Background: Studies have shown that embryonic stem cells can differentiate into germ either spontaneously or directed by the defined factors applying a co-culture method conditioned medium of stromal cells. Objective: Here, Yazd4 (human [hESC] line; 46, XX) was used to evaluate effect human cumulus (CCCM) on in vitro cell production and development. Materials Methods: In this experimental study 2 different complete media were applied, Dulbecco’s modified eagle’s + 20% fetal bovin serum...

To investigate sperm chromatin/DNA integrity, global DNA methylation, and DNMT mRNA transcription in men with oligoasthenoteratozoospermia (OAT) compared normozoospermic men.Semen samples from 32 OAT patients who comprised the case group control were isolated purified using a standard gradient isolation procedure according to World Health Organization criteria. DNMT1, DNMT3A, DNMT3B transcripts then between groups real-time quantitative reverse-transcription polymerase chain reaction. Global...

FADS1 rs174556 polymorphism influences on dietary fats metabolism and type 2 diabetes (T2DM). This study aimed to compare the effect of three oils sesame, canola sesame-canola cardio metabolic factors across genotypes variant in patients with diabetes. was a randomized triple-blind three-way cross-over clinical trial. 95 Subjects T2DM replaced their regular oil sesame oil, or for 9-week phases completed study. There were anthropometric measurements, blood sampling biochemical assessments at...

Abstract Considerable advances have been made in identification of the involvement immune modulators diseases. There is growing evidence on role complement pathway pathogenesis and course multiple sclerosis (MS). Moreover, it has recognized that microRNAs (miRNAs) play an essential modulation development response central nervous system. We aimed to investigate expression profile factor H (CFH) miR‐146a genes experimental autoimmune encephalomyelitis (EAE) mouse model MS detect possible roles...

Cumulus cells, as oocyte nurse provide a suitable microenvironment with growth factors and cellular interactions required for maturation. Thus, these cells may serve natural niche in vitro studies of female germ cell development. help attain better understanding the causes infertility women eventually improve outcomes cases that respond poorly to standard treatment.The aim this study was isolate, culture, investigate biological characteristics human cumulus cells.In experimental study, were...

Introduction: Cholesteryl ester transfer protein (CETP) is a key regulating enzyme in the lipid metabolism pathway, and its gene polymorphism may be candidate for modulating metabolic responses to dietary intervention. We thus examined whether effects of CETP TaqIB on profiles were modified by plant oils. Methods: This retrospective analysis data collected during randomized triple-blind cross over trial. A total 95 patients with type 2 diabetes 73 non-diabetes individuals completed 9-weekof...

Process of angiogenesis is essential for successful gestation. Disruption in this pathway leads to various undesirable consequences pregnancy such as recurrent spontaneous abortion (RSA). One the most important genes involved kinase-insert domain-containing receptor (KDR). This study aimed investigate associations between two single-nucleotide polymorphisms (SNPs) KDR gene, 1719A > T and 1192G A, with idiopathic RSA south-east Iran.A total 230 women, including 110 women a history at least...

Background: Cell banking initial outgrowths from newly derived human embryonic stem cells (hESCs) requires an efficient freezing method. Vitrification is used for the preservation of gametes and early embryos in assisted reproduction techniques (ART). Moreover, vitrification was applied cryopreservation hESCs using open pulled straws. Objective: To derive characterize new hESC lines then use Cryotech Cryowin tools their vitrification. Materials Methods: Human ESC were generated a microdrop...

Noise-induced hearing loss is one of the most common occupational diseases in industrialized countries and can be affected by various environmental genetic factors. This study was designed to examine effect myricetin preventing this disorder.Twenty-one Wistar rats were randomly divided into five groups: Non-exposed, noise exposure only, with vehicle, 5 mg/Kg, 10 mg/kg. All animals sacrificed after last exposure. The left cochlea dissected from each rat. It used for mRNA expression analysis...

Human endometrium with consecutive regeneration capability undergoes monthly hormonal changes for probable implantation, which confirms the presence of cells in basalis layer known as stem cell.Previously, we reported isolation and culture mesenchymal-like from human endometrium. In this study, evaluated biological stemness characteristics these cells.The characterization Yazd endometrial-derived mesenchymal stem/stromal (YhEnMSCs) was assessed using immunofluorescence (IF) staining CD105,...

This study aimed to evaluate the relationship between mRNA expression of DNA methyltransferases (DNMTs) such as DNMT1, DNMT3A and DNMT3B sperm global methylation with protamine transcripts in from men severe abnormalities. Sperm each semen sample were isolated using a standard gradient isolation procedure by layering 1 mL 40% (v/v) density medium over 80% (v/v). A total 30 oligoasthenoteratozoospermic ejaculates (OAT) normozoospermic controls compared real-time quantitative reverse...

Androgens play a role in the development of male phenotype and spermatogenesis during puberty, function which is regulated by androgen receptor (AR) gene. There polymorphism site exon 1 gene encoding this that can have different frequencies CAG trinucleotide repeats leads to formation polyglutamine chains lengths N-terminal domain AR protein reduced sperm production affecting spermatogenesis.To investigate whether cause group unexplained infertilities could be increased frequency patients...

This report explains briefly the minutes of a 1-day workshop entitled; "human embryonic stem cells (hESCs) and good manufacturing practice (GMP)" held by Stem Cell Biology Research Center based in Yazd Reproductive Sciences Institute at Shahid Sadoughi University Medical Sciences, Yazd, Iran on 27

Background: Fibroblasts from different parts of the human body have been used in cell biology, drug discovery and therapy studies. One most available sources fibroblasts is neonatal foreskin. Not only do these cells woundhealing applications, but they are also popular source for pluripotent stem biotechnology. Moreover, several studies indicated that display similar features to mesenchymal cells. Objective: Generation establishment new foreskin fibroblast lines called Yazd (YhFFs). Materials...

BACKGROUND Previous studies demonstrated the critical role of miRNAs in carcinogenesis. Aberrant expression miR-127-3p and miR-144-3p have been revealed several types cancers. METHODS Expression levels were detected plasma patients with gastric cancer (GC) using fluorescent quantitative polymerase chain reaction to recognize potential non-invasive biomarkers for GC evaluated relationship between their clinicopathological parameters GC. RESULTS The results showed significantly decreased (p =...