We have optimized the design and imaging procedures, to clearly resolve malaria parasite in Giemsa-stained thin blood smears, using simple low-cost cellphone-based microscopy with oil immersion. The microscope uses a glass ball as objective phone camera tube lens. Our optimization includes optimal choice of lens diameter, size position aperture diaphragm, proper application immersion, achieve diagnostic capacity wide field view. resulting system is potentially applicable in-the-field optical...

Introduction Schistosomiasis is a significant public health concern, especially in Sub-Saharan Africa. Conventional microscopy the standard diagnostic method resource-limited settings, but with limitations, such as need for expert microscopists. An automated digital microscope artificial intelligence (Schistoscope), offers potential solution. This field study aimed to validate performance of Schistoscope detecting and quantifying Schistosoma haematobium eggs urine compared conventional...

Introduction: One of the global strategies for elimination schistosomiasis is by mass treatment school-aged children with a single oral dose praziquantel (40mg/kg) without prior individual diagnosis, target >75% coverage. This study was conducted to determine endemicity among and adults in Abuja, Nigeria. Methods: A total 1,370 participants were recruited which consisted 667 (48.67%) males 703 (51.31%) females. Urine stool specimens collected from each participant analyzed using...

Background: Granular assessment of Schistosomiasis (SCH) and soil-transmitted helminthiasis (STHs) in a population with instituted treatment programs is critical step understanding the progress control towards 2030 elimination targets. Such scientific insight into impact recurrent rounds mass Praziquantel Albendazole/Mebendazole should facilitate improved planning targeting resources. Methodology: This schistosomiasis STHs granular was conducted 16 Local Government Areas (LGAs) Ekiti State...

Lymphatic filariasis (LF) is a Neglected Tropical Disease (NTD) with high morbidity. Tools for detecting LF are either not readily available or used by Community Health Extension Workers (CHEWs) at Primary Centers. A newly developed diagnostic device, the AiDx Assist, targeted use CHEWs. The study aims to determine efficiency (speed) and effectiveness (diagnostic capacity) of CHEWs compared laboratory scientists new using World Organization's Target Product Profile (TPP) diagnostics as...

Schistosomiasis is a public health concern and there need for reliable field-compatible diagnostic methods in endemic settings. The AiDx Assist, an artificial intelligence (AI)-based automated microscope, has shown promising results the detection of Schistosoma haematobium eggs urine. It been further developed to detect mansoni stool. In this study, we evaluated performance Assist S. stool samples validated urine samples. Additionally, potential other helminths was explored. total, 405...

Introduction: One of the global strategies for elimination schistosomiasis is by Mass Drug Administration (MDA) a single oral dose praziquantel (40 mg/kg) without prior individual diagnosis, with target >75% treatment coverage among school-aged children. This study was conducted to determine endemicity children and adults in Abuja, Nigeria. Methods: A total 1370 participants were recruited, which consisted 667 (48.67%) males 703 (51.31%) females. Urine stool specimens collected from each...

Conventional microscopy is the standard procedure for diagnosis of schistosomiasis, despite its limited sensitivity, reliance on skilled personnel, and fact that it error prone. Here, we report performance innovative (semi-)automated Schistoscope 5.0 optical digital detection quantification Schistosoma haematobium eggs in urine, using conventional as reference standard. At baseline, 487 participants a rural setting Nigeria were assessed, which 166 (34.1%) tested S. positive by microscopy....

In this research, we report on the performance of automated optical digital detection and quantification Schistosoma haematobium provided by AiDx NTDx multi-diagnostic Assist microscope. Our study was community-based, a convenient sampling method used in 17 communities Abuja Nigeria, based disease prevalence information extracted from baseline database schistosomiasis, NTD Division, Federal Ministry Health. At baseline, samples 869 participants were evaluated which 358 (34.1%) tested S....

