A5052795970- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Ion Channels and Receptors
- T-cell and B-cell Immunology
- Phytochemicals and Antioxidant Activities
- Cancer Immunotherapy and Biomarkers
- Polyamine Metabolism and Applications
- Hematopoietic Stem Cell Transplantation
- Immunodeficiency and Autoimmune Disorders
- Pain Mechanisms and Treatments
- Global Cancer Incidence and Screening
- Pluripotent Stem Cells Research
- Biochemical Analysis and Sensing Techniques
- Cancer Risks and Factors
- Sulfur Compounds in Biology
- Renal and related cancers
- Nutritional Studies and Diet
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Virus-based gene therapy research
- Sympathectomy and Hyperhidrosis Treatments
- Botulinum Toxin and Related Neurological Disorders
- Neurobiology and Insect Physiology Research
- Migraine and Headache Studies
- Bioactive Compounds and Antitumor Agents
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2024
Policlinico San Matteo Fondazione
2014-2024
Organ Transplantation and Immunology Institute
2017
Casa Sollievo della Sofferenza
2015
Colorectal carcinoma (CRC) represents the fourth most common cancer worldwide and is third cause of malignancy-associated mortality. Distant metastases to liver lungs are main drivers CRC-dependent death. Pro-oxidant therapies, which halt disease progression by exacerbating oxidative stress, represent an antitumour strategy that currently exploited chemotherapy ionizing radiation. A more selective therapeutically exploit reactive oxygen species (ROS) signaling would consist in targeting a...
// Estella Zuccolo 1, * , Umberto Laforenza 2, Federica Ferulli 3, Giorgia Pellavio 2 Scarpellino 1 Matteo Tanzi 3 Ilaria Turin Pawan Faris 4 Angela Lucariello 5 Marcello Maestri 6 Dlzar Ali Kheder 7 Germano Guerra Paolo Pedrazzoli 8 Daniela Montagna 9, ** and Francesco Moccia Department of Biology Biotechnology "Lazzaro Spallanzani", University Pavia, Italy Molecular Medicine, Laboratory Immunology Transplantation, Foundation IRCCS Policlinico San Matteo, Biology, College Science,...
Treatment of advanced metastatic colorectal cancer (mCRC) patients is associated with a poor prognosis and significant morbidity. Moreover, targeted therapies such as anti-epidermal growth factor receptor (EGFR) have no effect in tumors harboring mutation the RAS gene. The failure conventional treatment to improve outcomes mCRC has prompted development adoptive immunotherapy approaches including natural killer (NK)-based therapies. In this study, after confirmation that patients’ NK cells...
Obtaining human tumor cell lines from fresh tumors is essential to advance our understanding of antitumor immune surveillance mechanisms and develop new ex vivo strategies generate an efficient anti-tumor response. The present study delineates a simple rapid method for efficiently establishing primary cultures starting samples different types, while maintaining the immuno-histochemical characteristics original tumor. We compared two disaggregate specimens. After short or long term in vitro...
Exogenous administration of hydrogen sulfide (H2S) is emerging as an alternative anticancer treatment. H2S-releasing compounds have been shown to exert a strong effect by suppressing proliferation and/or inducing apoptosis in several cancer cell types, including colorectal carcinoma (CRC). The mechanism whereby exogenous H2S affects CRC yet be clearly elucidated, but it could involve increase intracellular Ca2+ concentration ([Ca2+]i). Herein, we sought assess for the first time whether (and...
Advanced therapy medicinal products (ATMPs) are novel drugs based on genes, cells or tissues developed to treat many different diseases. Stability studies of each new ATMP need be performed define its shelf life and guarantee efficacy safety upon infusion, these presently guidelines originally drafted for standard pharmaceutical drugs, which have properties stored in conditions quite from cell products. The aim this report is provide evidence-based information stability ATMPs that will...
(1) Background: Store-operated Ca2+ entry (SOCE) drives the cytotoxic activity of T lymphocytes (CTLs) against cancer cells. However, SOCE can be enhanced in cells due to an increase expression and/or function its underlying molecular components, i.e., STIM1 and Orai1. Herein, we evaluated tumour-infiltrating (TILs) from metastatic colorectal (mCRC) patients. (2) Methods: Functional studies were conducted TILs expanded ex vivo CRC liver metastases. Peripheral blood healthy donors (hPBTs)...
The limited efficacy of Natural Killer (NK) cell-based immunotherapy results in part from the suboptimal expansion and persistence infused cells. Recent reports suggest that generation NK cells with memory-like properties upon vitro activation defined cytokines might be an effective way ensuring long-lasting cell function vivo. Here, we demonstrate IL-12, IL-15 IL-18 followed by a one-week culture optimal doses Interleukin (IL-2) generates substantial numbers able to persist for at least...
A number of studies have evidenced the effect modifiable lifestyle factors such as diet, breastfeeding and nutritional status on breast cancer risk. However, none addressed missing data problem in epidemiologic research South America. Missing is a frequent epidemiological settings general. Estimates obtained from these may be biased, if no appropriate method for handling applied. We performed Multiple Imputation values covariates case-control study Córdoba (Argentina) to optimize risk...
Methods We reviewed the charts of 134 patients treated with OnabotulinumtoxinA who received up to 9 cycles. Patients were injected regularly a 3-month (±10 days) interval. They assessed for headache days and hours, intensity pain by Visual Analogue Scale (VAS), number any acute drug intake. Photophobia, phonophobia, osmophobia, nausea as well. The results also analysed based on CM onset.