A5102737153- Advanced biosensing and bioanalysis techniques
- Graphene research and applications
- Graphene and Nanomaterials Applications
- Advanced Sensor and Energy Harvesting Materials
- Nanopore and Nanochannel Transport Studies
- Nanowire Synthesis and Applications
- Plasmonic and Surface Plasmon Research
- Gas Sensing Nanomaterials and Sensors
- Advanced Materials and Mechanics
- Analytical Chemistry and Sensors
- Microfluidic and Bio-sensing Technologies
- Molecular Junctions and Nanostructures
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- SARS-CoV-2 detection and testing
- Gold and Silver Nanoparticles Synthesis and Applications
- Metamaterials and Metasurfaces Applications
- Electrochemical Analysis and Applications
- Surface Modification and Superhydrophobicity
- Nanoparticle-Based Drug Delivery
- Iron Metabolism and Disorders
- MXene and MAX Phase Materials
- Advancements in Semiconductor Devices and Circuit Design
- Electrohydrodynamics and Fluid Dynamics
- Electrochemical sensors and biosensors
Gachon University
2021-2024
University of Illinois Urbana-Champaign
2018-2020
University of California, San Diego
2015-2018
La Jolla Bioengineering Institute
2017
Abstract Field-effect transistor (FET)-based biosensors allow label-free detection of biomolecules by measuring their intrinsic charges. The limit these sensors is determined the Debye screening charges from counter ions in solutions. Here, we use FETs with a deformed monolayer graphene channel for nucleic acids. These devices even millimeter scale channels show an ultra-high sensitivity buffer and human serum sample down to 600 zM 20 aM, respectively, which are ∼18 ∼600 acid molecules....
Circulating tumor cells (CTCs) contain molecular information on the primary and can be used for predictive cancer diagnostics. Capturing rare live CTCs their quantification in whole blood remain technically challenging. Here we report an aptamer-trigger clamped hybridization chain reaction (atcHCR) method situ identification subsequent cloaking/decloaking of by porous DNA hydrogels. These decloaked were then cell analysis. In our design, a staple strand with aptamer-toehold biblocks...
Significance We describe the first, to our knowledge, integrated dynamic DNA nanotechnology and 2D material electronics overcome current limitations for detection of single-nucleotide polymorphism (SNP). Electrical has been advancing rapidly achieve high specificity, sensitivity, portability. However, actual implementation is still in infancy because low especially analytically optimal practically useful length target strands. Most research date focused on enhancement sensitivity biosensors,...
Abstract Electronic DNA‐biosensor with a single nucleotide resolution capability is highly desirable for personalized medicine. However, existing DNA‐biosensors, especially polymorphism (SNP) detection systems, have poor sensitivity and specificity lack real‐time wireless data transmission. DNA‐tweezers graphene field effect transistor (FET) are used SNP transmitted wirelessly analysis. Picomolar of quantitative achieved by observing changes in Dirac point shift resistance change. The use...
Universal platforms for biomolecular analysis using label-free sensing modalities can address important diagnostic challenges. Electrical field effect-sensors are an class of devices that enable point-of-care by probing the charge in biological entities. Use crumpled graphene this application is especially promising. It previously reported limit detection (LoD) on electrical effect-based sensors DNA molecules FET (field-effect transistor) platform. Here, FET-based biosensing biomarkers...
The rapid and unexpected spread of SARS-CoV-2 worldwide has caused unprecedented disruption to daily life brought forward critical challenges for public health. disease was the largest cause death in United States early 2021. Likewise, COVID-19 pandemic highlighted need accurate diagnoses at scales larger than ever before. To improve availability current gold standard diagnostic testing methods, development point-of-care devices that can maintain sensitivity while reducing cost providing...
Abstract Enzymatic DNA amplification‐based approaches involving intercalating DNA‐binding fluorescent dyes and expensive optical detectors are the gold standard for nucleic acid detection. As components of a simplified miniaturized system, conventional silicon‐based ion sensitive field effect transistors (ISFETs) that measure decrease in pH due to generation pyrophosphates during amplification have been previously reported. In this article, Bst polymerase loop‐mediated isothermal (LAMP)...
