A5088532546- NF-κB Signaling Pathways
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Skin and Cellular Biology Research
- Molecular Biology Techniques and Applications
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Cancer Cells and Metastasis
- Dermatology and Skin Diseases
- Melanoma and MAPK Pathways
- Cancer and Skin Lesions
- Wound Healing and Treatments
- Hedgehog Signaling Pathway Studies
- interferon and immune responses
- Genetic factors in colorectal cancer
- Hair Growth and Disorders
- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- Animal Genetics and Reproduction
- Cancer Research and Treatments
- CAR-T cell therapy research
- Kruppel-like factors research
- Genomics and Chromatin Dynamics
- Fibroblast Growth Factor Research
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas
2014-2024
Centro de Investigación Biomédica en Red de Cáncer
2017-2022
Research Institute Hospital 12 de Octubre
2016-2022
Technological University of the Mixteca
2021
Universidad de Alcalá
2019
Centre for Biomedical Network Research on Rare Diseases
2007-2011
Instituto de Biomedicina de Valencia
2011
Universidade de Santiago de Compostela
2011
Hospital Costa del Sol
2009
Instituto de Salud Carlos III
2007
UV radiation is the most important environmental skin aggressor, causing cancer and other problems. This paper reports use of oligonucleotide microarray technology to determine changes in gene expression human keratinocytes after UVB treatment. Examination effects different doses at times irradiation gave a global picture keratinocyte response this type insult. Five hundred thirty-nine regulated transcripts were found organized into nine clusters depending on behavior patterns....
Abstract Odontogenic tumours are a heterogeneous group of lesions that develop in the oral cavity region and characterized by formation tumoural structures differentiate as teeth. Due to diversity their histopathological characteristics clinical behaviour, classification these is still under debate. Alterations morphogenesis pathways such Hedgehog, MAPK WNT/β-catenin implicated odontogenic lesions, but molecular bases many unknown. In this study, we used genetically modified mice study role...
The suprabasal keratin 6 (K6) is remarkable among the keratins as, in addition to being constitutively expressed different stratified epithelia, it induced epidermis under hyperproliferative conditions, such as benign or malignant tumors, psoriasis, and wound healing. In addition, this also skin treated with 12-O-tetradecanoylphorbol-13-acetate retinoic acid (RA). These characteristics make study of K6 regulatory elements an especially interesting issue, particular because these could be...
The keratin 5 gene presents a complex regulation, since it is expressed at different rates in the basal cells of most, if not all, stratified epithelia.W e have analyzed 5'-upstream region bovine (BK5) and found that 5.2 kilobases preceding mimic vitro cell type-specific expression BK5. Most transcriptional activity maps toan enhancer located between positions -762 -1009.The only regulatory element this by electrophoretic mobility shift, competition, footprinting experiments consensus AP-1...
RAS genes are mutated in 20% of human tumors, but these mutations very rare breast cancer. Here, we used a mouse model to generate tumors upon activation mutagenic T2Onc2 transposon via expression transposase driven by the keratin K5 promoter p53
CYLD is a gene mutated in familial cylindromatosis and related diseases, leading to the development of skin appendages tumors. Although deubiquitinase tumor suppressor, its role physiology unknown. Using organotypic cultures as experimental model mimic human skin, we have found that acts regulator epidermal differentiation humans through JNK signaling pathway. We determined requirement for maintenance polarity, keratinocyte apoptosis. show overexpression increases while loss function impairs...
Abstract Tumor angiogenesis is governed by a complex balance of positive and negative angiogenic factors. Development chemically‐induced mouse skin tumors appears to be highly dependent on an early burst neovascularization. We have previously shown that Ha‐ ras –driven vascular endothelial growth factor (VEGF) expression plays pivotal role in this process. However, the status other critical factors throughout tumorigenesis has not been studied same extent. In present study, we show another...
p53 is a protein that causes cell cycle arrest, apoptosis or senescence, being crucial in the process of tumor suppression several types. Different vitro and animal models have been designed for study role skin cancer. These revealed opposing results, as some experimental settings it appears protects against cancer, but others, opposite conclusion emerges. We generated cohorts mice with efficient deletion restricted to stratified epithelia control littermates expressing wild type studied...
Abstract Differentiation‐related gene‐1 ( Drg‐1 ) has been identified as a gene whose expression is increased in several processes related to differentiation, but its function currently unknown. In this report, we show that was expressed keratinocytes, being rapidly result of induction by 12‐ O ‐tetradecanoylphorbol‐13‐acetate (TPA) or the presence an activating form Ha‐ras. Induction TPA occurred both cultured cell lines and primary keratinocytes well mouse skin after single application....
AbstractSquamous cell carcinomas (SCCs) of the skin display different clinical features according to their epithelial differentiation grade and histological variant. Understanding causes these divergences might increase curability SCCs. Therefore, it is important study mechanisms in keratinocytes. IKK (IκB kinase) α an protein for epidermal morphogenesis, although pathways through which exerts its function are unknown controversy exists about role cancer development. We show that enhanced...
Non-melanoma skin cancer is the most frequent type of in humans. In this study we demonstrate that elevated IKKα expression murine epidermis increases malignancy potential tumors. We describe generation transgenic mice overexpressing basal, proliferative layer and outer root sheath hair follicles. The K5-IKKα animals shows several alterations such as hyperproliferation, mislocalized integrin-α6 downregulation tumor suppressor maspin. Treatment back with mitogenic agent...
Alterations in nuclear factor kappaB (NFκB) signaling have been related with several diseases and importantly also cancer. Different animal models increased or diminished NFκB shown that subunits their regulators are relevant to the pathophysiology of different organs tissues. In particular, both deletion regulatory subunit β kinase inhibitor (IKKβ) its overexpression epidermis lead development skin inflammatory not associated tumoral lesions. this work, we studied consequences IKKβ other We...
IKKα plays a mandatory role in keratinocyte differentiation and exerts an important task non-melanoma skin cancer development. However, it is not fully understood how these functions. To analyze detail the of epidermal stratification differentiation, we have generated tridimensional (3D) cultures human HaCaT keratinocytes fibroblasts fibrin gels, obtaining equivalents that comprise dermal compartments resembles both structure normal skin. We found expression must be strictly regulated...
CYLD is a deubiquitinating enzyme known for its role as tumor suppressor whose mutation leads to skin appendages tumors and other cancers. In this manuscript we report that the CYLD, similarly renowned genes, protects from premature aging cancer. We have generated transgenic mice expressing mutant CYLDC/S protein, lacking deubiquitinase function, under control of keratin 5 promoter, K5-CYLDC/S mice. These express transgene in different organs, including those considered be more susceptible...
// Josefa P. Alameda 1, 2 , Miriam Gaspar 1 Ángel Ramírez Manuel Navarro Angustias Page Cristian Suárez-Cabrera M. Guadalupe Fernández 3 Jose R. Mérida Jesús Paramio Rosa A. García-Fernández 4 Fernández-Aceñero 5 Llanos Casanova Molecular Oncology Unit, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT), 28040 Madrid, Spain Oncology, Institute of Biomedical Investigation University Hospital "12 Octubre", 28041 Department Human Anatomy and Embriology, Facultad...