A5078567532- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Enzyme Structure and Function
- Gold and Silver Nanoparticles Synthesis and Applications
- Computational Drug Discovery Methods
- Plasmonic and Surface Plasmon Research
- Advanced Nanomaterials in Catalysis
- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- Nanocluster Synthesis and Applications
- Crystallography and molecular interactions
- vaccines and immunoinformatics approaches
- Crystallization and Solubility Studies
- Electrochemical sensors and biosensors
- T-cell and B-cell Immunology
- Graphene and Nanomaterials Applications
- X-ray Diffraction in Crystallography
- Protein purification and stability
- Bacteriophages and microbial interactions
- Nanopore and Nanochannel Transport Studies
Shanghai Institute of Applied Physics
2015-2024
Chinese Academy of Sciences
2015-2024
University of Chinese Academy of Sciences
2020-2024
Eli Lilly (United States)
2023
UCB Pharma (Belgium)
2013-2021
UCB Pharma (United Kingdom)
2012-2021
Berkshire Community College
2021
Renji Hospital
2020
Shanghai Advanced Research Institute
2020
Shanghai Jiao Tong University
2020
Abstract DNA is typically impermeable to the plasma membrane due its polyanionic nature. Interestingly, several different nanostructures can be readily taken up by cells in absence of transfection agents, which suggests new opportunities for constructing intelligent cargo delivery systems from these biocompatible, nonviral nanocarriers. However, underlying mechanism entry into remains unknown. Herein, we investigated endocytotic internalization and subsequent transport tetrahedral (TDNs)...
Structural antibody database (SAbDab; http://opig.stats.ox.ac.uk/webapps/sabdab) is an online resource containing all the publicly available structures annotated and presented in a consistent fashion. The data are with several properties including experimental information, gene details, correct heavy light chain pairings, antigen details and, where available, antibody-antigen binding affinity. user can select structures, according to these attributes as well structural such complementarity...
There remains a great challenge in the sensitive detection of microRNA because short length and low abundance microRNAs cells. Here, we have demonstrated an ultrasensitive platform for by combining tetrahedral DNA nanostructure probes hybridization chain reaction (HCR) amplification. The limits are 100 aM 10 (corresponding to 600 μL sample), respectively. Compared widely used supersandwich amplification, improved 3 orders magnitude. uncontrolled surface immobilization consumption target...
Abstract Self‐assembled DNA nanostructures with precise sizes allow a programmable “soft lithography” approach to engineer the interface of electrochemical sensors. By using millimeter‐sized gold electrodes modified several types tetrahedral (TDNs) different sizes, both kinetics and thermodynamics hybridization were profoundly affected. Because each probe is anchored on an individual TDN, its lateral spacing interactions are finely tuned by TDN size. simply varying size TDNs, time was...
Therapeutic mAbs must not only bind to their target but also be free from "developability issues" such as poor stability or high levels of aggregation. While small-molecule drug discovery benefits Lipinski's rule five guide the selection molecules with appropriate biophysical properties, there is currently no in silico analog for antibody design. Here, we model variable domain structures a large set post-phase-I clinical-stage therapeutics (CSTs) and calculate metrics estimate typical...
Abstract DNA‐based machines have attracted rapidly growing interest owing to their potential in drug delivery, biocomputing, and diagnostic applications. Herein, we report a type of exonuclease III (Exo III)‐powered stochastic DNA walker that can autonomously move on spherical nucleic acid (SNA)‐based 3D track. The motion is propelled by unidirectional Exo digestion hybridized tracks burnt‐bridge mechanism. operation this III‐propelled was monitored real time at the single‐particle...
Abstract In macromolecular crystallography, the rigorous detection of changed states (for example, ligand binding) is difficult unless signal strong. Ambiguous (‘weak’ or ‘noisy’) density experimentally common, since molecular are generally only fractionally present in crystal. Existing methodologies focus on generating maximally accurate maps whereby minor become discernible; practice, such map interpretation disappointingly subjective, time-consuming and methodologically unsound. Here we...