Pharmaceuticals are generally designed to be nondegradable or slowly degradable prevent chemical degradation as it is employed therapeutics for human animal. This results in a widespread risk when they enter, accumulate persist the environment. Pharmaceutical pollution emerging wide-reaching concern due its ostensible consequences, by dissemination demands inventing novel analytical routes monitor and mitigate pharmaceutical pollutants. Therefore, this paper presents synthesis of Zinc...
Rapid, accurate, and label-free detection of biomolecules chemical substances remains a challenge in healthcare. Optical biosensors have been considered as biomedical diagnostic tools required numerous areas including the viruses, food monitoring, diagnosing pollutants environment, global personalized medicine, molecular diagnostics. In particular, broadly emerging promising technique surface plasmon resonance has established to provide real-time when used biosensing applications highly...
We propose surface plasmon resonance biosensors based on crumpled graphene and molybdenum disulphide (MoS 2 ) flakes supported stretchable polydimethylsiloxane (PDMS) or silicon substrates. Accumulation of specific biomarkers resulting in measurable shifts the wavelength modes two-dimensional (2D) material structures, with structures demonstrating large refractive index shifts. Using theoretical calculations semiclassical Drude model, combined finite element method, we demonstrate that...
We propose a novel, to the best of our knowledge, plasmonic-based methodology for purpose fast DNA sequencing. The interband surface plasmon resonance and field-enhancement properties graphene nanopore in presence nucleobases are investigated using hybrid quantum/classical method (HQCM), which employs time-dependent density functional theory quasistatic finite difference time domain approach. In strong plasmonic-molecular coupling regime where absorption frequencies degenerated, optical...
<title>Abstract</title> We present a nano-corrugation graphene (NCGr)-based device, which can support diverse detection strategies. A single NCGr device exhibit three different modes of biomolecular sensing: electrolyte-gated field-effect transistor (FET) sensing, electrochemical and sensing based on surface-enhanced Raman spectroscopy (SERS). Each mode produces reliable signals with extremely high sensitivity for DNA hybridization (analyte concentrations < 10 fM). The charge-transfer...
Nanocarriers with the ability to spatially organize chemically distinct multiple bioactive moieties will have wide combinatory therapeutic and diagnostic (theranostic) applications. We designed dual-functionalized, 100 nm 1 μm sized scalable nanocarriers comprising a silica golf ball amine or quaternary ammonium functional groups located in its pits hydroxyl on nonpit surface. These functionalized balls selectively captured 10-40 charged gold nanoparticles (GNPs) into their pits. The...
DNA can be manipulated to design nano-machines through specific sequence recognition. We report a switchable carrier for repeatable capture and release of single stranded DNA. The activity the was regulated by interactions among double-stranded actuator, target, fuel, anti-fuel strands. Inosine used maintain stable triple-stranded complex when actuator's conformation switched between open (capture) closed (release) configurations. Time lapse fluorescence measurements show target TEM images...
Current work in tuning DNA kinetics has focused on changing toehold lengths and concentrations. However, can also be improved by enhancing the completion probability of strand displacement process. Here, we execute this strategy creating a motor device with inclusion synthetic nucleotide, inosine, at selected sites. Furthermore, found that energetic bias tuned such stay stable partially displaced state. This demonstrates utility biases to change introduces complementary existing designs.
Plasmons in graphene present desirable electronic properties and unique opportunities to enhance light–matter interactions control light at nanoscale dimensions. The combination of with metal nanostructures is promising for optical science plasmonic manipulations. We propose a system based on resonant antenna detection counting nanoparticles. performance the proposed relies intrinsic particles, including refractive index, which can be used modulate transmitted power. Simulations show that,...
Real-time cell proliferation assays, incorporating fluorescent dyes into cells and bulky optical imaging systems, are the gold standard for cellular analysis at single-cell level. As components of a simplified miniaturized system, conventional field-effect transistor (FET)-based platform allows label-free detection biomolecules by measuring their inherent charge carriers. In this study, we developed winkled graphene-based FET (G-FET) to evaluate process with directional characteristics...