Triage in emergency departments (EDs) is a dynamic decision-making process to prioritize patients based on their medical care needs. The Emergency Severity Index (ESI) simple-to-use, five-level triage system that categorizes ED according clinical urgency. nurse's ability obtain brief history and rapidly assess urgency crucial for ensuring safe efficient care. This study aimed the of registered nurses accurately identify pediatric classified as Priority 1 (P1) 2 (P2) under ESI, timely,...

Silver nanoparticles (AgNPs) have several applications in nanoscience and nanomedicine such as antibacterial, antifungal, anti-inflammatory anti-angiogenic properties. In the present study, AgNPs were synthesized by chemical method characterised using a combination of UV-Visible (UV-Vis) spectrophotometry, Fourier Transform Infra-Red (FTIR) spectroscopy, transmission electron microscopy (TEM) X-ray diffraction (XRD). The made chemically reducing AgNO3 with NaBH4 leading to formation...

Quercetin is a widely used bioflavonoid found in onions, grapes, berries and citrus fruits. Under certain conditions, quercetin acts as pro-oxidant thereby generating reactive oxygen species promoting the oxidation of molecules. Our study investigates effect on structure function alpha-2-macroglobulin (α2M) by employing various biophysical techniques trypsin inhibitory assay. α2M major antiproteinase present plasma vertebrates. Results activity assay indicated that loses its 56% treatment...

Organophosphate class of pesticides causes neurotoxicity and carcinogenicity in humans. Once inside the human body, these often interact with plasma proteins, such as alpha-2-macroglobulin (α2M) which is key anti-proteinase. Our work focuses on structural functional alteration α2M by chlorpyrifos (CPF), a member organophosphates. We explored binding interaction between CPF using UV absorption fluorescence spectroscopy (steady state synchronous), circular dichroism molecular docking approach....

Bilirubin is an endogenous antioxidant that a metabolite of the heme in red blood cells (RBC). In blood, bilirubin associated with albumin to form water-soluble complex, known as unconjugated bilirubin. Alpha-2-macroglobulin (α2M) proteinase inhibitor found plasma vertebrates. present study, we have investigated interaction photo-illuminated serum α2M using various biophysical and thermodynamic techniques. The binding leads functional structural changes protein. result ultraviolet (UV)...

Ifosfamide is an active alkylating chemotherapeutic drug chemically related to nitrogen mustard. The pharmacokinetics of drugs affected upon binding with protein, making the studies on drug-protein interaction promising. present study investigates between ifosfamide and human antiproteinase-alpha-2-macroglobulin (α2M) by using multi-spectroscopic in silico techniques. UV-visible absorption, intrinsic fluorescence circular dichroism (CD) spectroscopic methods were employed unveil mode...

Background: Silver nanoparticles (AgNPs) have a broad spectrum of applications in nanoscience and nanomedicine due to their flexible properties, such as antibacterial, antifungal, anti-inflammatory anti-angiogenic. Present study investigated the interaction chemically synthesized AgNPs with human major antiproteinase alpha-2-macroglobulin (α2M).Materials methods: The first step involved synthesis characterization using various biochemical biophysical techniques, UV-visible spectroscopy,...

Background: Aldicarb is a carbamate pesticide commercially used in potato crop production. Once it enters human body, interacts with diverse proteins and other substances. Objective: toxic to health also cholinesterase inhibitor, which prevents the breakdown of acetylcholine synapse. Human alpha-2-macroglobulin (α2M), large tetrameric glycoprotein 720 kDa antiproteinase activity, found abundantly plasma. Methods: In present study, interaction aldicarb was explored utilizing various...

5-Fluorouracil (5-FU) is a well-recognized anticancer drug used in the treatment of tumors head, neck and breast. Drug pharmacokinetics affected upon binding with protein, thus, making drug–protein interactions imperative to study. Present work investigates interaction between 5-FU human major antiproteinase-alpha-2-macroglobulin (α2M) by multi-spectroscopic, calorimetric molecular docking techniques. UV/Visible absorption, intrinsic fluorescence circular dichroism (CD) spectroscopic methods...

Background: Near precise weight assessment among sick pediatric patients remains a dilemma and conventionally accepted methods, in busy ED might be inaccurate or unreliable. Children have different weights at ages, accurate/precise measurement is of utmost importance for weight-related dose calculation drugs/fluids, equipment sizes, an accurate electrical currents during cardiac shock, etc. Several estimation methods are available dependent on the child's age, length, both. However, length...