A5101747454- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Photoreceptor and optogenetics research
- Genomics and Chromatin Dynamics
- Photochromic and Fluorescence Chemistry
- Cell Image Analysis Techniques
- Retinal Diseases and Treatments
- CRISPR and Genetic Engineering
- Renal and related cancers
- Neuroscience and Neural Engineering
- Stellar, planetary, and galactic studies
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Genetic and Kidney Cyst Diseases
- Retinopathy of Prematurity Studies
- Astrophysics and Cosmic Phenomena
- Galaxies: Formation, Evolution, Phenomena
- Gene Regulatory Network Analysis
University of Illinois Urbana-Champaign
2024
National Eye Institute
2020-2023
National Institutes of Health
2020-2023
Government of the United States of America
2023
Shanghai Jiao Tong University
2010-2017
Shanghai Institutes for Biological Sciences
2010
Mutations in the photoreceptor transcription factor gene cone-rod homeobox (CRX) lead to distinct retinopathy phenotypes, including early-onset vision impairment dominant Leber congenital amaurosis (LCA). Using induced pluripotent stem cells (iPSCs) from a patient with CRX-I138fs48 mutation, we established an vitro model of CRX-LCA retinal organoids that showed defective maturation by histology and profiling, diminished expression visual opsins. Adeno-associated virus (AAV)-mediated CRX...
Mutations in the IQ calmodulin-binding motif containing B1 (IQCB1)/NPHP5 gene encoding ciliary protein nephrocystin 5 cause early-onset blinding disease Leber congenital amaurosis (LCA), together with kidney dysfunction Senior-Løken syndrome. For vitro modeling, we obtained dermal fibroblasts from patients NPHP5-LCA that were reprogrammed into induced pluripotent stem cells (iPSCs) and differentiated retinal pigment epithelium (RPE) organoids. Patient RPE demonstrated aberrantly elongated...
Abstract Introduction Degenerative retinal diseases like age-related macular degeneration (AMD) are the leading cause of blindness. Cell transplantation showed promising therapeutic effect for such diseases, and embryonic stem cell (ESC) is one sources donor cells. Here, we aimed to generate progenitor cells (RPCs) from rat ESCs (rESCs) test their effects in model. Methods The rESCs (DA8-16) were cultured N2B27 medium with 2i, differentiated two types RPCs following SFEBq method...
Abstract We present a flare star catalog from 4 yr of nontargeted millimeter-wave survey data the South Pole Telescope (SPT). The were taken with SPT-3G camera and cover 1500 deg 2 region sky 20 h 40 m 0 s to 3 in right ascension −42° −70° declination. This was observed on nearly daily cadence 2019 2022 chosen avoid plane galaxy. A short-duration transient search this yields 111 flaring events 66 stars, increasing number both detected stars by an order magnitude previous release. provide...
We have generated a high-resolution Hi-C map of developing human retinal organoids to elucidate spatiotemporal dynamics genomic architecture and its relationship with gene expression patterns. demonstrate progressive stage-specific alterations in DNA topology correlate these changes transcription cell-type-restricted markers during differentiation. Temporal reveals shift toward A compartment for protein-coding genes B non-coding RNAs, displaying high low expression, respectively. Notably,...
We have generated high-resolution Hi-C map of developing human retinal organoids to elucidate spatiotemporal dynamics genomic architecture and its relationship with gene expression patterns. demonstrate progressive developmental stage-specific alterations in DNA topology that correlates transcription cell-type restricted genes during differentiation. Temporal reveals a shift towards A compartment for protein-coding B non-coding RNAs, displaying high low respectively. Notably, retina-enriched...