A5031038848- Pancreatic function and diabetes
- Pancreatic and Hepatic Oncology Research
- Pluripotent Stem Cells Research
- Pancreatitis Pathology and Treatment
- Renal and related cancers
- Liquid Crystal Research Advancements
- Congenital heart defects research
- Surfactants and Colloidal Systems
- High-pressure geophysics and materials
- Genetics and Neurodevelopmental Disorders
- Cholinesterase and Neurodegenerative Diseases
- Energetic Materials and Combustion
- Glycosylation and Glycoproteins Research
- Phagocytosis and Immune Regulation
- NF-κB Signaling Pathways
- Metabolism and Genetic Disorders
- Catalysts for Methane Reforming
- Skin Protection and Aging
- Neuroendocrine Tumor Research Advances
- Coal Combustion and Slurry Processing
- Air Quality Monitoring and Forecasting
- Atmospheric Ozone and Climate
- Advancements in Transdermal Drug Delivery
- Minerals Flotation and Separation Techniques
- Cell Adhesion Molecules Research
The University of Texas Southwestern Medical Center
2002-2024
Southwestern Medical Center
2015-2024
Howard Hughes Medical Institute
2002
Nagoya University
2002
Walter and Eliza Hall Institute of Medical Research
2002
The University of Texas at Austin
1998-2001
North Carolina State University
1995
Texas A&M University
1989
The University of Texas System
1988
Auburn University
1985
To investigate the role of HOX-like homeoprotein PDX1 in formation and maintenance pancreas, we have genetically engineered mice so that only source is a transgene can be controlled by application tetracycline or its analogue doxycycline. In these coding region for tetracycline-regulated transactivator (tTA off ) has replaced endogenous Pdx1 gene to ensure correct temporal spatial expression regulatable transactivator. absence doxycycline, tTA activates transcription bicistronic encoding an...
Pancreatic agenesis is a human disorder caused by defects in pancreas development. To date, only few genes have been linked to pancreatic humans, with mutations and duodenal homeobox 1 (PDX1) pancreas-specific transcription factor 1a (PTF1A) reported 5 families described cases. Recently, GATA6 identified large percentage of cases, GATA4 mutant allele has implicated single case. In the mouse, Gata4 Gata6 are expressed several endoderm-derived tissues, including pancreas. analyze functions...
The orphan nuclear receptor NR5A2 is necessary for the stem-like properties of epiblast pre-gastrulation embryo and cellular physiological homeostasis endoderm-derived organs postnatally. Using conditional gene inactivation, we show that Nr5a2 also plays crucial regulatory roles during organogenesis. During formation pancreas, expansion nascent pancreatic epithelium, subsequent multipotent progenitor cell (MPC) population gives rise to pre-acinar cells bipotent with ductal islet endocrine...
Maintenance of cell type identity is crucial for health, yet little known the regulation that sustains long-term stability differentiated phenotypes. To investigate roles key transcriptional regulators play in adult cells, we examined effects depletion developmental master regulator PTF1A on specialized phenotype pancreatic acinar vivo. Transcriptome sequencing and chromatin immunoprecipitation results showed maintains expression genes all cellular processes dedicated to production secretory...
AbstractThe basic helix-loop-helix (bHLH) transcription factor PTF1a is critical to the development of embryonic pancreas. It required early for formation undifferentiated tubular epithelium nascent pancreatic rudiment and then becomes restricted differentiating acinar cells, where it directs transcriptional activation secretory digestive enzyme genes. Here we report that complex temporal spatial expression Ptf1a controlled by at least three separable gene-flanking regions. A 14.8-kb control...
The 3' region of the Anabaena variabilis nifD gene contains an 11-kilobase-pair element which is excised from chromosome during heterocyst differentiation. We have sequenced recombination sites border in vegetative cells and rearranged sequences. In cells, was flanked by 11-base-pair direct repeats were identical to present at ends sp. strain PCC 7120 (Anabaena 7120). Although A. are quite distinct many ways, overall sequence similarity between two strains for regions 96%. Like element,...
The timing and gene regulatory logic of organ-fate commitment from within the posterior foregut mammalian endoderm is largely unexplored. Transient misexpression a presumed pancreatic-commitment transcription factor, Ptf1a, in embryonic mouse (Ptf1aEDD) dramatically expanded pancreatic network foregut. Ptf1aEDD temporarily suppressed Sox2 broadly over anterior endoderm. Pancreas-proximal organ territories underwent full tissue conversion. Early-stage rapidly endogenous endodermal...
