A5103154997- biodegradable polymer synthesis and properties
- Pulmonary Hypertension Research and Treatments
- Bone Tissue Engineering Materials
- Nitric Oxide and Endothelin Effects
- Fungal and yeast genetics research
- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Liquid Crystal Research Advancements
- Glycosylation and Glycoproteins Research
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Asthma and respiratory diseases
- Toxin Mechanisms and Immunotoxins
- Electrospun Nanofibers in Biomedical Applications
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Advanced Drug Delivery Systems
- Molecular spectroscopy and chirality
- Computational Drug Discovery Methods
- Metabolism, Diabetes, and Cancer
- Fibroblast Growth Factor Research
- Complement system in diseases
- Ocular Disorders and Treatments
University of Michigan
2021-2024
BioSurfaces (United States)
2021-2024
Twist Bioscience (United States)
2023
University of Pittsburgh
2004-2022
University of Southern Maine
2014
Bowling Green State University
2011
Tetracore (United States)
2008-2011
Washington State University
2004
Boston University
2002-2003
University of Oxford
1997
Glucose-6-phosphate dehydrogenase (G6PD), the first enzyme of pentose phosphate pathway, is principal intracellular source NADPH. NADPH utilized as a cofactor by vascular endothelial cell nitric-oxide synthase (eNOS) to generate nitric oxide (NO•). To determine whether G6PD modulates NO•-mediated angiogenesis, we decreased expression in bovine aortic cells using an antisense oligodeoxynucleotide or increased adenoviral gene transfer, and examined growth factor (VEGF)-stimulated...
Computational techniques such as structure-based virtual screening require carefully prepared 3D models of potential small-molecule ligands. Though powerful, existing commercial programs for virtual-library preparation have restrictive and/or expensive licenses. Freely available alternatives, though often effective, do not fully account all possible ionization, tautomeric, and ring-conformational variants. We here present Gypsum-DL, a free, robust open-source program that addresses these...
Abstract The transcription of genes located in subtelomeric regions yeast chromosomes is repressed relative to the rest genome. This repression requires wild-type nucleosome levels but not telomere silencing factors Sir2, Sir3, Sir4, and Rap1. Subtelomeric heterochromatin characterized by absence acetylation or methylation histone H3 lysine residues, it known whether hypoacetylation hypomethylation a prerequisite for establishment heterochromatin. We have systematically mutated N-terminal...
The rate-limiting step in protein secretion is folding, which occurs the endoplasmic reticulum (ER) lumen, and almost all secreted proteins contain disulfide bonds that form ER stabilize native state. Secreted unable to fold may aggregate or they be subject ER-associated degradation. To examine fate of aberrant forms a well characterized, disulfide-bonded protein, we expressed bovine pancreatic trypsin inhibitor yeast. Bovine single domain, 58-amino acid polypeptide containing three bonds,...
Increased expression of 5-lipoxygenase (5LO) in pulmonary artery endothelial cells (PAECs) has been observed disease states such as hypertension and allergen challenge. To understand the function 5LO, we examined this enzyme normally cultured human PAECs its characteristics when overexpressed. A small amount 5LO message protein was detected by reverse-transcriptase-mediated PCR (RT—PCR) Western blotting PAECs. Sequencing RT—PCR products that overlapped entire coding region mRNA indicated...
Increased 5-lipoxygenase (5LO) expression in pulmonary artery endothelial cells (PAECs) has been observed primary hypertension, a disorder associated with vascular remodeling and aberrant cell proliferation. To examine whether 5LO plays role proliferation, we analyzed the effect of inhibitors on cultured human PAECs. Analysis [ 3 H]thymidine incorporation showed that 5LO-activating protein AA-861, nordihydroguaiaretic acid (NDGA), MK-886 all inhibited PAEC growth dose-dependent manner,...
Llama derived single domain antibodies (sdAb), the recombinantly expressed variable heavy domains from unique heavy-chain only of camelids, were isolated a library llamas immunized with commercial abrin toxoid preparation. Abrin is potent toxin similar to ricin in structure, sequence and mechanism action. The selected sdAb evaluated for their ability bind as well abrax (a recombinant A-chain), purified fractions, Abrus agglutinin protein related but lower toxicity), ricin, unrelated...
