A5013750754- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cell Image Analysis Techniques
- Calcium signaling and nucleotide metabolism
- Sirtuins and Resveratrol in Medicine
- Single-cell and spatial transcriptomics
- Renal Transplantation Outcomes and Treatments
- Heart Failure Treatment and Management
- Urologic and reproductive health conditions
- 3D Printing in Biomedical Research
- Genital Health and Disease
- Microbial Inactivation Methods
- Acute Lymphoblastic Leukemia research
- Peptidase Inhibition and Analysis
- Advanced Fluorescence Microscopy Techniques
- Renal Diseases and Glomerulopathies
- Cervical Cancer and HPV Research
- Industrial Vision Systems and Defect Detection
- Transgenic Plants and Applications
- Proteoglycans and glycosaminoglycans research
- Signaling Pathways in Disease
- Adolescent and Pediatric Healthcare
- Glycosylation and Glycoproteins Research
Medical University of South Carolina
2018-2021
University of South Carolina
2018
Fordham University
2013-2017
Istanbul University
2008-2010
PharmacoGenetics (China)
2009
Mayo Clinic in Arizona
2008
Mayo Clinic
2006
Başkent University
2001-2004
Obesity is one of the major health problems our times. Elucidating signaling mechanisms by which high-fat caloric diet induces obesity critical for understanding this condition and development therapeutic strategies its treatment. Here, we demonstrate a novel role protein CD38 as regulator body weight during diet. ubiquitous enzyme that catalyzes synthesis second messengers has been implicated in regulation wide variety pathways. We report CD38-deficient mice are protected against...
Multiplexed in-situ fluorescent imaging offers several advantages over single-cell assays that do not preserve the spatial characteristics of biological samples. This information, in addition to morphological properties and extensive intracellular or surface marker profiling, comprise promising avenues for rapid advancements understanding disease progression diagnosis. As protocols conducting such experiments continue improve, it is intent this study provide validate software processing...
Background 5′-Nucleotidases play a critical role in nucleotide pool balance and the metabolism of nucleoside analogs such as gemcitabine cytosine arabinoside (AraC). We previously performed an expression array association study with AraC cytotoxicity using 197 human lymphoblastoid cell lines. One gene that was significantly associated nucleotidase family member, NT5C3. Very little is known regard to pharmacogenomics this enzymes. Methods set out identify common genetic variation NT5C3 by...
Gemcitabine and other cytidine antimetabolites require metabolic activation by phosphorylation. Deoxycytidine kinase (DCK) monophosphate (CMPK) catalyze these reactions. We have applied a genotype-to-phenotype strategy to study DCK CMPK pharmacogenomics. Specifically, we resequenced <i>DCK</i> <i>CMPK</i> using 240 DNA samples, 60 each from African-American, Caucasian-American, Han Chinese-American, Mexican-American subjects. observed 28 polymorphisms in <i>CMPK</i>, 33 of which were novel....
Abstract Electroporation is the most feasible non-viral material delivery system for manipulating human T cells given its time- and cost-effectiveness. However, efficient requires electroporation settings to be optimized different devices, cellular states, materials delivered. Here, we used either induce exogenous gene expression in primary by plasmids or vitro transcribed (IVT) mRNA also target endogenous genes Cas9 ribonucleoproteins (RNPs). We characterized conditions both activated...
Persistent infection with high-risk human papillomavirus (HPV) genotype is a major factor leading to many cancers. Mechanisms of HPV entry into host cells and genome trafficking towards the nucleus are incompletely understood. Dopachrome tautomerase (DCT) was identified as cellular gene required for in HeLa on siRNA screen study. Here, we confirm that DCT knockdown significantly decreases keratinocyte HaCaT observed HeLas. We investigated effects found depletion caused increased reactive...
Research on renal biopsies has shown that HLA class I antigens are distributed throughout the parenchyma, but distribution of HLA-DR varies greatly. We investigated expression in 90 transplants, and also semiquantitatively assessed proportions CD68-, CD3-, HLA-DR-positive infiltrating cells by immunohistochemistry. The relationships between tubular DR interstitial lymphocyte macrophage infiltration were examined. Forty showed acute rejection (AR), 33 chronic (CR), 10 suspected (SR), 7 no...
Human primary T cells are invaluable and feasible model systems to study the characteristics of immune in various contexts, including but not limited cancer immunotherapy. Following isolation from fresh human blood samples, it is possible culture, expand, manipulate these cells, which allows extensive investigation for research purposes. Techniques handling well-established parts protocols can highly vary across different labs. These differences there due historical reasons often only...
Abstract Background Multiplexed in-situ fluorescent imaging offers several advantages over single-cell assays that do not preserve the spatial characteristics of biological samples. This information, in addition to morphological properties and extensive intracellular or surface marker profiling, comprise promising avenues for rapid advancements understanding disease progression diagnosis. As protocols conducting such experiments continue improve, it is intent this study provide validate...
No AccessJournal of UrologyCLINICAL UROLOGY: Case Reports1 Sep 2001PLASMA CELL GRANULOMA OF THE TESTIS: UNUSUAL LOCALIZATION PINAR KAYNAK AKSOY, B. HANDAN ÖZDEMIR, CEM AYGÜN, and MUHTESEM AGILDERE AKSOYPINAR AKSOY More articles by this author , ÖZDEMIRB. ÖZDEMIR AYGÜNCEM AYGÜN AGILDEREMUHTESEM View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)65893-4AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail...
Human primary T cells are invaluable and feasible model systems to study the characteristics of immune in various contexts, including but not limited cancer immunotherapy. Following isolation from fresh human blood samples, it is possible culture, expand, manipulate these cells, which allows extensive investigation for research purposes. Techniques handling well-established parts protocols can highly vary across different labs. These differences there due historical reasons often only...
Systemic Inflammatory Response Syndrome (SIRS) is a pathophysiologic state associated with trauma and major surgery. cytokines plays an important role in the pathogenesis of SIRS. Cytokines induce activation classic hypothalamic-pituitary stress response that leads to increase secretion hormone ACTH hyperglycemia. HMG-CoA reductase inhibitors, such as statins, have been shown anti-inflammatory properties. Recent studies humans indicate perioperative use statins may decrease morbidity...
This protocol describes an imaged-based 3-dimensional (3D) cell culture cytotoxicity assay using multicellular tumor spheroids (in this case MC38 cells) sensitized with hgp100 peptide and killing them pmel-1 T cells or the apoptosis-inducing antibiotic, Staurosporine. Our goal was to explore in vitro 3D system study cell-mediated as a potential way more rapidly relevantly test effect of manipulation on before moving mouse model. In setup, are required migrate towards that already suspended...
Abstract We evaluated the utility of single-cell sequencing tumor-infiltrating lymphocytes (TIL) for tumor-reactive T-cell receptor (TCR) discovery. Using MC38 cell line as our tumor model in mice, we show that expression exogenous TCRs via mRNA electroporation human T cells provides an easy and quick path to validating tumor-specific candidate TCRs. detail identification validation four novel MC38-reactive mouse with varying levels reactivity target cells. Validating process, one is...
Natriuretic peptides (NP) undergo enzymatic hydrolysis catalyzed by membrane metallo-endopeptidase (MME). Genetic variation in MME has not been addressed resequencing the gene different ethnic populations. DNA samples from 96 Caucasian American, African and Han Chinese American subjects, part of “Human Variation Panel” were obtained Coriell Cell Repository resequenced. Ninety polymorphisms identified 3 groups, including 8 nonsynonymous single nucleotide (SNP) that resulted following changes...