Polycystic ovary syndrome (PCOS) is a common endocrine disorder characterized by insulin resistance and hyperandrogenism. The interaction of these factors might result in increased risks miscarriage pregnancy complications such as gestational diabetes mellitus (GDM). To examine the women with PCOS, we compared obstetrical outcomes between patients without PCOS. We also studied differences maternal characteristics, glucose intolerance PCOS GDM, obesity, successful pregnancies miscarriages....

Abstract We describe an exceptionally rare case of epithelioid trophoblastic tumor (ETT) in a patient with recurrent implantation failure (RIF). A 38‐year‐old Japanese woman, gravida 1, para was diagnosed RIF following three unsuccessful cryopreserved morphologically good embryo transfers hormone replacement cycles after thawing. She underwent hysteroscopy and endometrial biopsy to exclude chronic endometritis but referred because ETT strongly suspected. Following preoperative diagnosis...

It is established that neuronal NO synthase (nNOS) associated with the chaperone hsp90, although functional role for this interaction has not been defined. We have discovered inhibition of hsp90 by radicicol or geldanamycin nearly prevents heme-mediated activation and assembly heme-deficient apo-nNOS in insect cells. This effect concentration-dependent over 75% achieved at 20 microm radicicol. The ferrous carbonyl complex nNOS formed when inhibited, indicating heme insertion prevented....

Guanabenz, a metabolism-based irreversible inactivator of neuronal nitric-oxide synthase (nNOS) in vitro, causes the loss immunodetectable nNOS vivo. This process is selective that slowly reversible inhibitor N G-nitro-l-arginine did not decrease levels To better understand mechanism for protein vivo, we have investigated effects guanabenz andN HEK 293 cells stably transfected with enzyme. We show here guanabenz, but G-nitro-l-arginine, caused inactivation and cells. In studies cycloheximide...

It is established that neuronal NO synthase (nNOS) ubiquitinated and proteasomally degraded. The metabolism-based inactivation of nNOS the inhibition heat shock protein 90 (hsp90)-based chaperones, which are known to regulate nNOS, both lead enhanced proteasomal degradation nNOS. mechanism this selective proteolytic degradation, or in essence how becomes labilized recognized for ubiquitination subsequent has not been determined. In current study, we used a crude preparation reticulocyte...

Although risk factors for cardiovascular disease, such as obesity, hyperinsulinemia, and dyslipidemia, are commonly observed in women with polycystic ovary syndrome (PCOS), impairment of vascular function is still controversial. We evaluated the young PCOS.We arterial stiffness 54 PCOS 24 healthy control using a volume-plethysmographic apparatus equipped multi-element applanation tonometry sensor left common carotid artery studied correlations various factors.There was no significant...

Isosorbide dinitrate (ISDN), a nitric oxide donor, was applied transdermally for 4–16 days to 4 preeclamptic women with oligohydramnios, intrauterine fetal growth retardation (IUGR), and elevated resistance of blood flow in the uterine arteries. Pulsed Doppler ultrasonography revealed immediate drastic improvement pulsatility index (PI) arteries following treatment ISDN. The average PI patients reduced approximately 67% that untreated state. In 2 amniotic fluid gradually increased over few...

Because overproduction of nitric oxide (NO) and peroxynitrite is known to cause tissue injury, the expression NO synthases (NOS) generation were investigated in adenomyosis. Immunoreactivities endothelial inducible NOS demonstrated phase-dependent changes normal endometrium, eutopic endometrium However, expressed throughout menstrual cycle ectopic from majority patients with Nitrotyrosine, a footprint peroxynitrite, was detected concomitantly protein. This suggested that high doses...

Background. Risk factors for cardiovascular disease, including chronic anovulation, obesity, hyperandrogenism, hyperinsulinemia, and dyslipidemia, are commonly observed in women with polycystic ovary syndrome (PCOS). We evaluated uterine perfusion its correlation clinical biochemical parameters PCOS. Methods. performed a pulsed Doppler study on arterial blood flow 25 PCOS 45 control regular menstrual cycles. was diagnosed based oligomenorrhea, ovaries determined by means of ultrasonography,...

Autonomic nervous system activity was studied to evaluate the physical and mental state of women with unexplained recurrent pregnancy loss (RPL).Heart rate variability (HRV) is a measure beat-to-beat temporal changes in heart provides indirect insight into autonomic tone can be used assess sympathetic parasympathetic tone. We by measuring HRV 100 RPL 61 healthy female volunteers as controls. The degree distress assessed using Kessler 6 (K6) scale.The K6 score significantly higher than...

Abstract Aim: Male‐to‐female (MTF) transsexuals are treated with estrogen and without progestin through a variety of routes. The aim this study is to evaluate the arterial stiffness in MTF undergoing hormonal treatment. Methods: We evaluated 156 (22 untreated 129 only or plus progestin) using volume‐plethysmographic apparatus equipped multi‐element applanation tonometry sensor. Results: parenteral were significantly older than transsexuals. Hematocrit, uric acid activated partial...

We studied the effects of advanced glycation end products (AGEs), which are known to accumulate in patients with diabetes, autoimmune diseases, or those who smoke, on embryonal development. Pronuclear (PN) embryos were obtained by flushing fallopian tubes rats after superovulation and mating. The cleavage rate blastocyst yield evaluated at 24, 72, 96, 120 h culture. Glyoxal, an AGE-forming aldehyde, suppressed development every stage from PN a concentration-dependent manner. embryo was also...

It has been shown that administration of cigarette smoke to rats leads loss neuronal nitric-oxide synthase (nNOS) activity and nNOS protein in penile tissue. The exact mechanism for this is not known. In the current study, we investigated whether extracts prepared from or itself could directly inhibit activity. We discovered extract cause a time-, concentration-, calmodulin-dependent inactivation an vitro system containing purified enzyme. _L-Arginine, but _D-arginine,...

Abstract Dienogest is a fourth‐generation progestin that used for the treatment of endometriosis. We report case premenstrual mood changes in patient with schizophrenia who was unresponsive to conventional but successfully managed dienogest. A 37‐year‐old Japanese woman referred our hospital and diagnosed exacerbation or coexisting dysphoric disorder schizophrenia. She had already taken maximal doses selective serotonin reuptake inhibitors combined oral contraceptives produced intolerable...

It is established that<i>N</i>^G-amino-l-arginine (NAA) a metabolism-based inactivator of all three major nitric-oxide synthase (NOS) isoforms. The mechanism by which this inactivation occurs, however, not well understood. In the current study, we discovered that neuronal isoform NOS (nNOS) NAA in vitro results covalent alteration heme prosthetic group, part, to products contain an intact porphyrin ring and are either dissociable from or irreversibly bound protein. concomitant with...

Vascular dysfunction has been reported in women with recurrent pregnancy loss (RPL). We investigated the severity of vascular non-pregnant RPL and its correlation anti-heat shock protein (HSP) antibodies that are known to induce arteriosclerosis. measured serum anti-HSP60 antibodies, anti-HSP70 anti-phospholipid (APA) 68 29 healthy controls. Among RPL, 14 had a diagnosis antiphospholipid syndrome (APS), remaining 54, causes for were unexplained. Compared controls, brachial-ankle pulse wave...