Designing new and inclusive diagnostic tools to detect Neglected Tropical Diseases (NTDs) achieve rational disease control requires a co-design process where end-users’ input is important. Failure involve all potential end-users in diagnostics for NTDs can result low use adoption failure, leading persistent infection hot spots ineffective control. There are different categories of NTD control, it unclear if there differences between the user efficiency, effectiveness, perception,...

Schistosomiasis is a treatable and preventable neglected tropical disease of Public Health importance affecting over 250 million people worldwide while Nigeria one the high burden countries. Currently available diagnoses are cumbersome, low in sensitivity not field-adaptable given skill required that rural settings where diseases majorly prevalent. Democratizing access to diagnosis with rapid, easy-to-use, accurate critical currently stepped-up control, pre-elimination elimination strategies...

We present a simple method for the diagnosis of urinary schistosomiasis using an in-line lensless holographic microscope combined with flow cytometry technique. Using image processing algorithms and binary classifier, our system provides automated detection Schistosoma haematobium eggs in infected urine samples. Registered hologram is reconstructed by applying backpropagation from sensor to sample plane automatically analysed presence S. eggs. Designed use resource-poor laboratory setting,...

We present an integrated optical wavelength meter based on a Si₃N₄/SiO₂ micro ring resonator (operating over free spectral range of ≈ 2.6 nm) whose output response is immune to temperature changes. The readout performed by neural network and non-linear optimization algorithm. This novel approach ensures high estimation precision (≈ 50 pm). observe long-term reproducibility the time interval one week. investigate influence ambient estimated...

Schistosomiasis is a treatable and preventable neglected tropical disease of Public Health importance affecting over 200 million people worldwide while Nigeria one the high burden countries. Currently, available diagnostic tests are cumbersome, low in sensitivity not field-adaptable given skills required that rural settings where diseases majorly prevalent. There an urgent need for easy to use automated device replace current gold standard, human-operated microscope. Many promising...

Schistosomiasis is a neglected tropical disease of Public Health importance affecting over 252 million people worldwide with Nigeria having very high number cases. It caused by blood flukes the genus Schistosoma and transmitted freshwater snails. To achieve current global elimination objectives, low-cost easy-to-use diagnostic tools are critically needed. Recent innovations in optical computer technologies have made handheld digital smartphone-based microscopes viable approach. Development,...

Community mobilisation is a vital process for raising awareness and increasing participation in healthcare interventions, research programs that require human sample collection mass management. In this report, we present the community approach undertaken implementation of granular schistosomiasis soil-transmitted helminths operational mapping assessment Ekiti State Nigeria. The was conducted 177 communities/wards 16 Local Government Areas. A total 15,340 urine stool samples were collected 34...

Community mobilisation is a vital process for raising awareness and increasing participation in healthcare interventions, research, programmes that require human sample collection mass management. In this report, we present the community approach undertaken implementation of operational mapping assessment granular schistosomiasis soil-transmitted helminths Ekiti State, Nigeria. The was conducted 177 communities/wards 16 local government areas. A total 15,340 urine stool samples were...

Community awareness and participation in mass screening is critical for schistosomiasis control. This study assessed the impact of sharing anonymized image-based positive test results on uptake during community mobilization outreach. We conducted an observational to compare population response standard strategies 14 communities Abuja, Nigeria. Six hundred ninety-one (341 females, 350 males) individuals participated this study. analyzed ratio, relative increase, sample collection time. The...

Fourier Ptychography is a computational imaging technique able to decouple high resolution from wide field of view, bypassing the diffraction limit microscope. Since it does not rely on precision mechanics or fluorescent imaging, practical interest for implementation in low scale devices. Despite its gains, realizing functional low-cost setup working at theoretical limits challenging due many factors causing discrepancies between theory and practice. Misalignment light emitting diode array...

We have implemented an extended depth of field optical system by wavefront coding with a micromachined membrane deformable mirror. This approach provides versatile extension to standard based on fixed phase mask. First experimental results validate the feasibility use adaptive optics for variable in imaging systems.