A5103134351- Monoclonal and Polyclonal Antibodies Research
- Oceanographic and Atmospheric Processes
- Protein purification and stability
- Climate variability and models
- Environmental Toxicology and Ecotoxicology
- Glycosylation and Glycoproteins Research
- Environmental Science and Water Management
- Environmental and Sediment Control
- Tropical and Extratropical Cyclones Research
- Arctic and Antarctic ice dynamics
- Meteorological Phenomena and Simulations
- Viral Infectious Diseases and Gene Expression in Insects
- Fluid Dynamics and Turbulent Flows
- Water Quality and Resources Studies
- Ocean Waves and Remote Sensing
- Atmospheric and Environmental Gas Dynamics
- Toxic Organic Pollutants Impact
- Analytical Chemistry and Chromatography
- Marine and coastal ecosystems
- Methane Hydrates and Related Phenomena
- Geology and Paleoclimatology Research
- Mass Spectrometry Techniques and Applications
- Marine Biology and Environmental Chemistry
- Pharmaceutical and Antibiotic Environmental Impacts
- Marine and environmental studies
Amgen (United States)
2004-2023
James Madison University
2008-2022
University of Washington Medical Center
2022
University of Delaware
2003-2016
Electronic Sensor Technology (United States)
2008
Center Point
2005
Argonne National Laboratory
2004
Stanford University
2004
University of Chicago
2004
Oregon State University
1978-1999
Spectroscopic studies have identified a number of proteins that appear to retain significant residual structure under even strongly denaturing conditions. Intrinsic viscosity, hydrodynamic radii, and small-angle x-ray scattering studies, in contrast, indicate the dimensions most chemically denatured scale with polypeptide length by means power-law relationship expected for random-coil behavior. Here we further explore this discrepancy expanding range characterized denatured-state radii...
An objective measure of the length scale turbulent overturning events, Thorpe scale, L T , is compared to Ozmidov 0 = (ε/ N 3 ) 1/2 where buoyancy frequency and ε kinetic energy dissipation rate. Far from surface in wind‐forced mixing layers seasonal thermocline, are same order, but near a layer, significantly larger than . The change ratio / attributed decrease gradient Richardson number highly energetic zone surface. Another B ( DC x C Cox D molecular diffusivity temperature, order as...
In the accompanying report (Wypych, J., Li, M., Guo, A., Zhang, Z., Martinez, T., Allen, M. Fodor, S., Kelner, D. N., Flynn, G. C., Liu, Y. D., Bondarenko, P. V., Ricci, Dillon, T. and Balland, A. (2008) J. Biol. Chem. 283, 16194–16205), we have identified that human IgG2 subclass exists as an ensemble of distinct isoforms, designated IgG2-A, -B, -A/B, which differ by disulfide connectivity at hinge region. this report, studied structural functional properties isoforms compared them to IgG1....
In this work, we present studies of the covalent structure human IgG2 molecules. Detailed analysis showed that recombinant monoclonal antibody could be partially resolved into structurally distinct forms caused by multiple disulfide bond structures. addition to presently accepted for subclass, also found major structures differ from those documented in current literature. These novel structural isoforms are defined light chain constant domain (C(L)) and heavy C(H)1 covalently linked via...
Observations of vertical temperature microstructure at ocean station P during the mixed layer experiment (Mile) indicate that shape high‐frequency gradient spectrum depends on relative strengths turbulence and stratification. For low Cox number 〈( dT / dz ) 2 〉/〈 〉 linear range Batchelor is not well approximated by observed spectra, while for high a remarkably close correspondence to found. Dissipation rates calculated cutoff wave method show dramatic contrast in between wind speed periods...
The status of the N-terminus proteins is important for amino acid sequencing by Edman degradation, protein identification shotgun and top-down techniques, to uncover biological functions, which may be associated with modifications. In this study, we investigated pyroglutamic formation from N-terminal glutamic residues in recombinant monoclonal antibodies. Almost half antibodies reported literature contain a residue at light or heavy chain. Our reversed-phase high-performance liquid...
