeA (CeL) are essential for acquisition recall conditioned freezing behavior, which been used as an index response laboratory animals during Pavlovian fear conditioning. However, how exactly these participate conditioning whether they contribute to generation responses other than remain unknown. Here,...
While animals readily adjust their behavior to adapt relevant changes in the environment, neural pathways enabling these remain largely unknown. Here, using multiphoton imaging, we investigate whether feedback from piriform cortex olfactory bulb supports such behavioral flexibility. To this end, engage head-fixed male mice a multimodal rule-reversal task guided by and auditory cues. Both odor and, surprisingly, sound cues trigger responses cortical bulbar axons which precede report....
Recombinant Sindbis viruses are important tools in neuroscience because they combine rapid and high transgene expression with a capacity to carry large transgenes. Currently, two packaging systems based on the DH(26S)5'SIN DH-BB(tRNA;TE12) Defective Helper (DH) RNAs available for generating recombinant virus that is neurotropic (able infect neurons potentially other cells). Both produce fraction of viral particles can propagate beyond primary infected neuron. When injected into mouse brain,...
While animals readily adjust their behavior to adapt relevant changes in the environment, neural pathways enabling these remain largely unknown. Here, using multiphoton imaging, we investigated whether feedback from piriform cortex olfactory bulb supports such behavioral flexibility. To this end, engaged head-fixed mice a multimodal rule-reversal task guided by and auditory cues. Both odor and, surprisingly, sound cues triggered cortical bulbar responses which preceded report. Responses same...
Colorectal cancer (CRC) ranks among the most prevalent malignancies worldwide, driving a quest for comprehensive characterization methods. We report of ex vivo autofluorescence lifetime fingerprint colorectal tissues obtained from 73 patients that underwent surgical resection. specifically target characteristics collagens, reduced nicotine adenine (phosphate) dinucleotide (NAD(P)H), and flavins employing fiber-based dual excitation (375 nm 445 nm) optical imaging system....
Summary Neurons transmit information to distant brain regions via long-range axonal projections. In the mouse, area-to-area connections have only been systematically mapped using bulk labeling techniques, which obscure diverse projections of intermingled single neurons. Here we describe MAPseq (Multiplexed Analysis Projections by Sequencing), a technique that can map thousands or even millions neurons large sets with random RNA sequences ("barcodes"). Axons are filled barcode mRNA, each...
<title>Abstract</title> Colorectal cancer (CRC) ranks among the most prevalent malignancies worldwide, driving a quest for comprehensive characterization methods. We report of <italic>ex vivo</italic> autofluorescence lifetime fingerprint colorectal tissues obtained from 73 patients that underwent surgical resection. specifically target characteristics collagens, reduced nicotine adenine (phosphate) dinucleotide (NAD(P)H), and flavins employing fiber-based dual excitation (375 nm 445 nm)...
Abstract Recombinant Sindbis viruses are important tools in neuroscience because they combine rapid and high transgene expression with a capacity to carry large transgenes. Currently, two packaging systems based on the DH(26S)5’SIN DH-BB(tRNA;TE12) Defective Helper (DH) RNAs available for making recombinant virus that is neurotropic (able infect neurons potentially other cells). Both produce fraction of viral particles can propagate beyond primary infected neuron. When injected into mouse...