Molecules that mediate reproductive interactions are some of the most rapidly evolving traits. Researchers have often suggested this is due to coevolution at key physiological interfaces. However, very few these interfaces well understood functional level. One such interface digestion spermatophore in Lepidoptera. Female Lepidoptera a specialized organ called bursa copulatrix receives and processes male spermatophore, complex proteinaceous ejaculate. In cabbage white butterfly, Pieris rapae,...
Glucose is central to many biological processes, serving as an energy source and a building block for biosynthesis. After glucose enters the cell, hexokinases convert it glucose-6-phosphate (Glc-6P) use in anaerobic fermentation, aerobic oxidative phosphorylation, pentose-phosphate pathway. We here describe genetic screen Saccharomyces cerevisiae that generated novel spontaneous mutation hexokinase-2, hxk2G238V, confers resistance toxic analog 2-deoxyglucose (2DG). Wild-type 2DG...
Given that many antifungal medications are susceptible to evolved resistance, there is a need for novel drugs with unique mechanisms of action. Inhibiting the essential proton pump Pma1p, P-type ATPase, potentially effective therapeutic approach orthogonal existing treatments. We identify NSC11668 and hitachimycin as structurally distinct antifungals inhibit yeast ScPma1p. These compounds provide new opportunities drug discovery aimed at this important target.
Increased expression of 5-lipoxygenase (5LO) in pulmonary artery endothelial cells (PAECs) has been observed disease states such as hypertension and allergen challenge. To understand the function 5LO, we examined this enzyme normally cultured human PAECs its characteristics when overexpressed. A small amount 5LO message protein was detected by reverse-transcriptase-mediated PCR (RT—PCR) Western blotting PAECs. Sequencing RT—PCR products that overlapped entire coding region mRNA indicated...
ABSTRACT In vivo discovery approaches for single domain antibodies such as VHH have been limited by the lack of methodologies available camelid B cell interrogation. Here, we report a novel application Berkeley Lights Beacon® optofluidic platform to heavy-chain-only screening cells from alpacas immunized with two different targets. Custom methods alpaca enrichment, culture, on-Beacon IgG2/3 detection, and sequencing were developed used discover target-specific candidates an either human...
In vivo VHH discovery approaches have been limited by the lack of methodologies for camelid B cell interrogation. Here, we report a novel application Beacon® optofluidic platform to heavy-chain-only antibodies screening alpaca cells. Methods enrichment, culture, IgG2/3 detection, and sequencing were developed used discover target-specific from an immunized with prostate-specific membrane antigen (PSMA) or second target. PSMA-specific hits expressed as VHH-Fc characterized using label-free...
Abstract Pretransitional optical activity measurements in smectic liquid crystals of both law and high chirality are reported. In the low sample, p-(n-(decyloxybenzylidene)-p-amino-(2-methylbutyl)cin-namate (DOBAMBC), results indicate that isotropic phase just above A C* can be explained terms a Landau-deGennes free energy with no coupling between chiral modes. 1-methylheptyl 4′-[(4″-tetradecyloxyphenyl)propioloyloxy]biphenyl-4-carboxylate (14PlM7), data recently discovered A* cannot this...
Abstract MutantHuntWGS is a user-friendly pipeline for analyzing Saccharomyces cerevisiae whole-genome sequencing data. It uses available open-source programs to: (1) perform sequence alignments paired and single-end reads, (2) call variants, (3) predict variant effect severity. outputs shortlist of variants while also enabling access to all intermediate files. To demonstrate its utility, we use assess multiple published datasets; in cases, it detects the same causal reported literature....
Abstract Copolymers of lactic (or lactide) and glycolic glycolide) acids (PLGAs) are among the most commonly used materials in biomedical applications, especially parenteral controlled drug delivery due to their biocompatibility, predictable degradation rate, ease processing. Besides manufacturing variables vehicles, changes PLGA raw material properties can affect product behavior. Accordingly, an in-depth understanding polymer-related “critical quality attributes” improve selection...