A5114045332- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Ion channel regulation and function
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Data Mining Algorithms and Applications
- Bacteriophages and microbial interactions
- Nanopore and Nanochannel Transport Studies
- Data Management and Algorithms
- Modular Robots and Swarm Intelligence
- Machine Learning in Bioinformatics
- Peptidase Inhibition and Analysis
- Protein Structure and Dynamics
- Renal Diseases and Glomerulopathies
- Histone Deacetylase Inhibitors Research
- Computational Drug Discovery Methods
- Ubiquitin and proteasome pathways
- Click Chemistry and Applications
- Neuropeptides and Animal Physiology
- Gold and Silver Nanoparticles Synthesis and Applications
- Rough Sets and Fuzzy Logic
- Chronic Kidney Disease and Diabetes
East China Normal University
2016-2025
Chinese Academy of Sciences
2007-2016
Shanghai Institutes for Biological Sciences
2007-2016
Center for Excellence in Molecular Cell Science
2016
Chunghwa Telecom (Taiwan)
2011
Shanghai Institute of Materia Medica
2007
I-Shou University
2002
Protein-protein interactions (PPIs) are central to most biological processes. Although efforts have been devoted the development of methodology for predicting PPIs and protein interaction networks, application existing methods is limited because they need information about homology or marks partners. In present work, we propose a method PPI prediction using only sequences. This was developed based on learning algorithm-support vector machine combined with kernel function conjoint triad...
Specific and sensitive biomarker detection is essential to early cancer diagnosis. In this study, we demonstrate an ultrasensitive electrochemical biosensor with the ability detect multiple pancreatic carcinoma (PC)-related microRNA biomarkers. By employing DNA tetrahedral nanostructure capture probes enhance sensitivity as well a disposable 16-channel screen-printed gold electrode (SPGE) platform efficiency, were able simultaneously four PC-related miRNAs: miRNA21, miRNA155, miRNA196a,...
Abstract Protein-protein interactions are spatially regulated in living cells to realize high reaction efficiency, as seen naturally existing electron-transfer chains. Nevertheless, arrangement of chemical/biochemical components at the artificial device interfaces does not possess same level control. Here we report a tetrahedral DNA framework-enabled bulk enzyme heterojunction (BEH) strategy program multi-enzyme catalytic cascade interface electrochemical biosensors. The construction...
Understanding the behavior of biomolecules on nanointerface is critical in bioanalysis, which great challenge due to instability and difficulty control orientation loading density biomolecules. Here, we investigated thermodynamics kinetics DNA hybridization gold nanoparticle, with aim improve efficiency speed analysis. We achieved precise quantitative surface by applying a recently developed poly adenines (polyA)-based assembly strategy nanoparticles (DNA-AuNPs). PolyA served as an effective...
Abstract Weak ligand–receptor recognition events are often amplified by recruiting multiple regulatory biomolecules to the action site in biological systems. However, signal amplification vitro biomimetic systems generally lack spatiotemporal regulation vivo. Herein we report a framework nucleic acid (FNA)‐programmed strategy develop valence‐controlled amplifiers with high modularity for ultrasensitive biosensing. We demonstrated that FNA‐programmed could recruit acids, proteins, and...
The fixed dynamic range of traditional biosensors limits their utility in several real applications. For example, viral load monitoring requires the spans orders magnitude; whereas, drugs extremely narrow range. To overcome this limitation, here, we devised tunable biosensing interface using allosteric DNA tetrahedral bioprobes to tune biosensors. Our strategy takes advantage readily and flexible structure design predictable geometric reconfiguration nanotechnology. We reconfigured by...
ABSTRACT Two-pore domain potassium (K2P) channels gate primarily within the selectivity filter, termed ‘C-type’ gating. Due to lack of structural insights into nonconductive (closed) state, gating mechanisms remain elusive. Here, molecular dynamics (MD) simulations on TREK-1, a K2P channel, revealed that M4 helix movements induce filter closing in novel ‘deeper-down’ structure represents closed state. The ‘down’ does not represent state as previously proposed and instead acts an intermediate...
DNA nanostructures have attracted wide interest in biomedical applications, especially as nanocarriers for drug delivery. Therefore, it is important to ensure the structural integrity of under ambient temperature storage. In this study, we examined lyophilization-based preservation by investigating different reconstituted from lyophilization. We demonstrated that lyophilization appropriate ionic strength amenable storage nanostructures. Compared with stored liquid solution, nanostructure...
Despite advances in the development of direct KRAS inhibitors, KRAS-mutant cancers continue to exhibit resistance currently available therapies. Here, we identified REGγ as a mutant KRAS–associated factor that enhanced transcription through intermediate NRF2, suggesting REGγ-proteasome is potential target for pan-KRAS inhibitor development. We elucidated mechanism involving KRAS/NRF2/REGγ regulatory axis, which links activated ATP- and ubiquitin-independent proteasome. subsequently developed...
Abstract Weak ligand–receptor recognition events are often amplified by recruiting multiple regulatory biomolecules to the action site in biological systems. However, signal amplification vitro biomimetic systems generally lack spatiotemporal regulation vivo. Herein we report a framework nucleic acid (FNA)‐programmed strategy develop valence‐controlled amplifiers with high modularity for ultrasensitive biosensing. We demonstrated that FNA‐programmed could recruit acids, proteins, and...
The patient's response to drug treatment is usually systems-wide based on multi-spots through either direct or indirect targets. Thus, the evaluation of cannot rely single targeted biomarker, especially for complex diseases such as chronic kidney disease. In present study, we performed a analysis using proteomic approach quantify changes in profiles plasma from IgA nephropathy (IgAN) patients before and after treatment. particular, patient-to-health distances global proteome quantification...
Under the known pharmacological activation mechanisms, activators allosterically or directly open potassium channel gates. However, herein, molecular dynamics simulations on TREK-1, a member of class gated at filter, suggested that negatively charged act with gate-independent mechanism where compounds increase currents by promoting ions passing through central cavity. Then, based studies KCNQ2, we uncovered this noncanonical is shared other helix-bundle crossing. Rational drug design found...
Present a data mining method for discovering fuzzy functional dependencies from quantitative data. A number of algorithms crisp relational model have been proposed recently. The concept dependency is based on the assumption that are either fully dependent or independent. However, this too restrictive real-world applications. Our paper thus proposes to extract discovered contain not only conventional but also semantic between attributes. call be used in reconstruction conceptual structure...
Programmierte Gerüstnukleinsäuren (FNAs) sind Teil einer Strategie für die Entwicklung valenzkontrollierter Signalverstärker mit hoher Modularität hoch empfindliche Biosensorik. X. Zuo et al. demonstrieren in ihrer Zuschrift auf S. 7249, wie FNA-programmierten Nukleinsäuren, Proteine und anorganische Nanopartikel stöchiometrisch rekrutieren können. Die valenzkontrollierten ermöglichen den ultraempfindlichen Nachweis tumorrelevanter zirkulierender freier DNA (cfDNA).