A5045629369- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- High Temperature Alloys and Creep
- Cancer-related Molecular Pathways
- Advanced Vision and Imaging
- Plant Molecular Biology Research
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Network Security and Intrusion Detection
- Single-cell and spatial transcriptomics
- Cancer-related gene regulation
- Early Childhood Education and Development
- Kruppel-like factors research
- Advanced Image Processing Techniques
- Additive Manufacturing Materials and Processes
- Cell Image Analysis Techniques
- Advanced optical system design
- Microfluidic and Bio-sensing Technologies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Intermetallics and Advanced Alloy Properties
- Image Processing Techniques and Applications
- Developmental Biology and Gene Regulation
- Protein Degradation and Inhibitors
- Advanced Materials Characterization Techniques
University of Michigan
2013-2024
National University of Defense Technology
2022-2024
Jinan University
2023-2024
University of Utah
2021-2022
Huntsman Cancer Institute
2021-2022
Michigan United
2019
Since the earliest observations of cells undergoing mitosis, it has been clear that there is an intimate relationship between cell cycle and nuclear chromatin architecture. The envelope undergo robust assembly disassembly during cycle, transcriptional post-transcriptional regulation histone biogenesis modification controlled in a cycle-dependent manner. Chromatin binding proteins modifications turn influence expression critical regulators, accessibility origins for DNA replication, repair,...
Specification of tissue identity during development requires precise coordination gene expression in both space and time. Spatially, master regulatory transcription factors are required to control tissue-specific programs. However, the mechanisms controlling how changes over time less well understood. Here, we show that hormone-induced temporal by regulating accessibility DNA elements. Using Drosophila wing, demonstrate accompanied genome-wide chromatin at temporal-specific enhancers. We...
During terminal differentiation, most cells exit the cell cycle and enter into a prolonged or permanent G0 in which they are refractory to mitogenic signals. Entry is usually initiated through repression of gene expression by formation transcriptional repressor complex called dimerization partner (DP), retinoblastoma (RB)-like, E2F MuvB (DREAM). However, when DREAM repressive function compromised during additional unknown mechanisms act stably repress cycling ensure robust exit. Here, we...
In animal cells, growth factor signaling promotes both cell cycle progression and growth, but the connection between these two processes is not well understood. Here, we investigated whether cycling are coupled through protein translation. Using ribosome profiling mRNA sequencing examined changes in translational activities human Retinal Pigment Epithelial (RPE-1) cells as they entered response to serum stimulation. We found that, addition mRNAs encoding factors biogenesis initiation, many...
Abstract The mechanisms that maintain a non-cycling status in postmitotic tissues are not well understood. Many cell cycle genes have promoters and enhancers remain accessible even when cells terminally differentiated state, suggesting their repression must be maintained long term. In contrast, enhancer decommissioning has been observed for rate-limiting the Drosophila wing, tissue where die soon after eclosion, but it unclear if this also occurs other contexts of terminal differentiation....
Genome organization changes during development as cells differentiate. Chromatin motion becomes increasingly constrained and heterochromatin clusters become restricted in their developmental potential. These coincide with slowing of the cell cycle, which can also influence chromatin dynamics. Terminal differentiation is often coupled permanent exit from existing data suggest a close relationship between repressive structure silencing cycle postmitotic cells. Heterochromatin clustering could...
Flow cytometry has been widely used to obtain information about DNA content in a population of cells, infer relative percentages different cell cycle phases. This technique successfully extended the mitotic tissues model organism Drosophila melanogaster for genetic studies regulation vivo. When coupled with cell-type specific fluorescent protein expression and manipulations, one can detailed effects on number, size phasing However this live-cell method relied use permeable Hoechst 33342...
Abstract The mechanisms that maintain a non-cycling status in postmitotic tissues are not well understood. Many cell cycle genes have promoters and enhancers remain accessible even when cells terminally differentiated state, suggesting their repression must be maintained long term. In contrast, enhancer decommissioning has been observed for rate-limiting the Drosophila wing, tissue where die soon after eclosion, but it unclear if this also occurs other contexts of terminal differentiation....
Using a gain-of-function screen in Drosophila, we identified the Krüppel-like factor Cabut (Cbt) as positive regulator of cell cycle gene expression and proliferation. Enforced cbt is sufficient to induce an extra division differentiating fly wing or eye, also promotes intestinal stem divisions adult gut. Although inappropriate proliferation results from forced E2f1 transcription its target, Cyclin E, Cbt does not increase E2F1 E activity. Instead, regulates large set target genes...
Fisheye imaging technique plays an important role in various areas. However, fisheye lenses can cause severe image distortion, which requires distortion rectification for these images. after rectification, the images will suffer from problem of resolution reduction, and degree reduction varies different regions. This directly affect subsequent process content analysis. Therefore, it is needed to adopt a super-resolution reconstruction algorithm enhance rectified image. paper designs...
In this investigation, we explore the impact of Nb on microstructural and mechanical properties high-entropy Superalloys (HESAs), focusing hierarchical microstructures. Utilizing a series HESAs with varying contents, our study employs advanced characterization techniques, including differential scanning calorimetry (DSC) for thermal analysis, electron probe micro-analyzer (EPMA) compositional analysis design homogenization treatment at 1,500 K/24 h. Transmission microscopy (TEM) reveals that...
Flow cytometry has been widely used to obtain information about DNA content in a population of cells, infer relative percentages different cell cycle phases. This technique successfully extended the mitotic tissues model organism Drosophila melanogaster for genetic studies regulation vivo. When coupled with cell-type specific fluorescent protein expression and manipulations, one can detailed effects on number, size phasing However this live-cell method relied use permeable Hoechst 33342...
During terminal differentiation most cells will exit the cell cycle and enter into a prolonged or permanent G0. Cell is usually initiated through repression of gene expression by formation transcriptional repressor complex called DREAM. However when DREAM repressive function compromised during differentiation, additional unknown mechanisms act to stably repress cycling ensure robust exit. Here we provide evidence that developmentally programmed, temporal changes in chromatin accessibility at...
Summary Background Genome organization changes during development as cells differentiate. Chromatin motion becomes increasingly constrained and heterochromatin clusters become restricted in their developmental potential. These coincide with slowing of the cell cycle, which can also influence chromatin dynamics. Terminal differentiation is often coupled permanent exit from cycle existing data suggests a close relationship between repressive structure silencing postmitotic cells. Here we...
In this paper, the existing Point Spread Function (PSF) estimation method is improved, and spatial variation PSF of aspherical single-lens imaging system estimated. Most methods for do not consider fusion adjacent regions, accuracy edge regions poor. a based on corner detection proposed, which makes full use similarity fuzzy kernels asphere lens symmetry non-curved PSF, improves with variation.