A5077413713- Neural dynamics and brain function
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Visual perception and processing mechanisms
- Neuroscience and Neural Engineering
- Optical Coherence Tomography Applications
- Retinal Diseases and Treatments
- Advanced Fluorescence Microscopy Techniques
- Glaucoma and retinal disorders
- CRISPR and Genetic Engineering
- EEG and Brain-Computer Interfaces
- Receptor Mechanisms and Signaling
- Photochromic and Fluorescence Chemistry
- Neurobiology and Insect Physiology Research
- Retinal Imaging and Analysis
- Olfactory and Sensory Function Studies
- Connexins and lens biology
- Digital Imaging for Blood Diseases
- Cholesterol and Lipid Metabolism
- Axon Guidance and Neuronal Signaling
- Retinal and Macular Surgery
- interferon and immune responses
- Angiogenesis and VEGF in Cancer
- Ergonomics and Musculoskeletal Disorders
Polish Academy of Sciences
2014-2025
Institute of Physical Chemistry
2020-2025
International Institute for Molecular Oncology
2023-2024
Smith-Kettlewell Eye Research Institute
2024
University of California, Irvine
2018-2022
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2011-2020
Leber congenital amaurosis (LCA) is the most common cause of inherited retinal degeneration in children. LCA patients with RPE65 mutations show accelerated cone photoreceptor dysfunction and death, resulting early visual impairment. It therefore crucial to develop a robust therapy that not only compensates for lost function but also protects photoreceptors from further degeneration. Here, we vivo correction an Rpe65 mutation by adenine base editor (ABE) prolongs survival cones mouse model....
Aging, a universal process that affects all cells in an organism, is major risk factor for group of neuropathies called glaucoma, where elevated intraocular pressure one the known stresses affecting tissue. Our understanding molecular impact aging on response to stress retina very limited; therefore, we developed new mouse model approach this question experimentally. Here show susceptibility increases with age and primed chromatin level. We demonstrate ocular hypertension activates similar...
Adiponectin receptor 1 (ADIPOR1) is a lipid and glucose metabolism regulator that possesses intrinsic ceramidase activity. Mutations of the ADIPOR1 gene have been associated with nonsyndromic syndromic retinitis pigmentosa. Here, we show absence AdipoR1 in mice leads to progressive photoreceptor degeneration, significant reduction electroretinogram amplitudes, decreased retinoid content retina, reduced cone opsin expression. Single-cell RNA-Seq results indicate encoded most abundantly...
Inherited retinopathies are devastating diseases that in most cases lack treatment options. Disease-modifying therapies mitigate pathophysiology regardless of the underlying genetic lesion desirable due to diversity mutations found such diseases. We tested a systems pharmacology-based strategy suppresses intracellular cAMP and Ca2+ activity via G protein-coupled receptor (GPCR) modulation using tamsulosin, metoprolol, bromocriptine coadministration. The improves cone photoreceptor function...
Purpose.: Little is known about the physiological mechanisms underlying reported therapeutic effects of transorbital alternating current stimulation (ACS) in vision restoration, or origin recorded electrically evoked potentials (EEPs) during such stimulation. We examined issue EEP and electrode configuration for ACS characterized responses to CS different structures visual system. Methods.: visually (VEPs) EEPs from rat retina, thalamus, tectum, cortex. The VEPs were by light flashes...
To combat retinal degeneration, healthy fetal sheets have been successfully transplanted into both rodent models and humans, with synaptic connectivity between transplant degenerated host retina having confirmed. In studies, transplants shown to restore responses flashes of light in a region the superior colliculus corresponding location retina. determine quality detail visual information provided by transplant, responsivity was studied here at level cortex where higher perception is...
In this study, we used an inhibitor of phosphodiesterase 6 (PDE6) to examine the impact changes in conformation PDE6 protein on light-induced process responsible for altering length outer segments photoreceptor cells both human and rodent eyes. We employed a imaging method called spatiotemporal optical coherence tomography, which ensures high contrast phase stability within strongly scattering photoreceptor– Retinal Pigment Epithelium complex. Using approach, recorded nanometer-scale cones...
Noninvasive current stimulation (nCS or electrical stimulation) is a rapidly developing technique to support recovery from eye and brain dysfunctions. One of the most commonly used forms nCS for treating visual system transcranial transcorneal alternating stimulation. This can exert neuromodulatory effects on through The mechanism such still poorly understood. To understand pattern activation evoked by nCS, series impulses were delivered directly rat eye, with stimulus (VS), subsequent...
ABSTRACT The retina is uniquely enriched in polyunsaturated fatty acids (PUFAs), which are primarily localized cell membranes, where they govern membrane biophysical properties such as diffusion, permeability, domain formation, and curvature generation. During aging, alterations lipid metabolism lead to reduced content of very long-chain PUFAs (VLC-PUFAs) the retina, this decline associated with normal age-related visual pathological macular degeneration (AMD). ELOVL2 (Elongation...