A cDNA encoding the mature E1 beta subunit of bovine branched-chain alpha-keto acid dehydrogenase complex was isolated from a lambda ZAP expression library. The is 1,393 base pairs in length. It encodes entire consisting 342 amino residues and partial mitochondrial targeting presequence 26 residues. calculated molecular mass 37,776 daltons, isoelectric point pI 5.04. expressed Escherichia coli via pKK233-2 vector presence isopropyl beta-D-thiogalactopyranoside (IPTG). When induced by IPTG at...
Pancreatic ductal adenocarcinoma (PDA) is the fourth leading cause of cancer-related deaths in United States, and projected to be second by 2025. It has worst survival rate among all major cancers. Two pressing needs for extending life expectancy affected individuals are development new approaches identify improved therapeutics, addressed herein, identification early markers. PDA advances through a complex series intercellular physiological interactions that drive cancer progression response...
Topical treatment of female SENCAR mice with 12- O -tetradecanoylphorbol- 13-acetate (TPA) reduced both dermal and epidermal catalase-specific activities 38% 51% within 6 h 18 promoter application, respectively. Dermal catalase activity recovered to control levels 72 whereas remained suppressed. Activity measurements were also made in four subpopulations keratinocytes prepared by Percoll gradient centrifugation that differed their stages differentiation. Catalase-specific increased...
Abstract A procedure has been developed which permits the direct analysis of powdered whole-coal samples by atomic absorption or emission methods using conventional instrumentation, thereby eliminating time-consuming ashing and sample-digestion procedures. Linear plots signal intensity versus elemental concentration analyzed were obtained for several elements. Preliminary results indicate this method is applicable estimation both minor-level (eg., Fe Ca) trace-level Pb Zn) constituents.
Chiral, smectogenic pendant groups have been covalently attached to cyclic oligosiloxanes by hydrosilylation. These materials and the olefinic compounds from which they were derived exhibit ferroelectric behavior. The mesomorphic range, in particular S C * phase, of these oligomers is greatly enhanced relative vinylogous parents. electro-optic thermal properties, addition synthetic procedures used prepare materials, are described detail. seen as potential precursors liquid crystalline...
Unusual electric field induced phase transitions have been demonstrated in laterally linked smectic C liquid crystals that possess two chiral carbons. The materials a rich polymorphism including an apparent antiferroelectric which occurs at temperatures immediately below the A to transition. Another tristable has observed closer crystal temperature-dependent electrooptic response and spontaneous polarization measurements for title compounds are compared with those of monomeric similar...
Diagnostic markers are desperately needed for the early detection of pancreatic ductal adenocarcinoma (PDA). We describe sets expressed in temporal order mouse models during pancreatitis, PDA initiation and progression. Cell type specificity differential expression were identified by screening single cell (sc) RNAseq from tumor samples a model (KIC) at late stages progression compared to that normal pancreas. Candidate genes three sources: (1) an unsupervised preferentially tumors; (2)...
Proper maintenance of mature cellular phenotypes is essential for stable physiology, suppression disease states, and resistance to oncogenic transformation. We describe the transcriptional regulatory roles four key DNA-binding transcription factors (Ptf1a, Nr5a2, Foxa2 Gata4) that sit at top a hierarchy controlling all aspects highly differentiated cell-type–the pancreatic acinar cell (PAC). Selective inactivation Ptf1a , Nr5a2 Gata4 individually in mouse adult PACs rapidly altered...
We describe here an apparatus for generating ultrahigh pressures and temperatures materials synthesis. The system uses a spherically shaped explosive charge to radially compress various contained within the sample cavity. results show is completely reproducible and, because of geometry loading system, workpiece that holds precursor material in excellent postshock condition. Samples are subjected pressure pulse approximately 230 GPa period 4 μs. Using this C60 has been compressed using...
The mechanism by which a tristable, laterally linked, SC* liquid crystal dimer reorients under the application of an electric field has been investigated combination X-ray diffraction studies and torsional viscosity measurements. A new experimental cell developed that allows direct measurement layer spacing as function applied at controlled temperature. distance between smectic layers is consistent with naturally arranging in "U" conformation. No significant change was observed upon for all...
Abstract An explosive-based spherical shock-recovery system was used to load an annealed solid steel sphere. The hydrocode CTH utilized model the explosivemetal interaction. Based on simulation results, pressures reached as high l00 GPa (1 Mbar) at surface and converged 1.25 TPa (12.5 center of As computer predicted, post-shock examination recovered sphere revealed a large void its created result strong tensile waves emanating from surface. Micro-hardness measurements showed hardness...