A5017111649- Cardiac electrophysiology and arrhythmias
- Pluripotent Stem Cells Research
- Advanced biosensing and bioanalysis techniques
- Neuroscience and Neural Engineering
- Ion channel regulation and function
- Atrial Fibrillation Management and Outcomes
- Catalytic Processes in Materials Science
- Biosensors and Analytical Detection
- Epigenetics and DNA Methylation
- Nanomaterials for catalytic reactions
- Cardiac Arrhythmias and Treatments
- RNA Interference and Gene Delivery
- Cardiac pacing and defibrillation studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Magnetic Properties and Synthesis of Ferrites
- Coagulation and Flocculation Studies
- Genetics and Neurodevelopmental Disorders
- Extracellular vesicles in disease
- Metal and Thin Film Mechanics
- CRISPR and Genetic Engineering
- Insect and Arachnid Ecology and Behavior
- Supramolecular Self-Assembly in Materials
- Impact of AI and Big Data on Business and Society
- Materials Engineering and Processing
- Autophagy in Disease and Therapy
Shanghai Institute of Applied Physics
2016-2025
Chinese Academy of Sciences
2017-2025
Shanghai University
2016-2025
Zhengzhou Central Hospital
2023-2024
Henan Provincial People's Hospital
2023-2024
Ningbo Institute of Industrial Technology
2024
Tianjin University
2021-2024
Sichuan Agricultural University
2021-2024
Zhengzhou University
2023
German Centre for Cardiovascular Research
2018-2022
Uniform silver-containing metal nanostructures with strong and stable surface-enhanced Raman scattering (SERS) signals hold great promise for developing ultrasensitive probes biodetection. Nevertheless, the direct synthesis of such ready-to-use nanoprobes remains extremely challenging. Herein we report a DNA-mediated gold–silver nanomushroom interior nanogaps directly synthesized used multiplex simultaneous SERS detection various DNA RNA targets. The involved in can act as not only gap...
Abstract Severe infections like sepsis lead frequently to cardiomyopathy. The mechanisms are unclear and an optimal therapy for septic cardiomyopathy still lacks. aim of this study is establish endotoxin-induced inflammatory model using human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (hiPSC-CMs) mechanistic therapeutic studies. hiPSC-CMs were treated by lipopolysaccharide (LPS) in different concentrations times. ELISA, FACS, qPCR, patch-clamp techniques used the study....
Short QT syndrome (SQTS), a disorder associated with characteristic ECG QT-segment abbreviation, predisposes affected patients to sudden cardiac death. Despite some progress in assessing the organ-level pathophysiology and genetic changes of disorder, understanding human cellular phenotype discovering an optimal therapy has lagged because lack appropriate models disorder. The objective this study was establish model SQTS using human-induced pluripotent stem cell-derived cardiomyocytes...
Exosomes are potential biomarkers, which play an important role in early diagnosis and prognosis prediction of cancer-related diseases. Nevertheless, direct quantification exosomes biological fluid, especially point-of-care tests (POCTs), remains extremely challenging. Herein, we developed a sensitive portable electrochemical biosensor combination with smartphones for quantitative analysis exosomes. The improved double-antibody sandwich method-based poly-enzyme signal amplification was...
Background . Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are providing new possibilities for the biological study, cell therapies, and drug discovery. However, ion channel expression functions as well regulations in hiPSC-CMs still need to be fully characterized. Methods Cardiomyocytes were derived from hiPS cells that generated two healthy donors. qPCR patch clamp techniques used study. Results In addition reported channels, I Na , Ca-L Ca-T f NCX K1 Kr Ks KATP...
Our aim is to investigate the arrhythmogenic mechanism in right ventricular cardiomyopathy (ARVC)-patients by using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs).Human-induced were generated from human skin fibroblasts of two healthy donors and an ARVC-patient with a desmoglein-2 (DSG2) mutation. Patch clamp, quantitative polymerase chain reaction, calcium imaging techniques employed for study. The amplitude maximal upstroke velocity (Vmax) action potential (AP)...
A novel supramolecular DNA hydrogel system was designed based on a directly synthesized chemically branched DNA. For the formation, self-dimer with two sticky ends as linker to induce gelation of B-Y. By programing sequence, thermal and metal-ion responsiveness could be introduced into this system. This shows shear-thinning, designable responsiveness, good biocompatibility, which will simplify composition preparation process accelerate its biomedical applications.
DNA hydroxymethylation (5-hmC) is a kind of new epigenetic modification, which plays key roles in demethylation, genomic reprogramming, and the gene expression mammals. For further exploring functions 5-hmC, it necessary to develop sensitive selective methods for detecting 5-hmC. Herein, we developed novel multiplexing electrochemical (MEC) biosensor 5-hmC detection based on glycosylation modification enzymatic signal amplification. The was first glycosylated by T4 β-glucosyltransferase then...
Abstract Aims Brugada syndrome (BrS) is associated with a pronounced risk to develop sudden cardiac death (SCD). Up 21% of patients are related mutations in SCN5A. Studies identified SCN10A as contributor BrS. However, the investigation human cellular phenotype BrS presence remains lacking. The objective this study was establish model using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Methods and results Dermal fibroblasts obtained from patient suffering SCD...
