A5007140449- Bladder and Urothelial Cancer Treatments
- Inhalation and Respiratory Drug Delivery
- Prostate Cancer Treatment and Research
- Cancer Cells and Metastasis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Epigenetics and DNA Methylation
- Ion Channels and Receptors
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- 3D Printing in Biomedical Research
- Ion channel regulation and function
- Innovative Microfluidic and Catalytic Techniques Innovation
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- Intracerebral and Subarachnoid Hemorrhage Research
- Liver physiology and pathology
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Pulmonary Hypertension Research and Treatments
- Neurosurgical Procedures and Complications
- Bioactive Compounds and Antitumor Agents
- Antimicrobial Peptides and Activities
- thermodynamics and calorimetric analyses
- Estrogen and related hormone effects
University of Bern
2019-2023
University Hospital of Bern
2021-2022
Swisscom (Switzerland)
2021
University Hospital of Lausanne
1982
Abstract Therapy resistance and metastatic processes in prostate cancer (PCa) remain undefined, due to lack of experimental models that mimic different disease stages. We describe an androgen-dependent PCa patient-derived xenograft (PDX) model from treatment-naïve, soft tissue metastasis (PNPCa). RNA whole-exome sequencing the PDX organoids confirmed transcriptomic genomic similarity primary tumor. PNPCa harbors BRCA2 CHD1 somatic mutations, shows SPOP/FOXA1 -like signature microsatellite...
Bladder Cancer (BLCa) inter-patient heterogeneity is the primary cause of treatment failure, suggesting that patients could benefit from a more personalized approach. Patient-derived organoids (PDOs) have been successfully used as functional model for predicting drug response in different cancers. In our study, we establish PDO cultures BLCa stages and grades. PDOs preserve histological molecular parental tumors, including their multiclonal genetic landscapes, consistently share key...
The evaluation of inhalation toxicity, drug safety and efficacy assessment, as well the investigation complex disease pathomechanisms, are increasingly relying on in vitro lung models. This is due to progressive shift towards human-based systems for more predictive translational research. While several cellular models currently available upper airways, modelling distal alveolar region poses constraints that make standardization reliable relatively difficult. In this work, we present a new...
Despite latest advances in prostate cancer (PCa) therapy, PCa remains the third-leading cause of cancer-related death European men. Dysregulation microRNAs (miRNAs), small non-coding RNA molecules with gene expression regulatory function, has been reported all types epithelial and haematological cancers. In particular, miR-221-5p alterations have PCa. miRNA data was retrieved from a comprehensive publicly available dataset 218 patients (GSE21036) levels were analysed. The functional role...
Transient receptor potential melastatin 4 (TRPM4) is a broadly expressed Ca2+ activated monovalent cation channel that contributes to the pathophysiology of several diseases. For this study, we generated stable CRISPR/Cas9 TRPM4 knockout (K.O.) cells from human prostate cancer cell line DU145 and analyzed for changes in hallmark functions. Both TRPM4-K.O. clones demonstrated lower proliferation viability compared parental cells. Migration was also impaired Additionally, analysis 210 patient...
Cathelicidins encompass a family of cationic peptides characterized by antimicrobial activity and other functions, such as the ability to enhance sensing nucleic acids innate immune system. The present study aimed investigate bovine cathelicidins indolicidin, bactenecin (Bac)1, Bac5, myeloid peptide (BMAP)-27, BMAP-28, BMAP-34 inhibit growth bacteria acid host's BMAP-27 was most effective at killing Staphylococcus aureus, Streptococcus uberis, Escherichia coli, this dependent on its...
Abstract Bladder Cancer (BLCa) inter-patient heterogeneity is considered the primary cause of tumor reoccurrence and treatment failure, suggesting that BLCa patients could benefit from a more personalized approach. Patient-derived organoids (PDOs) have been successfully used as functional model for predicting drug response in different cancer types. In our study, we established PDO cultures stages. PDOs preserve histological molecular parental tumors, including their multiclonal genetic...
