Exposure of Neuro-2a and PC12 cells to micromolar concentrations sulfite caused an increase in reactive oxygen species a decrease ATP. Likewise, the biosynthesis ATP intact rat brain mitochondria from oxidation glutamate was inhibited by sulfite. Glutamate-driven respiration increased mitochondrial membrane potential (MMP), this abolished but MMP generated malate succinate not affected. The rate production NADH exogenous NAD+ added extracts sulfite, preincubated with failed reduce NAD+....

Akt, a serine/threonine kinase has been shown to stimulate glycolysis in cancer cells but its role mitochondrial respiration is unknown. Using PTEN-knockout mouse embryonic fibroblasts (MEFPTEN−/−) with hyper-activated Akt as cell model, we observed higher respiratory capacity MEFPTEN−/− compared the wildtype (MEFWT). The phenotype was reproduced MEFWT by gene silencing of PTEN which substantiated regulating function. increased activities complexes (RCs) I, III and IV were retained same...

In this study, we sought to better define the limit of spatial resolution at fingertips elderly participants (n = 30, age 60-95 years) using an extended set JVP grating domes, incorporating four new dimensions (2.5-, 3.5-, 4.0- and 4.5-mm width). A secondary aim was examine whether deficits in tactile acuity could be related hand dysfunction older adults. Spatial thresholds were determined by finest whose orientation (dominant index finger) reported reliably. Manual dexterity assessed with...

Glutathione (GSH), glutathione <i>S</i>-transferase (GST), and conjugate export pump (GS-X pump) have been shown to participate collectively in the detoxification of many anticancer drugs, including cisplatin. Identification regulation rate-limiting step overall system for cisplatin is crucial importance sensitization human tumor cells In this study, GSH content, GST activity, GS-X activity were regulated separately examine effects on cytotoxicity cisplatin-induced DNA interstrand...

ABSTRACT After weaning, the mammary gland ceases lactation and involutes. The wet weight of decreases by 70% within 4 days weaning. This involves significant tissue remodelling as ducts regress return to resting state. presence apoptotic bodies in luminal epithelial compartment 2 3 after weaning provides clear evidence that a substantial proportion regression is attributable induction active cell death (ACD) cells. These changes architecture were found be mirrored gene expression....

We have developed a novel library‐to‐library cross‐screening technology to clone unique mRNAs that are expressed during tissue regression. cloned number of regression selected genes (RSG) the mammary gland and ventral prostate rat after removal respective trophic hormone. In this investigation, we characterized one these genes, RSG‐2, is homologous cathepsin B, thiol protease has been previously identified as extracellular proteases which activated in metastatic cells. The steady‐state...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMicroheterogeneity of plasma albumin. VII. Investigation by the equilibrium salting-out method origins microheterogeneityKim Ping Wong and Joseph F. FosterCite this: Biochemistry 1969, 8, 10, 4104–4108Publication Date (Print):October 1, 1969Publication History Published online1 May 2002Published inissue 1 October 1969https://pubs.acs.org/doi/10.1021/bi00838a030https://doi.org/10.1021/bi00838a030research-articleACS PublicationsRequest reuse...

Intraperitoneal administration of vanillin (4-hydroxy-3-methoxybenzaldehyde) to rats gave rise a number urinary products, chief among which was vanillic acid, in both free and conjugated forms. Other metabolites were vanillin, vanillyl alcohol, catechol (pyrocatechol). Catechol continued be formed even after treatment the animals with neomycin streptomycin for 72 hours before administered. Besides following exogenous compounds shown sources catechol: protocatechuic aldehyde, guaiacol....

Several papers have claimed that mitochondria contain nitric oxide synthase (NOS) and make (NO•) in amounts sufficient to affect mitochondrial respiration. However, we found the addition of l-arginine or NOS inhibitor l-NMMA intact rat liver did not any effect on respiratory rate both State 3 4. We detect NO• production by oxymyoglobin oxidation assay, electrochemically using an electrode. An apparent detected Griess assay was identified as artifact. generated eNOS added could easily be...

Clonorchiasis may lead to cholangitis. Clonorchis sinensis infiltration of the biliary system produces an easily recognisable cholangiographic picture. The findings consist characteristic filling defects and changes in intrahepatic extrahepatic ducts. are usually small irregular size is uniform. change ducts consists ductal dilatation involving especially smaller ducts, apparent elongation, tortuosity duct wall irregularities. mainly irregularities a mild degree dilatation. recognition...