A5042043179- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Sperm and Testicular Function
- Hypothalamic control of reproductive hormones
- Enzyme Structure and Function
- Reproductive System and Pregnancy
- Genomics and Phylogenetic Studies
- Trauma and Emergency Care Studies
- Protein Structure and Dynamics
- Peptidase Inhibition and Analysis
- Molecular Biology Techniques and Applications
- Abdominal Trauma and Injuries
- Herpesvirus Infections and Treatments
- Cancer-related gene regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Ovarian function and disorders
- Natural Language Processing Techniques
- Forensic Toxicology and Drug Analysis
- Surgical Sutures and Adhesives
- Prenatal Screening and Diagnostics
- Animal Behavior and Reproduction
All India Institute of Medical Sciences
2023-2025
All India Institute of Medical Sciences Raipur
2024
All India Institute of Medical Sciences Bhopal
2024
Epicentre (South Africa)
2011
Indian Institute of Science Bangalore
1994-2009
University of North Carolina at Chapel Hill
2004
University of California, Davis
2004
Massachusetts Institute of Technology
1997-2002
Karlsruhe University of Education
1996
Software602 (Czechia)
1996
Various forms of birth control have been developed for women; however, there are currently few options men. The development male contraceptives that effective, safe, and reversible is desired family planning throughout the world. We now report contraception nonhuman primates (Macaca radiata) immunized with Eppin, a testis/epididymis-specific protein. Seven out nine males (78%) high titers to all these high-titer monkeys were infertile. Five seven (71%) high-anti-Eppin titer recovered...
RNA-seq has been widely adopted as a gene-expression measurement tool due to the detail, resolution, and sensitivity of transcript characterization that technique provides. Here we present two transposon-based methods efficiently construct high-quality libraries. We first describe method creates libraries for Illumina sequencing from double-stranded cDNA with only enzymatic reactions. generated little 10 pg mRNA (∼1 ng total RNA) this approach. also strand-specific library construction...
The difference spectrum of the quercetin—DNA complex versus quercetin alone was characterized by a peak at 395 nm. An increase in magnitude seen with increased ionic strength. Spectrophotometric changes absorbance and fluorescence showed that ethidium bromide is able to displace from — DNA complex. These results indicate binding does not involve electrostatic interactions but may be intercalative nature. Experiments using DNase I footprinting technique flavonoid possess any preferred sites...
The structure at the translation initiation region (TIR) of mRNA has pronounced regulatory effects on gene expression. Our attempts to overexpress C bacteriophage Mu in a variety expression vectors resulted low yields protein. Analysis shows potential form secondary involving ribosome binding site and AUG codon. We haveengineered overproduction protein using PCR-aided cloning approach remove sequences involved formation this structure. overexpressing clone, underthe control T7 10 promoter...
Non-operative management (NOM) has been validated for blunt liver and splenic injuries. Literature on continuous intra-abdominal pressure (IAP) monitoring as a part of NOM remains to be equivocal. The study aimed find any correlation between clinical parameters IAP, their effect the patients with injury. A prospective cross-sectional conducted at level I trauma center from October 2018 January 2020 including 174 who underwent following Hemodynamically unstable or those ventilators were...
The specific formylation of initiator methionyl-tRNA by formyltransferase (MTF; EC 2.1.2.9 ) is important for the initiation protein synthesis in eubacteria and eukaryotic organelles. determinants tRNA are clustered mostly acceptor stem. As part studies on molecular mechanism recognition MTF, we report here isolation characterization suppressor mutations Escherichia coli which compensate defect a mutant tRNA, lacking critical determinant We show that MTF has glycine-41 to arginine change...
The mode of recognition tRNAs by aminoacyl-tRNA synthetases and translation factors is largely unknown in archaebacteria. To study this process, we have cloned the wild type initiator tRNA gene from moderate halophilic archaebacterium<i>Haloferax volcanii</i> mutants derived it into a plasmid capable expressing these cells. Analysis <i>in vivo</i> show that aminoacylated but not formylated <i>H. volcanii</i>. This result provides direct support for notion protein synthesis archaebacteria...
Summary. Administration of Tamoxifen via the Alzet pump at a rate 50 μg hr- 1 for 90 days in adult male bonnet monkeys Macaca radiata had no effect on serum testosterone concentration determined 10 am and pm as well total sperm count 15-day intervals over period 260 days. However, significant reduction motility was observed beyond up until 225th day. Breeding studies conducted from day to revealed that these males were infertile.
Summary In the present study, our experience in collection of semen from adult bonnet monkeys and some characteristics are reported. Semen samples were collected by penile stimulation every 15 days over a period 3 years. The method ejaculation was more consistent with an increased volume semen. A distinct sesonality sperm count monkey observed. Sperm counts high during months August to October started declining November onwards. significantly (P<0.05) higher mid post-monsoon (July November)...
Formylation of initiator methionyl-tRNA by formyltransferase (MTF) is important for initiation protein synthesis in eubacteria. The determinants formylation are clustered mostly the acceptor stem tRNA. Previous studies suggested that a 16 amino acid insertion loop, present all eubacterial MTF's (residues 34-49 E. coli enzyme), plays an role specific recognition Here, we have analyzed effect site-specific mutations acids within this region. We show invariant arginine at position 42 loop very...
The contributions from the secondary structure of transcriptional activator protein C bacteriophage Mu to its specific DNA binding and influence various factors, viz., electrolytes, minor groove major binders on this protein−DNA interaction have been addressed. Circular dichroism (CD) spectral results suggest that, in absence Mg2+, exhibits a β-pleated sheetlike Mg2+ changes conformation more α-helical which could provide geometrical constraints complementary those helix. Thus, acts as...
Hormonal approaches to male contraception that are based on the suppression of LH secretion require androgen replacement treatment maintain sexual behaviour and secondary characteristics. Androgen supplementation not only involves large frequent doses testosterone esters but also results in undesirable effects prostate gland. In an attempt avoid such problems, a synthetic androgen, 7alpha-methyl-19-nortestosterone (MENT), which is much more potent than testosterone, has been developed....
A 16-aa insertion loop present in eubacterial methionyl-tRNA formyltransferases (MTF) is critical for specific recognition of the initiator tRNA Escherichia coli . We have studied interactions between this region E. enzyme and (Met-tRNA) by using two complementary protection experiments: MTF against proteolytic cleavage nucleolytic MTF. The uniquely sensitive to trypsin. show that substrate Met-tRNA protects trypsin cleavage, whereas a formylation-defective mutant Met-tRNA, which binds with...
The formylation of initiator methionyl-tRNA by formyltransferase (MTF) is important for the initiation protein synthesis in eubacteria. We are studying molecular mechanisms recognition tRNA Escherichia coli MTF. MTF from eubacteria contains an approximately 100-amino acid C-terminal extension that not found E. glycinamide ribonucleotide formyltransferase, which, like MTF, use N10-formyltetrahydrofolate as a formyl group donor. This extension, which forms distinct structural domain, attached...
Protein synthesis in eukaryotic cytoplasm and archaebacteria is initiated with methionine, whereas, that eubacteria organelles, such as mitochondria chloroplasts, formylmethionine. In view of this clear distinction, we have investigated whether protein the can be formylmethionine, and, if so, what consequences are to cell. For purpose, expressed an inducible manner Escherichia coli methionyl-tRNA formyltransferase (MTF) yeast Saccharomyces cerevisiae. Expression active MTF, but not inactive...