A5060741497- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Cancer Cells and Metastasis
- Skin and Cellular Biology Research
- Nuclear Structure and Function
- Cancer Genomics and Diagnostics
- Tissue Engineering and Regenerative Medicine
- Nanofabrication and Lithography Techniques
- Cell Image Analysis Techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Nanoplatforms for cancer theranostics
- Collagen: Extraction and Characterization
- Graphene and Nanomaterials Applications
- Wound Healing and Treatments
- Additive Manufacturing and 3D Printing Technologies
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
Center for Cancer Research
2024-2025
Harvard University
2024-2025
Massachusetts General Hospital
2024-2025
Middle East Technical University
2018-2023
Abstract Circulating Tumor Cells (CTCs) in blood encompass DNA, RNA, and protein biomarkers, but clinical utility is limited by their rarity. To enable tumor epitope-agnostic interrogation of large volumes, we developed a high-throughput microfluidic device, depleting hematopoietic cells through high-flow channels force-amplifying magnetic lenses. Here, apply this technology to analyze patient-derived leukapheresis products, interrogating mean volume 5.83 liters from seven patients with...
E3 ubiquitin ligases mediating turnover of proteins engaged in cancer progression point to key regulatory nodes. To uncover modifiers metastatic competency, we conducted an vivo genome-wide CRISPR-inactivation screen using cultured breast circulating tumor cells, following intravascular seeding and lung colonization. We identified HECTD4, a previously uncharacterized gene encoding conserved potential HECT domain-containing transferase, as potent metastasis suppressor. show that purified...
Abstract E3 ubiquitin ligases mediating turnover of proteins engaged in cancer progression point to key regulatory nodes. To uncover modifiers metastatic competency, we conducted an vivo genome-wide CRISPR-inactivation screen using cultured breast circulating tumor cells, following intravascular seeding and lung colonization. We identified HECTD4, a previously uncharacterized gene encoding conserved potential HECT domain-containing transferase, as potent suppressor. show that purified HECTD4...
Abstract Cell‐substrate interactions involve constant probing of microenvironment by cells. One the responses cells to environmental cues is change conformation their cytoplasm and nucleus. We hypothesized that surface chemistry topography could be engineered make these differences significant enough. When designing substrates would accentuate differences, we prepared surfaces carrying cell adhesive biological arranged in specific patterns. Collagen type I poly(lactic acid‐ co ‐glycolic...
Mechanotransduction proteins transfer mechanical stimuli through nucleo-cytoskeletal coupling and affect the nuclear morphology of cancer cells. However, contribution actin filament integrity has never been studied directly. It is hypothesized that differences in deformability cells are influenced by filaments. In this study, transparent micropatterned surfaces as simple tools to screen cytoskeletal distortions presented. Surfaces decorated with micropillars used culture image breast...
We produced a novel three-dimensional (3D) bone tumor model (BTM) to study the interactions between healthy and cells in microenvironment, migration tendency of cells, efficacy an anticancer drug, Doxorubicin, on cancer cells. The consisted two compartments: (a) tissue mimic, made poly(lactic acid-co-glycolic acid) (PLGA)/beta-tricalcium phosphate (β-TCP) sponge seeded with human fetal osteoblastic (hFOB) umbilical vein endothelial (HUVECs), (b) lyophilized collagen osteosarcoma (Saos-2)....
In the recent years, microfabrication technologies have been widely used in cell biology, tissue engineering, and regenerative medicine studies. Today, implementation of microfabricated devices cancer research is frequent advantageous because it enables study cells controlled microenvironments provided by microchips. Breast one most common cancers women, way breast interact with their physical microenvironment still under investigation. this study, we developed a transparent culture chip...
Breast cancer is a heterogeneous and dynamic disease, in which cells are highly responsive to alterations the microenvironment. Today, conventional methods of detecting give rather static image condition so properties such as invasiveness metastasis difficult capture. In this study, molecular-level evaluations patients with breast adenocarcinoma were combined vitro on micropatterned poly(methyl methacrylate) (PMMA) biomaterial surfaces that deform cells. A correlation between deformability...
ABSTRACT The mitotic inhibitor docetaxel (DTX) is often used to treat endocrine-refractory metastatic breast cancer, but initial responses are mitigated as patients eventually have disease progression. Using a cohort of ex vivo cultures circulating tumor cells (CTCs) from with heavily pretreated cancer (n=18), we find two distinct patterns DTX susceptibility, independent clinical treatment history. In CTCs cultured some patients, single dose results in complete cell killing, associated...
Abstract Circulating Tumor Cells (CTCs), interrogated by sampling blood from patients with cancer, contain multiple analytes, including intact RNA, high molecular weight DNA, proteins, and metabolic markers. However, the clinical utility of tumor cell-based liquid biopsy has been limited since CTCs are very rare, current technologies cannot process volumes required to isolate a sufficient number cells for in-depth assays. We previously described high-throughput microfluidic prototype...
Abstract Circulating Tumor Cells (CTCs) in blood containing a complete set of single-cell analytes, including high-molecular-weight DNA, intact RNA, and both intracellular cell-surface proteins serve as promising tool cancer diagnosis prognosis through liquid biopsy. However, their clinical utility has been limited by low concentration inadequate enrichment technology. Diagnostic leukapheresis holds promise for isolating sufficient CTCs, but enriching ultra-rare cells from large, complex...
Abstract We produced a three dimensional (3D) bone tumor model (BTM) to study the interactions between healthy and cells in tissue microenvironment, migration of efficacy an anticancer drug, Doxorubicin, for personalized medicine applications. The consisted two compartments: (a) mimic, poly(lactic acid-co-glycolic acid) (PLGA)/beta-tricalcium phosphate (β-TCP) sponge that was seeded with human fetal osteoblastic (hFOB) umbilical vein endothelial (HUVECs), (b) lyophilized collagen...
Abstract It is well known that chemical and biomechanical interactions between the nucleus cytoskeleton are involved in critical for movement, migration nuclear positioning of cancer cells. Through nucleo-cytoskeletal coupling, proteins LINC complex envelope capable transducing cytoplasmic mechanical input across membrane; however, their functional importance behavior cells nuclei has never been directly tested. In this study, our assumption was differences malignancies breast result...