Microstructure profiles of temperature, conductivity, and velocity shear during the Arctic Internal Wave Experiment (AIWEX) in March–April 1985 Beaufort Sea are used to investigate thermodynamic processes a diffusive thermohaline staircase. The staircase occurs between depths about 320 430 m, above core relatively warm, salty Atlantic water, where mean temperature salinity increasing with depth. Individual isothermal layers can be tracked for at least several hours, suggesting horizontal...
Spectral analysis of current speed and chlorophyll a measurements in Lake Tahoe, California Nevada, indicates that considerably more variance exists at longer length scales than the speeds. Increasingly, above approximately 100 meters, does not behave as simple passive contaminant distributed by turbulence, which biological processes contribute significantly to observed these large scales.
During the ice camp component of Coordinated Eastern Arctic Experiment (CEAREX) in March–April 1989, 1500 profiles temperature, conductivity, and velocity shear microstructure were obtained near Yermak plateau. The dominant signal isopycnal displacements was diurnal variability plateau, consistent with enhanced currents found this region previously from Fram III IV camps. dissipation rate turbulent kinetic energy ε reached maximum values about 10 −6 W kg −1 two distinct regions, surface...
A new isoform of the light chain a fully human monoclonal immunoglobulin γ2 (IgG2) antibody panitumumab against epidermal growth factor receptor (EGFR) was generated by in vitro aging. The attributed to isomerization aspartate 92 located between phenylalanine 91 and histidine 93 residues antigen-binding region. rate increased with temperature decreased pH. size-exclusion chromatography binding assay used show that one molecule able bind two soluble extracellular EGFR molecules solution, or...
Microstructure measurements in the equatorial Pacific at 140°W late 1984 show a pronounced diurnal variation both high‐frequency internal wave energy and kinetic dissipation rate. Observations indicated that after sunset, waves (presumably generated by convective overturns mixed layer) propagate downward increase turbulence levels pycnocline. It is proposed large layer eddies South Equatorial Current interact with shear caused Undercurrent to generate westward going anisotropic field. The...
Recently, cation exchange chromatography (CEX) using aqueous volatile buffers was directly coupled with mass spectrometry (MS) and applied for intact analysis of therapeutic proteins antibodies. In our study, chemical modifications responsible charge variants were identified by CEX-UV-MS a monoclonal antibody (mAb), bispecific antibody, an Fc-fusion protein. We also report post-CEX column addition organic solvent acid followed mixing at elevated temperatures, which unfolded proteins,...
The glycosylation profile of intact antibody due to the galactose and fucose heterogeneity in N-linked sugars was determined with instrument resolution 5000 10,000. After deconvolution electrospray ionization mass spectra complete convergence, several extra peaks appeared addition observed original spectra. artificial were avoided if stopped after a smaller number iterations. A standard used as an external calibrant minimize measurement errors during long-period experiments. Precision four...
The conventional view of equatorial dynamics requires that the zonal wind stress be balanced, in mean, by vertical integral "large-scale" terms, such as pressure gradient, mesoscale eddy flux, and mean advection, over upper few hundred meters. It is usually presumed surface communicated to interior turbulent processes. Turbulent kinetic energy dissipation rates measured at 140°W during TROPIC HEAT I experiment a production rate–dissipation rate balance argument have been used calculate 30 90...
Tests of a formula derived for the cutoff wave number vertical temperature gradient spectra, using data taken in upper layers North Pacific, show encouraging results. To derive this formula, is assumed to be Batchelor number, with kinetic energy dissipation calculated by combining form used atmosphere calculating eddy diffusivity terms Osborn‐Cox from variance spectrum. Kinetic water column can determined way; profile shows values order 10 −3 cm 2 s at base stormtossed mixing layer. In...