Epigenetic alterations hold great promise as biomarkers for early stage cancer diagnosis. Nevertheless, direct identification of rare methylated DNA in the genome remains challenging. Here, we report an ultrasensitive framework nucleic acid-based electrochemical sensor quantitative and highly selective analysis methylation. Notably, can detect 160 fg million-fold unmethylated samples using this methylation-specific polymerase chain reaction (E-MSP) method. The high sensitivity E-MSP enables...
Biosensing interface based on screen-printed carbon electrodes (SPCE) has been widely used for electrochemical biosensors in the field of medical diagnostics, food safety, and environmental monitoring. Nevertheless, SPCE always a rough surface, which is easy to result disorder nucleic acid capture probes, nonspecific adsorption signaling steric hindrance target binding, decrease signal-to-noise ratio sensitivity biosensors. So far, it still remains extremely challenging develop...
Among rare channelopathies BrS patients are at high risk of sudden cardiac death (SCD). SCN5A mutations found in a quarter patients. Other gene including SCN1B have been implicated to BrS. Studying the human cellular phenotype associated with mutation remains lacking.We sought study variants using human-induced pluripotent stem cell (hiPSCs)-derived cardiomyocytes (hiPSC-CMs).A patient suffering from recurrent syncope harboring two (c.629T > C and c.637C A) SCN1B, which encodes...
Despite an increasing understanding of atrial fibrillation (AF) pathophysiology, translation into mechanism-based treatment options is lacking. In cardiomyocytes patients with chronic AF, expression, and function tandem P domains in a weak inward rectifying TASK-1 (K+ channel-related acid-sensitive K+ channel-1) (K2P3.1) atrial-specific 2-pore domain potassium channels enhanced, resulting action potential duration shortening. channel inhibition prevents shortening to maintain values observed...
5-hydroxymethylcytosine (5hmC) plays a pivotal role in the DNA demethylation pathway and transcriptional regulation. While sequencing-based methods such as TET-assisted bisulfite sequencing offer single-base resolution, they are not ideal for dynamic, time-sensitive quantification. Here, we present novel enzymatic biosensing strategy leveraging T7 endonuclease I rapid locus-specific 5hmC detection with resolution. This electrochemical platform captures double-tagged dsDNA detects by...
Short QT Syndrome (SQTS) is associated with sudden cardiac arrest (SCA). There limited data on the impact of antiarrhythmic drugs outcome SQTS. Material and Methods We studied describing clinical 62 SQTS patients treated drugs, recruited from a pool diagnosed in our institution also known databases after systematic search published literature. Results anti-arrhythmic were followed up over median timeframe 5.6 years (1.3-4.1 years). 6 particular, received multiple as combination. Out 55...
Abstract The non-selective cation channel transient receptor potential vanilloid 1 (TRPV1) is expressed throughout the cardiovascular system. Recent evidence shows a role for TRPV1 in inflammatory processes. of myocardial inflammation has not been established yet. Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (hiPSC-CM) from 4 healthy donors were incubated with lipopolysaccharides (LPS, 6 h), agonist capsaicin (CAP, 20 min) or antagonist capsazepine (CPZ, min). expression...
The cotton bollworm, Helicoverpa armigera (H. armigera), causes damage to a wide range of cultivated crops and is one the pests with greatest economic importance for global agriculture. Currently, detection H. based on manual sampling. A low limit (LOD), convenient, real-time monitoring method urgently needed its early warning efficient management. Here, we characterized amino acid sequence in sex pheromone receptors (SPRs) recognizing components by three-dimensional (3D) modeling molecular...
Abstract Recently, the coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has spread globally with major impact on public health. Novel methods that enable fast and efficient detection of virus associated biomarkers, such as SARS-CoV-2 antibodies, may provide alterative opportunities for early diagnosis, status monitoring, development vaccines. Here, we report fabrication a functionalized MoS -field effect transistor (FET) sensitive...
Aims: Some gene variants in the sodium channels, as well calcium have been associated with Brugada syndrome (BrS). However, investigation of human cellular phenotype and use drugs for BrS presence variant channel subunit is still lacking. Objectives: The objective this study was to establish a model CACNB2 uncertain significance (c.425C > T/p.S142F) using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) test drug effects model. Methods results: This recruited cells from...
The clinical significance of optimal pharmacotherapy for inherited arrhythmias such as short QT syndrome (SQTS) and long (LQTS) has been increasingly recognised. advancement gene technology opened up new possibilities identifying genetic variations investigating the pathophysiological roles mechanisms arrhythmias. Numerous variants in various genes have proven to be causative Studies demonstrated that effectiveness certain drugs is specific patient or genotype, indicating important role...
Natural products constitute and significantly impact many current anti-cancer medical interventions. A subset of natural induces injury processes in malignant cells that recruit activate host immune to produce an adaptive response, a process known as immunogenic cell death. However, challenge the field is delineate forms death best promote durable antitumor immunity. Addressing this with single-cell chemical biology product discovery platform, like multiplex activity metabolomics, would be...