A5046881902- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Histone Deacetylase Inhibitors Research
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Chemical Synthesis and Analysis
- Genetics and Neurodevelopmental Disorders
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Insect symbiosis and bacterial influences
University of Copenhagen
2013-2022
Novo Nordisk Foundation
2019
Embryonic development requires chromatin remodeling for dynamic regulation of gene expression patterns to ensure silencing pluripotent transcription factors and activation developmental regulators. Demethylation H3K27me3 by the histone demethylases Utx Jmjd3 is important lineage choice genes in response signals. To further understand function pluripotency differentiation we generated knockout embryonic stem cells (ESCs). Here show that not required proliferation ESCs, however, contributes...
The histone demethylase JMJD2C, also known as KDM4C/GASC1, has activity against methylated H3K9 and H3K36 is amplified and/or overexpressed in human cancers. By the generation of Jmjd2c knockout mice, we demonstrate that loss compatible with cellular proliferation, embryonic stem cell (ESC) self-renewal, development. Moreover, report JMJD2C localizes to H3K4me3-positive transcription start sites both primary cells carcinoma KYSE150 line containing an amplification locus. Binding dependent on...
Alteration of specific epigenetic marks might promote homology directed repair (HDR) during CRISPR-Cas9 genome editing. Testing several inhibitors in a traffic light reporter assay, the histone methylation inhibitor 3DZNep showed significant HDR promoting effect, while non-homologous end joining mediated was not significantly changed. This effect largely independent target gene and its expression levels but limited cell type specificity. promotion best described 3DZNep, H3K27...