A5004354936- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Animal Behavior and Reproduction
- Plant and animal studies
- RNA regulation and disease
- Cell death mechanisms and regulation
- CRISPR and Genetic Engineering
- Insect Utilization and Effects
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Insect and Pesticide Research
- Plant Molecular Biology Research
- Physiological and biochemical adaptations
- Gene Regulatory Network Analysis
- Protein Kinase Regulation and GTPase Signaling
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Hippo pathway signaling and YAP/TAZ
- Protein Tyrosine Phosphatases
- Telomeres, Telomerase, and Senescence
- Antimicrobial Peptides and Activities
- Cellular transport and secretion
- 14-3-3 protein interactions
- Venomous Animal Envenomation and Studies
- Cancer-related Molecular Pathways
Bar-Ilan University
2016-2024
Stanford University
2018-2023
Goodman (Japan)
2020
University of California, San Francisco
2012
Weizmann Institute of Science
2001-2010
Hsp90 is a highly abundant chaperone whose clientele includes hundreds of cellular proteins, many which are central players in key signal transduction pathways and the majority protein kinases. In light variety clientele, mechanism selectivity toward its client proteins major open question. Focusing on human kinases, we have demonstrated that recognizes common surface amino-terminal lobe kinases from diverse families, including two newly identified clients, NFkappaB-inducing kinase...
Toward Addiction can result when substances, such as drugs or alcohol, co-opt the brain's natural reward system. Shohat-Ophir et al. (p. 1351 ; see Perspective by Zars ) explored this potential in Drosophila examining relationship between stimulated mating and unnatural offered ethanol consumption. Males deprived of increased consumption ethanol, and, permitted to mate following deprivation, their decreased. At a mechanistic level, neurotransmitter neuropeptide F (NPF), while deprivation...
Apoptosis is characterized by a translation switch from cap-dependent to internal ribosome entry site (IRES)-mediated protein translation. During apoptosis, several members of the eukaryotic initiation factor (eIF)4G family are cleaved specifically caspases. Here we investigated which caspase-cleaved eIF4G could support cap-independent through IRES elements that retain activity in dying cell. We focused on two major fragments arising cleavage eIF4GI and death-associated 5 (DAP5) proteins...
AbstractThe cellular function of p53 is complex. It well known that plays a key role in response to DNA damage. Moreover, was implicated senescence, and it demonstrated undergoes modification senescent cells. However, not how these modifications affect the ability cells respond To address this question, we studied responses cultured young old normal diploid human fibroblasts variety genotoxic stresses. Young were able undergo p53-dependent p53-independent apoptosis. In contrast, unable...
Death-associated protein kinase is a calcium/calmodulin serine/threonine kinase, which positively mediates programmed cell death in variety of systems. Here we addressed its mode regulation and identified mechanism that restrains apoptotic function growing cells enables activation during death. It involves autophosphorylation Ser<sup>308</sup>within the calmodulin (CaM)-regulatory domain, occurs at basal state, absence Ca<sup>2+</sup>/CaM, inversely correlated with substrate phosphorylation....
Abstract ADAR RNA editing enzymes are high-affinity dsRNA-binding proteins that deaminate adenosines to inosines in pre-mRNA hairpins and also exert editing-independent effects. We generated a Drosophila Adar E374A mutant strain encoding catalytically inactive with CRISPR/Cas9. demonstrate adenosine deamination activity is necessary for normal locomotion prevents age-dependent neurodegeneration. The protein, when expressed at higher than physiological level, can rescue neurodegeneration...
Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is a ubiquitous mechanism that generates transcriptomic diversity. This process particularly important for proper neuronal function; however, little known about how editing dynamically regulated between the many functionally distinct populations of brain. Here, we present spatial map in Drosophila brain and show different possess signatures. After purifying sequencing from genetically marked groups nuclei, identified large...
A-to-I RNA editing is a cellular mechanism that generates transcriptomic and proteomic diversity, which essential for neuronal immune functions. It involves the conversion of specific adenosines in molecules to inosines, are recognized as guanosines by machinery. Despite vast number sites observed across animal kingdom, pinpointing critical understanding their vivo functions remains challenging. Here, we study function an evolutionary conserved site Drosophila , located glutamate-gated...
The death-associated protein (DAP) kinase family includes three kinases, DAP kinase, kinaserelated 1, and ZIP which display 80% amino acid identity within their catalytic domains are functionally linked to common subcellular changes occurring during cell death, such as the process of membrane blebbing.Here we show physical functional cross talk between kinase.The two kinases strong synergistic effects on death when coexpressed physically bind each other via domains.Furthermore,...
The reward system is a collection of circuits that reinforce behaviors necessary for survival [1Kelley A.E. Berridge K.C. neuroscience natural rewards: relevance to addictive drugs.J. Neurosci. 2002; 22: 3306-3311Crossref PubMed Google Scholar, 2Edwards S. Koob G.F. Neurobiology dysregulated motivational systems in drug addiction.Future Neurol. 2010; 5: 393-401Crossref Scopus (115) Scholar]. Given the importance reproduction survival, actions promote successful mating induce pleasurable...
Living in a social environment requires the ability to respond specific stimuli and incorporate information obtained from prior interactions into future ones. One of mechanisms that facilitates interaction is pheromone-based communication. In Drosophila melanogaster, male-specific pheromone cis-vaccenyl acetate (cVA) elicits different responses male female flies, functions modulate behavior context experience-dependent manner. Although it most studied determine complexity response, its...
Abstract Given the current status of coronavirus disease 2019 (COVID-19) as a global pandemic, it is high priority to gain deeper understanding disease's development and how virus impacts its host. Adenosine (A)-to-Inosine (I) RNA editing post-transcriptional modification, catalyzed by ADAR family enzymes, that can be considered part inherent cellular defense mechanism affects innate immune response in complex manner. It was previously reported various viruses could interact with host's...
Living in dynamic environments such as the social domain, where interaction with others determines reproductive success of individuals, requires ability to recognize opportunities obtain natural rewards and cope challenges that are associated achieving them. As such, actions promote survival reproduction reinforced by brain reward system, whereas coping obtaining these is mediated stress-response pathways, activation which can impair health shorten lifespan. While much research has been...
Courtship in Drosophila melanogaster involves a series of innate, complex behaviors that allow male and female flies to exchange sensory information assess the quality potential mate. Although highly robust stereotypical, courtship can be modulated by internal state experience. This protocol describes methods for designing carrying out experiments measure performance single-pair assays, which is paired with female, or competitive presented competitor. It also includes approaches measuring...
We describe a protocol for measuring ethanol self-administration in fruit flies (Drosophila melanogaster) as proxy changes reward states. demonstrate simple way to tap into the fly system, modify experiences related natural reward, and use voluntary consumption measure The approach serves relevant tool study neurons genes that play role experience-mediated of internal state. is composed two discrete parts: exposing rewarding nonrewarding experiences, assaying motivation obtain drug reward....