A5077223483- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Electrowetting and Microfluidic Technologies
- Biomedical and Engineering Education
- Genetics, Bioinformatics, and Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Transgenic Plants and Applications
- Microbial Inactivation Methods
- Bacterial Genetics and Biotechnology
- COVID-19 epidemiological studies
- Advanced biosensing and bioanalysis techniques
- Microbial Fuel Cells and Bioremediation
- Gene Regulatory Network Analysis
- Bacteriophages and microbial interactions
- Cell Image Analysis Techniques
- Influenza Virus Research Studies
- Image Processing Techniques and Applications
- bioluminescence and chemiluminescence research
- Vibrio bacteria research studies
- Molecular Biology Techniques and Applications
Georgia Institute of Technology
2022-2024
The Thousand
2023
As the field of synthetic biology expands, need to grow and train science, technology, engineering, math (STEM) practitioners is essential. However, lack access hands-on demonstrations has led inequalities opportunity practice. In addition, there a gap in providing content that enables students make their own bioengineered systems. To address these challenges, we develop four shelf-stable cell-free biosensing educational modules work by simply adding water DNA freeze-dried crude extracts...
The centrifuge is an essential tool for many aspects of research and medical diagnostics. However, conventional centrifuges are often inaccessible outside standard laboratory settings, such as remote field sites, because they require a constant external power source can be prohibitively costly in resource-limited settings Science, technology, engineering, mathematics (STEM)-focused programs. Here we present the 3D-Fuge, 3D-printed hand-powered centrifuge, novel alternative to benchtop...
Bacterial biosensors can enable programmable, selective chemical production, but difficulties incorporating metabolic pathways into complex sensor circuits have limited their development and applications. Here we overcome these challenges present the of fast-responding, tunable cells that produce different pigmented metabolites based on extracellular concentrations zinc (a critical micronutrient). We create a library dual-input synthetic promoters decouple cell growth from zinc-specific...
Electroporation is a basic yet powerful method for delivering small molecules (RNA, DNA, drugs) across cell membranes by application of an electrical field. It used many diverse applications, from genetically engineering cells to drug- and DNA-based vaccine delivery. Despite this broad utility, the high cost electroporators can keep approach out reach budget-conscious laboratories. To address need, we develop simple, inexpensive, handheld electroporator inspired derived common household...
The centrifuge is an essential tool for many aspects of research and medical diagnostics. However, conventional centrifuges are often inaccessible outside laboratory settings, such as remote field sites, require a constant external power source, can be prohibitively costly in resource-limited settings STEM-focused programs. Here we present the 3D-Fuge, 3D-printed hand-powered centrifuge, novel alternative to standard benchtop centrifuges. Based on design principles paper-based this...
Abstract As the field of synthetic biology expands, need to grow and train science, technology, engineering, math (STEM) practitioners is essential. However, lack access hands-on demonstrations has led inequalities opportunity practice. In addition, there a gap in providing content that enables students make their own bioengineered systems. To address these challenges, we develop four shelf-stable cell-free biosensing educational modules work by just-adding-water DNA freeze-dried crude...
High-cost DNA extraction procedures pose significant challenges for budget-constrained laboratories. To address this, we introduce OpenCell, an economical, open-source, 3-in-1 laboratory device that combines the functionalities of a bead homogenizer, microcentrifuge, and vortex mixer. OpenCell utilizes modular attachments magnetically connect to central rotating brushless motor. This motor couples epicyclic gearing mechanism, enabling efficient homogenization, mixing, centrifugation within...
Electroporation is a basic yet powerful method for delivering small molecules (RNA, DNA, drugs) across cell membranes by application of an electrical field. Due to its vital role, electroporation has wide applicability from genetically engineering cells, drug- and DNA-based vaccine delivery. Despite broad applications in biological research, the high cost electroporators obstacle many budget-conscious laboratories. To address this need, we describe simple, inexpensive, hand-held...
The ongoing Yemen cholera outbreak has been deemed one of the worst outbreaks in history, with over a million people impacted and thousands dead. Triggered by civil war, shaped various political, environmental, epidemiological factors continues to worsen. While several effective treatments, untimely inefficient distribution existing medicines primary cause mortality. With hope facilitating resource allocation, mathematical models have created track Yemeni identify at-risk administrative...
Abstract Microplate readers are laboratory instruments used to measure biological properties and reactions within a microplate. A microplate consists of small wells in which separated take place. These convert the progression biochemical processes into optical signals. reader detects these signals quantifies parameter interest. Most lab-grade can cost up $20,000, making them unaffordable for underfunded labs developing countries. In light this, we developed Plate-Q, $150 frugal capable...
High-cost DNA extraction procedures pose significant challenges for budget-constrained laboratories. To address this, we introduce OpenCell, an economical, open-source, 3-in-1 laboratory device that combines the functionalities of a bead homogenizer, microcentrifuge, and vortex mixer. OpenCell utilizes modular attachments magnetically connect to central rotating brushless motor. This motor couples epicyclic gearing mechanism, enabling efficient homogenization, mixing, centrifugation within...
Q5 reaction buffer-5ul dNTPs-1ulPrimer mix-2.5ul(1.25ul per primer) High Fidelity Polymerase-.