Abstract Delivering ribonucleoproteins (RNPs) for in vivo genome editing is safer than using viruses encoding Cas9 and its respective guide RNA. However, transient RNP activity does not typically lead to optimal outcomes. Here we show that the efficiency of delivering RNPs can be enhanced by cell-penetrating peptides (covalently fused protein or as excipients) lipid nanoparticles (LNPs) encapsulating optimized stability, delivery potency. Specifically, after screening suitable ionizable...
The extrageniculate visual pathway, which carries information from the retina through superficial layers of superior colliculus and pulvinar, is poorly understood. pulvinar thought to modulate flow between cortical areas, has been implicated in cognitive tasks like directing visually guided actions. In order better understand underlying circuitry, we performed retrograde injections modified rabies virus cortex Long-Evans rat. We found a relatively small population cells projecting primary...
Microcirculation and neurovascular coupling are important parameters to study in neurological neuro-ophthalmic conditions. As the retina shares many similarities with cerebral cortex is optically accessible, a special focus directed assessing chorioretinal structure, microvasculature, hemodynamics of mice, vital animal model for vision neuroscience research.
Inherited retinal degenerative diseases are a prominent cause of blindness. Although mutations causing death photoreceptors mostly known, the pathophysiology downstream in inner retina and along visual pathway is incompletely characterized earliest disease stages. Here, we investigated retinal, midbrain cortical function using electroretinography (ERG), optomotor response (OMR), evoked potentials (VEPs), respectively, single unit electrophysiology at primary cortex (V1) light-adapted...
Mutations in many visual cycle enzymes photoreceptors and retinal pigment epithelium (RPE) cells can lead to the chronic accumulation of toxic retinoid byproducts, which poison underlying RPE if left unchecked. Without a functional ATP-binding cassette, sub-family A, member 4 (ABCA4), there is an elevation all- trans -retinal prolonged buildup adducts, resulting degenerative disease known as Stargardt-1 disease. Even this monogenic disorder, significant heterogeneity time onset symptoms...
Abstract Primary sensory areas of the mammalian neocortex have a remarkable degree plasticity, allowing neural circuits to adapt dynamic environments. However, little is known about effects traumatic brain injury on visual circuit function. Here we used anatomy and in vivo electrophysiological recordings adult mice quantify neuron responses stimuli two weeks three months after mild controlled cortical impact primary cortex (V1). We found that, although V1 remained largely intact...
Repetitive visual stimulation is successfully used in a study on the evoked potential (VEP) plasticity system mammals. Practicing tasks or repeated exposure to sensory stimuli can induce neuronal network changes cortical circuits and improve perception of these stimuli. However little known about effect training at subcortical level. In present study, we extend knowledge showing positive results this rat's Superior colliculus (SC). electrophysiological experiments, showed that single session...
Oscillations are ubiquitous features of neuronal activity in sensory systems and considered as a substrate for the integration information. Several studies have described oscillatory geniculate visual pathway, but little is known about this phenomenon extrageniculate pathway. We describe oscillations evoked background cat's superficial layers superior colliculus, retinorecipient structure Extracellular single-unit was recorded during periods with without stimulation under isoflurane...
Drifting gratings can modulate the activity of visual neurons at temporal frequency stimulus. In order to characterize modulation in cat's ascending tectofugal system, we recorded single superior colliculus, suprageniculate nucleus, and anterior ectosylvian cortex during stimulation with drifting sine-wave gratings. response such stimuli, each structure showed an increase firing rate and/or oscillatory modulated stimulus (phase sensitivity). To obtain a more complete characterization neural...
We report on a novel mice imaging system based the Spatio-Temporal Optical Coherence Tomography (STOC-T) technique. The contribution describes translation of STOC-T technique, initially developed for human eye imaging, into field experimental small animal imaging. present images retinal and choroidal tissue from B6 albino wild type mouse acquired at volumetric rate 112 Hz.
We present a novel ultrafast imaging system using Spatio-Temporal Optical Coherence Tomography (STOC-T), capable of acquiring structural images mouse retina at volumetric rate 112 Hz, aided by calibrated fundus camera for focal plane adjustment. extract blood pulse traces from retinal and choroidal vessels structural-only OCT analysis, wave-induced layer displacement differential phase analysis. With both analyses, we measure hemodynamic parameters, such as the delays between arterial venous...
We report on Spatio-Temporal Optical Coherence Tomography (STOC-T) applied to detection of pulsatile blood flow frequency, pulse wave propagation and other hemodynamic parameters from retinal choroidal vessels in the mouse eye.