Abstract Therapy resistance and metastatic processes in prostate cancer (PCa) remain undefined, due to lack of experimental models that mimic different disease stages. We describe a novel androgen-dependent PCa patient-derived xenograft (PDX) model from treatment-naïve, soft tissue metastasis (PNPCa). RNA whole-exome sequencing the PDX organoids confirmed transcriptomic genomic similarity primary tumor. PNPCa harbours BRCA2 CHD1 somatic mutations, shows an SPOP/FOXA1 -like signature...
Idiopathic Pulmonary fibrosis (IPF) is an incurable chronic disease characterized by progressive loss of lung function. IPF thought to occur due repetitive micro injuries in the distal and/or genetic predisposition leading aberrant tissue repair. Increased ECM deposition myofibroblasts results stiffening and thickening alveolar walls, causing decline pulmonary function ultimately, fatal respiratory failure. Due inherent complexity human disease, advanced vitro models including physiological...
COVID-19 is an infectious disease caused by the newly discovered coronavirus named SARS-CoV-2. The virus enters body through airways exploiting angiotensin-converting enzyme 2 (ACE2) and serine proteinase TMPRSS2. Thus, especially lung epithelial cells are attacked virus. In distal lung, infection leads to life-threatening alveolar damage cytokine storm. Many excellent clinical studies described pathology of progression in patients. However, impactful <italic>in vitro</italic> still missing...
Abstract Bladder Cancer (BLCa) inter-patient heterogeneity is the primary cause of treatment failure, suggesting that patients could benefit from a more personalized approach. Patient-derived organoids (PDOs) have been successfully used as functional model for predicting drug response in different cancers. In our study, we established PDO cultures BLCa stages. PDOs preserve histological and molecular parental tumors (PT), including their multiclonal genetic landscapes, consistently share key...
Abstract Introduction: Interpatient heterogeneity is one of the causes treatment failure in bladder cancer (BLCa) patients, who would therefore profit from tailor-made therapies. Toward direction precision medicine, patient-derived organoids (PDOs), which retain parental tumor (PT) features, have been successfully tested different types and shown to predict patients’ responses. Therefore, our study aimed explore ability BLCa PDOs PT features determine patient drug sensitivity. We correlated...
You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology IV (PD47)1 Apr 2020PD47-12 CHACTERISATION OF BLADDER CANCER DRUG RESPONSES IN ORGANOIDS AND EX VIVO MODELS Mirjam Kiener, Sofia Karkampouna, Federico La Manna, Marta De Menna, George N. Thalmann*, Roland Seiler-Blarer, and Marianna Kruithof-de Julio KienerMirjam Kiener More articles by this author , KarkampounaSofia Karkampouna MannaFederico Manna MennaMarta Menna Thalmann*George Thalmann* Seiler-BlarerRoland...
Abstract Therapy resistance and metastatic processes in prostate cancer (PCa) remain undefined, mainly due to the lack of experimental models representing different disease stages. We aim provide functional preclinical drug tools applicable for direct use with patient-derived material. Biopsies from PCa were used establishment xenografts (PDXs) by subcutaneous implantation. In vivo tumor growth kinetic response androgens was assessed surgical castration testosterone supplementation. RNA...
You have accessJournal of UrologyCME1 May 2022MP06-07 APPLICATION OF BLADDER CANCER PATIENT-DERIVED ORGANOIDS AS PRE-CLINICAL MODEL FOR PERSONALIZED MEDICINE Martina Minoli, Mirjam Kiener, Thomas Cantore, Tarcisio Fedrizzi, Paola Gasparini, Francesca Demichelis, George N. Thalmann, Roland Seiler-Blarer, and Marianna Kruithof-de Julio MinoliMartina Minoli More articles by this author , KienerMirjam Kiener CantoreThomas Cantore FedrizziTarcisio Fedrizzi GaspariniPaola Gasparini...
You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology III (MP66)1 Sep 2021MP66-17 BLADDER CANCER: PATIENT-DERIVED ORGANOIDS AS A TOOL FOR PRECISION MEDICINE Martina Minoli, Mirjam Kiener, Tarcisio Fedrizzi, Paola Gasperini, Francesca Demichelis, George Niklaus Thalmann, Roland Seiler-Blarer, and Marianna Kruithof-de Julio MinoliMartina Minoli More articles by this author , KienerMirjam Kiener FedrizziTarcisio Fedrizzi GasperiniPaola Gasperini DemichelisFrancesca...