Graphene oxide (GO) is an excellent bacteria-killing nanomaterial. In this work, macroscopic applications of promising nanomaterial by fixing GO sheets onto cotton fabrics, which possess strong antibacterial property and great laundering durability, are reported. The GO-based fabrics prepared in three ways: direct adsorption, radiation-induced crosslinking, chemical crosslinking. Antibacterial tests show that all these GO-containing could inactivate 98% bacteria. Most significantly, can...
Computational modeling of antibody structures plays a critical role in therapeutic design. Several pipelines exist, but no freely available methods currently model nanobodies, provide estimates expected accuracy, or highlight potential issues with the antibody's experimental development. Here, we describe our automated pipeline, ABodyBuilder, designed to overcome these issues. The algorithm itself follows standard 4 steps template selection, orientation prediction,...
Circulating tumor cells (CTCs) contain molecular information on the primary and can be used for predictive cancer diagnostics. Capturing rare live CTCs their quantification in whole blood remain technically challenging. Here we report an aptamer-trigger clamped hybridization chain reaction (atcHCR) method situ identification subsequent cloaking/decloaking of by porous DNA hydrogels. These decloaked were then cell analysis. In our design, a staple strand with aptamer-toehold biblocks...
Uniform silver-containing metal nanostructures with strong and stable surface-enhanced Raman scattering (SERS) signals hold great promise for developing ultrasensitive probes biodetection. Nevertheless, the direct synthesis of such ready-to-use nanoprobes remains extremely challenging. Herein we report a DNA-mediated gold–silver nanomushroom interior nanogaps directly synthesized used multiplex simultaneous SERS detection various DNA RNA targets. The involved in can act as not only gap...
Mechanistic understanding of the endocytosis and intracellular trafficking nanoparticles is essential for designing smart theranostic carriers. Physico-chemical properties, including size, clustering surface chemistry regulate their cellular uptake transport. Significantly, even single could cluster intracellularly, yet state subsequent are not well understood. Here, we used DNA-decorated gold (fPlas-gold) as a dually emissive fluorescent plasmonic probe to examine states Evidence from...
Quantum computers can in principle solve certain problems exponentially more quickly than their classical counterparts. We have not yet reached the advent of useful quantum computation, but when we do, it will affect nearly all scientific disciplines. In this review, examine how current algorithms could revolutionize computational biology and bioinformatics. There are potential benefits across entire field, from ability to process vast amounts information run machine learning far...
Herein, we have developed a simple and facile method to synthesize yolk–shell nanostructured Fe3O4@C nanoparticles (NPs) as multifunctional biosensing platform for the label-free colorimetric detection of H2O2 glucose. It was demonstrated that nanostructures (YSNs) retained magnetic properties can be used separation concentration. Also importantly, YSNs exhibited an intrinsic peroxidase-like activity could quickly catalyze enzyme substrate in presence produce blue color. Compared other...
DNA nanorobots have emerged as new tools for nanomedicine with the potential to ameliorate delivery and anticancer efficacy of various drugs. nanostructures been considered one most promising nanocarriers. In present study, we report a framework-based intelligent nanorobot selective lysosomal degradation tumor-specific proteins on cancer cells. We site-specifically anchored an anti-HER2 aptamer (HApt) tetrahedral framework nucleic acid (tFNA). This (HApt-tFNA) could target HER2-positive...
Near-infrared (NIR)-II fluorescence agents hold great promise for deep-tissue photothermal therapy (PTT) of cancers, which nevertheless remains restricted by the inherent nonspecificity and toxicity PTT. In response to this challenge, we herein develop a hydrogen sulfide (H2S)-activatable nanostructured agent (Nano-PT) site-specific NIR-II fluorescence-guided PTT colorectal cancer (CRC). Our in vivo studies reveal that theranostic Nano-PT probe is specifically activated H2S-rich CRC tissues,...
Abstract DNA strand displacement reactions (SDRs) provide a set of intelligent toolboxes for developing molecular computation. Whereas SDR-based logic gate circuits have achieved high level complexity, the scale-up practical achievable computational tasks remains hurdle. Switching that were originally proposed by Shannon in 1938 and nowadays widely used telecommunication represent an alternative efficient means to realize fast-speed high-bandwidth communication. Here we develop switching...