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home Acta Veterinaria Brno Journal of the University Veterinary Sciences Brno, Czech Republic Search:

The objective of this study was the detection tetracycline, chlortetracycline and oxytetracycline residues in raw cow's milk. When analysing bulk milk (n = 57) tanker trailer'a 113) samples, two methods were used simultaneously: a specific rapid test Milk Tetrasensor Kit high performance liquid chromatography (HPLC) with ultraviolet isocratic elution. For HPLC analysis, Breeze (Waters, USA), chromatographic system, used. samples underwent solid phase extraction before analysis. Nova Pack C8...

This study explores the biological validation of markers acute stress in pigs subjected to transportation for slaughter. The selected monitoring were neopterin and cortisol. Their levels pig serum measured two porcine syndrome genotypes, NN Nn, after a 30-min transport slaughterhouse. Blood samples withdrawn before (control group) immediately animals' arrival (experimental group). values cortisol 5.60+/-1.65 nmol/l 273.54+/-66.17 respectively. After transfer, concentration rose significantly...

The aim of this study was the determination lactoferrin in goat milk using HPLC method. Milk samples were collected at a farm South Moravia Region, Czech Republic. It established bulk tank raw (n = 24) and pasteurised (nn 27) that during lactation. Lactoferrin contents analysed by reverse phase high-performance liquid chromatography (RP-HPLC) with diode-array detector PDA 2996. Detection carried out wavelength 205 nm. average concentration 120 ± 18 μg/ml. content increasing within lactation...

Cholesterol concentrations in goat milk, milk cheeses, ewe's ewes dairy bioproducts, and of cholesterol, stigmasterol sitosterol butter, butter with added vegetable fats margarines were evaluated by RP HPLC method. Parallel analyses capillary GC performed. Prior to the final chromatographic saponification step was used, followed extraction unsaponificable residue into n-hexane. Parameters method compared parameters determination. The detection limits (LOD) determinated on bases blank samples...

The aim of the study was to investigate effects two modified atmospheres with a different combination gases on selected groups microorganisms and concentrations biogenic amines (BAs) in samples poultry breast muscle. were packaged under atmosphere A (75% O 2 25% CO ) or B N stored at temperatures from +2 +4 °C for 14 days. During storage period, (MA A) decreased initial 74.8 ± 0.3% 55.9 6.6% end period. In all samples, counts psychrotrophic bacteria counts, Brochothrix thermosphacta , lactic...

As far as honey is concerned, microwave oven heating finds its use especially for crystallised reliquefying. We focused on monitoring the changes in hydroxymethylfurfural content which an indicator of heat damage done to honey, among others. Microwave was carried out four degrees power levels over seven variously long time periods. In total, 22 analysed samples came directly from bee-keepers Czech Republic 2004 and 2006 harvests. Hydroxymethylfurfural determined by HPLC method using a liquid...

The aim of the study was to monitore levels fluoroquinolone residues in bulk samples raw cow's milk. milk (n = 150) were obtained from 58 different suppliers South Moravian and Vysočina Regions. analysed by reverse phase high-performance liquid chromatography method with fluorescence detection gradient elution. 87.3% positive for fluoroquinolones residues. Flumequine present none samples. other investigated below recommended maximum residue limit. results indicate that are frequently...

The aim of this study was to assess the quality raw cow’s milk from an automatic milking system. Samples (48) were analyzed chemically and microbiologically somatic cell count, freezing point inhibitor residues determined. For comparison purposes, analysis data two farms using conventional machine 2008 report for Czech Republic used. All physical chemical characteristics samples within established limits. average content (%) indicators following: fat 3.79 ± 0.18, protein 3.46 0.06, casein...

The aim of this study was to research the quality parameters honeys directly from Iraqi Kurdistan beekeepers and compare with European Union law Central honeys. In total 30 honey samples Kurdish, Czech Slovak were collected year production 2018. analyzed physicochemical bioactive compounds (moisture content, electrical conductivity, diastase activity, hydroxymethylfurfural pH, free acidity, phenolic color, color intensity) varied depending on botanical geographical origin Comparing a...

Using a model case of contamination long-life semi-skimmed milk with the spores six B. cereus strains, isolated from farm environment and raw milk, proteolysis was monitored by measuring changes in protein content infra-red spectroscopy; free tyrosine measured Lowry method according to Juffs, reduction casein fractions SDS-PAGE. Lipolysis dilution extractive method. At storage temperature 4 °C for months no enzyme processes were observed, whereas at 24 marked activity found during maximum 3...

The objective of this study was to determine protein, fat, lactose, total solids, non-fatty solids contents, freezing point, titratable acidity and pH using Fourier transform near infrared spectroscopy (FT-NIR). Sixty samples goat milk were used calibrate the instrument by partial least squares (PLS) method. spectra measured on integration sphere in reflectance mode with use a 0.1 mm wide transflectance cell. following statistical values obtained: correlation coefficient ( R ) = 0.920...

The aim of this study was to determine the actual content some exogenous contaminants in honey from South Moravian region Czech Republic. polycyclic aromatic hydrocarbons (PAHs) and risk elements (Mercury - Hg, Cadmium Cd, Lead Pb, Arsenic As) multifloral blossom dew samples were determined by high-performance liquid chromatography atomic absorption spectroscopy methods. collected form beekeepers retail stores located Moravia Concentrations individual PAHs ranged between 0.02 μg·kg -1 –1.93...

Protein analysis is very important both in terms of milk protein allergy, and dairy product adulteration (β-lactoglobulin may be an marker the detection adulteration). The aim this study was to detect major whey proteins α-lactalbumin β-lactoglobulin their genetic variants by reversed-phase high-performance liquid chromatography. Milk samples from cows (n = 40), goats 40) sheep were collected at two farms bars Czech Republic April June 2010. concentration higher goat’s (1.27 ± 0.05 g·l -1 ,...

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The freezing point (FP) was established in 48 bulk tank samples of raw and pasteurized goat milk that were collected the course lactation. Alongside, non-fat solids (NFS) content monitored. Milk measurements carried out using thermistor cryoscope method compliance with standard CTS 570538 (1998). mean found to be an interval -0.5513 ± 0.0046°C, variation ranged from -0.5466°C -0.5567°C, higher values spring months a drop at end FP corresponded NFS content. average heat-treated on farm...

In recent years, the popularity of goat's milk and products has been growing in Czech Republic, especially for its low allergenic potential good digestibility.This study focused on assessment microbiological quality safety raw heat-treated milk.During lactation period, total 48 samples 40 pasteurized were collected a farm South Moravian Region Republic.Quantitative analysis was performed to determine plate count (TPC) coagulase-positive (CP) staphylococci count.The presence E. coli including...

The study focused on the effects of traditional (benzoate-sorbate and triphosphates) alternative (nisin, Laktocid, Defence JB, Galimax Flavor) food preservatives microbiological quality sensory properties pasteurized liquid whole eggs (LWE). LWE samples with addition a test preservative control were stored at 4°C for 45 D. selected parameters, attributes, color space parameters determined. results statistically analyzed by means factorial ANOVA, followed Tukey post hoc test. multivariate...

The objective of this experiment was to monitor the possibility influencing composition milk and products by supplementing feed lactating goats with selenium in various forms (natrium selenite, proteinate). trial carried out 30 goats, divided into 3 groups: Group C (n = 10) a control group, SeI group received form natrium selenite SeO lactate-protein complex produced Lactobacillus acidophilus . Diverse supplementations were from 3.5–4 months before date delivery until 2nd month lactation. In...