The aim of this study was to explore the potential effect electroacupuncture (EA) at ST36 on mice bearing breast tumors by regulating inflammatory cytokines enhance antitumor immunity via vagus nerve.Female BALB/c were implanted with 4T1-luc2 tumor cells establish a murine mammary cancer model. Tumor growth evaluated volume, weight and bioluminescence imaging. Inflammatory conditions in serum tissue assessed (IL-1β, TNF-α IL-10) HE staining. Proportions functions CD8+ T cells, NK MDSCs...

Abstract The efficacy of acupuncture in treating pain diseases has been recognized clinical practice, and its mechanism action a hot topic academic research. Previous basic research on analgesia focused mostly the nervous system, with few studies addressing immune system as potential pathway analgesia. In this study, we investigated effect electroacupuncture (EA) β-endorphins (β-END) content, END-containing leukocyte type number, sympathetic neurotransmitter norepinephrine (NE), chemokine...

Ulcerative colitis (UC) is a type of inflammatory bowel disease (IBD) characterized by chronic inflammation colon. It commonly believed that the imbalance immune system and overwhelming production cytokines are involved in pathogenesis UC. Recent studies demonstrated interleukin-35 (IL-35), key player regulation inflammation, has been identified as potential therapeutic target to treat However, conventional intravenous administration costly inconvenient. The present study was designed...

Introduction Recent research has focused on the local control of articular inflammation through neuronal stimulation to avoid systemic side effects conventional pharmacological therapies. Electroacupuncture (EA) been proven be useful for suppressing and pain reduction in knee osteoarthritis (KOA) patients, yet its mechanism remains unclear. Methods In present study, KOA model was established using intra-articular injection sodium monoiodoacetate (MIA) (1 mg/50 μL) into cavity. EA delivered...

Although referred pain or hypersensitivity has been repeatedly reported in irritable bowel syndrome (IBS) patients and experimental colitis rodents, little is known about the neural mechanisms. Spinal long-term potentiation (LTP) of nociceptive synaptic transmission plays a critical role development somatic hyperalgesia chronic conditions. Herein, we sought to determine whether spinal LTP contributes referral rats particularly electroacupuncture (EA) effective alleviate via suppressing LTP....

Recent studies have shown that both superficial and deep acupuncture produced clinically relevant persistent effect on chronic pain, several subtypes of somatic primary afferents played critical roles in moxibustion analgesia. However, which kind the tissue acupoint is activated by or to relieve pain persistently remains unclear. The aim this study investigate distinct peripheral different layers (muscle skin) for relief. Muscular A-fibers electroacupuncture (dEA) with lower intensity...

Abstract Transcutaneous electrical acupoint stimulation (TEAS) has been consistently used clinically for its ease of operation, non-invasiveness and painlessness, in contrast to the characteristics inserted needles. However, mechanism remains unknown. The aim this study was investigate local response TEAS at Hegu (LI4). Immunohistochemistry measure expression tryptase-positive mast cells, neuropeptides calcitonin gene-related peptide (CGRP) substance P (SP) LI4. Mast cells were also labelled...

Acupuncture is an effective alternative therapy for pain management. Evidence suggests that acupuncture relieves by exciting somatic afferent nerve fibers. However, the mechanism underlying interaction between neurons in different layers of spinal dorsal horn induced electroacupuncture (EA) remains unclear. The aim this study was to explore EA relieving inflammatory muscle pain, which associated with activation spontaneous firing low-threshold mechanoreceptor (LTM) and inhibition wide...

Abstract Background Ultraviolet radiation causes skin photoaging by producing a variety of enzymes, which impact both health and hinder beauty. Currently, the early diagnosis treatment remain challenge. Bioinformatics analysis has strong advantages in exploring core genes biological pathways photoaging. Aims To screen validate key risk associated with plasminogen to identify potential target for Methods Two human transcriptome datasets were obtained searching Gene Expression Omnibus (GEO)...

OBJECTIVE To observe and compare the ameliorate effects of electroacupuncture (EA) transcutaneous electrical acupoint stimulation (TEAS) with different intensities on inflammatory muscle pain, so as to confirm role afferent nerve fibers in layers (skin or muscle) pain relief acupuncture. METHODS The thresholds A (Ta) C (Tc) were selected for TEAS EA. EA applied layer skin Liangqiu (ST34), respectively. Forty-eight rats randomly divided into control, model, TEAS-Ta, TEAS-Tc, EA-Ta EA-Tc...

To explore the effects of moxibustion intervention on wound healing in rats with full-thickness cutaneous wounds.A total 28 adult SD male were randomly assigned to model group and (n＝14 each one). The skin was established by removal a piece from median line rats' back (about 2 cm below shoulder blade). Moxibustion applied surrounding area focus for 25 min, once daily 6 days. observed photographed after intervention. tissues harvested transcardiac perfusion 4% paraformaldehyde solution 2nd...

