In this article, we present an overview of milkfish (Chanos chanos) culture, in order to provide basic information on its life history, reproductive biology, fry production, culture methods, and genetics as well commercial importance. Milkfish is a warm-water marine species, widespread the Asian-Pacific region; it unique species family Chanidae. Currently important brackish water aquaculture Southeast Asia represents component fisheries sector national economy Indonesia, Philippines, Taiwan;...

The Pacific bluefin tuna, Thunnus orientalis, is distributed mainly in the north Ocean, but a few individuals are also found south Ocean. We tested ‘one-stock’ hypothesis that genetic differentiation absent between populations from and Oceans. Three molecular markers, cytochrome (Cyt) b, control region (CR) microsatellites, were applied to identify species, investigate population structure infer demographics of T. orientalis Taiwanese New Zealand waters. Tissue samples collected (n = 53) 70)...

Morphometric and genetic data were used to compare two sympatric morphologically similar species, Acanthopagrus berda taiwanensis , in Dapeng Bay, South‐western Taiwan. A principle component analysis of morphological indicated a distinction between the with pectoral fin length eye diameter accounting for 32·27% variation. Interspecific sequence divergence, based on mtDNA cytochrome b (0·118 ± 0·01), was larger than intraspecific divergences haplotypes (0·007 A. 0·003 ). Individuals species...

Onychostoma barbatulum and O. alticorpus, two primarily freshwater cyprinid fish, have similar morphological characters partially overlapping ecological habitats. In order to explore the genetic differences between these species, chromosomal characteristics variations were examined by fluorescence in situ hybridization (FISH) of 5S rDNA cytochrome (Cyt) b gene analysis. Ten specimens alticorpus collected from Nanzihsian Stream southern Taiwan. FISH revealed that loci found at a...

Anguilla eels are divided into temperate and tropical eels, based on their major distributions. The present study collected two japonica anguilla, marmorata bicolor pacifica, to examine questions: do have different genetic polymorphic patterns?; anguilla a closer relationship each other than eels? In total, 274 sequences were cloned sequenced from six conserved microsatellite loci patterns of these four catadromous eels. Different mutational events, including substitutions, repeat-unit...

Thirty clones of a highly repetitive HindIII fragment DNA from seven populations Acrossocheilus paradoxus (Cyprinidae) were isolated and sequenced. The represents tandemly repeated sequence, with monomeric unit 270 bp, amounting to 0.08-0.10% the fish genome. Higher units this monomer appear as ladder in Southern blots. satellite family is conserved three genera Cyprinidae. Variation nucleotide sequences fragment, which A+T-rich, distributed both within individuals among populations. High...

Mei-Chen Tseng, Dian-Hao Yang, and Tsair-Bor Yen (2017)Onychostoma barbatulum has become an aquaculture species with high economic value in Taiwan. It was observed that females can grow faster to a larger size than males on farms. Therefore, development of feminized fry increase farm profits the future. The purpose this study establish optimal incubation, feed, feminization temperatures produce ratio better survival rates for O. fry. performance mode sex-determination by temperature also...

The catadromous Platyeriocheir formosa is a crab endemic in Taiwan. To conserve P. population diversity and ensure the sustainable use of this natural resource, we have developed new genetic markers, 17 polymorphic microsatellite loci, to promote study its genetics future. In study, more than 70 sequences were found. Among these, 18 loci selected analyze formosa. With exception Pfo15 locus, all remaining with allelic numbers ranging from 3-14. Heterozygosity within ranged 0.2-0.95 an average...

Abstract Acanthopagrus berda (Sparidae) is abundant in estuaries and inshore waters of the tropical Indo‐Pacific oceans. Because drastic decrease species’ wild populations, there an urgent need to understand its population structure. Eight microsatellite loci were isolated from A. . An analysis 50 individuals Tapeng Lagoon, southwestern Taiwan demonstrated a low degree diversity. The allelic numbers ranged nine 20. observed heterozygosity ( H O ) 0.28 0.64 (mean, 0.45). We also investigated...

A Hin dIII repetitive DNA family from Acrossocheilus paradoxus , a cyprinid fish endemic to Taiwan, was isolated and identified as tandem arrangement of satellites in the genomic DNA. Cross‐hybridization revealed similar patterns across genera two families suggested that this is conserved satellite sequence fish. Forty‐five monomeric repeat units were cloned sequenced, found be approximately 210 base pairs long have an average composition 52·8% A+T. Alignment analysis by examining 45 strands...

Yan-Horn Lee, Tsair-Bor Yen, Chiu-Fen Chen, and Mei-Chen Tseng (2018)Thunnus tunas in Scombridae are divided into the temperate subgenus Thunnus (bluefin group) tropical Neothunnus (yellowfin species based on anatomic traits distributions. The main purpose of this study was to examine systematic status T. obesus karyotype, cytochrome (Cyt) b gene, 5S ribosomal DNA sequences. All obesus, albacares, alalunga, orientalis specimens were caught southeastern coastal waters off island Taiwan....

The cobia Rachycentron canadum, mainly distributed in the warm waters of tropical and subtropical regions around world, remains a fish considerable economic importance. Detailed diversity number microsatellite sequences genome are still unintelligible. primary aim this work was to identify quantify miscellaneous SSR genome. More than 280,000 were sequenced screened using next-generation sequencing technology identification. Perfect mononucleotide repeats, dinucleotide microsatellites,...

Chiao-Chuan Han, Tsair-Bor Yen, Nian-Cih Chen, and Mei-Chen Tseng (2017) Both Onychostoma barbatulum O. alticorpus are primary freshwater fish in Taiwan. The former has been developed as an aquaculture species with high economic value, while the latter is a native endemic Understanding cytogenetic information of these two necessary for their selected breeding, recovery, management. In this study, Giemsa staining, silver-binding nucleolar organizer region (Ag-NOR), C-banding, fluorescence...