Our previous study demonstrated that moxibustion could promote skin wound healing in rats with full-thickness cutaneous wounds. The present was designed to observe the effect of on levels pro-inflammatory and anti-inflammatory cytokines, proliferation vascular endothelial cells, so as explore its mechanisms underlying facilitating wound-healing.A total 84 adult SD male were randomly assigned normal (n＝6), model(n＝39)and moxibustion(n＝39)groups. model established by removal a piece from...

To investigate the mechanism of electroacupuncture (EA) "Zusanli" (ST36) in delaying colon "inflammation-cancer transformation" mice by anti-inflammatory.C57BL/6J were randomly divided into normal, model and EA groups, with 12 each group. The mouse colorectal cancer (CRC) was established intrape-ritoneal injection azomethane (AOM) feeding dextran sodium sulfate (DSS). At beginning 2nd cycle, applied to bilateral ST36 for 30 min once every other day times. number tumors group observed, weight...

To determine whether electroacupuncture (EA) or moxibustion-like stimulation (MLS) can affect the cutaneous and/or systemic hypothalamic-pituitary-adrenal (HPA) axes.Rats were divided into Control, EA, 37°C MLS and 43.5°C groups. EA performed at bilateral ST36 LI4. The expression of corticotropin-releasing factor (CRF), adrenocorticotropic hormone (ACTH) glucocorticoid receptor (GR) was detected in local tissues site LI4 by immunohistochemical staining. In addition, levels CRF, ACTH...

<title>Abstract</title> This article proposes a new process for fabricating polymer microneedles (MNs) patch integrated with an LED light source (LED-HEMA/MN, LH-MN) using ultraviolet (UV) curing technology. Hydroxyethyl methacrylate (HEMA) is used as the base material, focus on studying active fabrication of MNs patch. The study also investigates morphology, size, mechanical properties, ex vivo skin penetration performance, operating temperature, and performance optimization LH-MN....

The NIH-funded "Stimulating Peripheral Activity to Relieve Conditions (SPARC)" program has been initiated in Octomber 2016, aiming at developing high resolution neural circuit maps and next generation modulation devices. This brought great stimulus acupuncturists acupuncture researchers both home abroad. Is the SPARC a driving force or challenge of research? In present study, we introduced compared it with current research. first step is better map circuits associated disease on anatomical...

Skin innervation is an important part of the peripheral nervous system. Although study cutaneous nerve fibers has progressed rapidly, most understanding their distributional and chemical characteristics comes from conventional histochemical immunohistochemical staining on thin tissue sections. With development clearing technique, it become possible to view thicker The present protocol describes multiple fluorescent sections at a thickness 300 µm plantar dorsal skin rat hindfoot, two typical...

To observe the effect of electroacupuncture (EA) on pain behavior, synovial inflammatory response and demyelination saphenous nerve in rats modeled with knee osteoarthritis (KOA) explore mechanism EA for reliving allodynia.Eighty-four male SD were randomly divided into a control group, model group an 28 each one. Intra-articular injection sodium monoiodoacetate (MIA) was administered right joint rat to establish KOA model. In separately, day 5, 7 9 after modeling, applied at "Zusanli" (ST...

The authors conducted this meta-analysis of randomized controlled trials (RCTs) to assess the effects photobiomodulation therapy (PBMT) in treatment androgenic alopecia (AA). RCTs published Pubmed, Web Science, and Embase were systematically reviewed identify PBMT AA patients. outcome measures included hair density growth. Results expressed as weighted mean difference (WMD) with 95% confidence interval (95%CI) a risk ratio (RR) 95%CI. A fixed-effects model or randomized-effects was performed...

OBJECTIVE To compare the effect of electroacupuncture (EA) and transcutaneous electrical acupoint stimulation (TEAS) skin muscle layers Liangqiu (ST34) area on inflammatory muscular pain in rats, so as to study role different-layer afferent nerve fibers acupuncture analgesia. METHODS A total 120 male SD rats were used present study, including 8 for determining excitatory threshold Aδ(Ta) C (Tc) fibers, 48 employed comparing analgesic EA TEAS at intensities Ta Tc, 64 observing TAES...

Skin innervation is an important part of the peripheral nervous system. Although study cutaneous nerve fibers has progressed rapidly, most understanding their distributional and chemical characteristics comes from conventional histochemical immunohistochemical staining on thin tissue sections. With development clearing technique, it become possible to view thicker The present protocol describes multiple fluorescent sections at a thickness 300 µm plantar dorsal skin rat hindfoot, two typical...