A5083333830- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Nutrition, Genetics, and Disease
- Cell Image Analysis Techniques
- Data Analysis with R
- Skin and Cellular Biology Research
- Genetics, Bioinformatics, and Biomedical Research
- Thermal Regulation in Medicine
- Health and Medical Research Impacts
- Artificial Intelligence in Healthcare and Education
- Radiology practices and education
- Ultrasound in Clinical Applications
- Biomedical Text Mining and Ontologies
- COVID-19 diagnosis using AI
- Ultrasound and Hyperthermia Applications
- Autopsy Techniques and Outcomes
- Mobile Health and mHealth Applications
- Obesity and Health Practices
- Modular Robots and Swarm Intelligence
- Robot Manipulation and Learning
- T-cell and B-cell Immunology
- Advanced Fluorescence Microscopy Techniques
- Cytomegalovirus and herpesvirus research
- Clinical Reasoning and Diagnostic Skills
- Immune Response and Inflammation
Madigan Army Medical Center
2020-2024
University of Iowa
2024
United States Department of the Army
2020
United States Army
2020
Washington State University
2014-2019
Abstract Integrins are components of cell-matrix adhesions, and function as scaffolds for various signal transduction pathways. So far no lipid ligand integrin has been reported. Here we show that a lipid, oxysterol 25-hydroxycholesterol (25HC), directly binds to α5β1 αvβ3 integrins activate integrin-focal adhesion kinase (FAK) signaling. Treatment macrophages epithelial cells with 25HC results in an increase activated podosome focal matrix sites. Moreover, activation pattern recognition...
We find that clusters of β4 integrin, organized into distinct puncta, localize along vimentin filaments within lamellipodia at the cell edge A549 cells, as assessed by interferometric photoactivated localization microscopy. Moreover, puncta and exhibit a dynamic interplay in live viewed structured-illumination microscopy, with integrin associate persisting for longer than those do not. Interestingly, cells regulates cytoskeleton organization. When is knocked down there loss from...
α6β4 integrin is localized in a unique punctate distribution at the cell-substratum interface along leading front of single, front-rear-polarized A549 cells. These puncta are interspersed between focal adhesions and lack association with actin cytoskeleton. Knockdown β4 cells inhibits their directed migration, knockdown exhibiting large reduced dynamics. Despite these changes, speed equivalent to control Interestingly, such cells, α6 retains its distribution. Moreover, we observe loss β1...
During wound healing of the skin, keratinocytes disassemble hemidesmosomes and reorganize their actin cytoskeletons in order to exert traction forces on move directionally over dermis. Nonetheless, transmembrane hemidesmosome component collagen XVII (ColXVII) is found actin-rich lamella, situated behind lamellipodium. A set bundles, along which ColXVII colocalizes with actinin4, present at each lamella. Knockdown either or actinin4 not only inhibits directed migration but also relieves...
A biorepository, also referred to as a “biobank,” is collection of biologic samples that are stored for laboratory research. With the emergence precision medicine, importance leveraging individual patient biomolecular signatures improve diagnosis, prognosis, and treatment becoming increasingly recognized. Successful development sustainment biorepository provides potential transformative preclinical Establishing biobank requires team approach with involvement institutions' research regulatory...
<ns4:p>Super resolution imaging is becoming an increasingly important tool in the arsenal of methods available to cell biologists. In recognition its potential, Nobel Prize for chemistry was awarded three investigators involved development super 2014. The availability commercial instruments has further spurred new and reagents designed take advantage techniques. Super offers advantages traditionally associated with light microscopy, including use gentle fixation specimen preparation methods,...
This cross-sectional study assesses the publication productivity of graduating general surgery residents as well factors associated with a chief resident’s academic research output.
Are We Speaking the Same Language? Communicating Diagnostic Probability in Radiology ReportMatthew D. Grant, MD1,2, Corey R. O'Reilly, MD1, Joshua Ladner, Lisa Mack, MD3, Andrew H. McGrain, Zachary T. Colburn, PhD4 and Brian M. Boldt, DO1,2Audio Available | Share
Objectives Sonography is routinely performed in obstetrics. For safety purposes, intensity‐output displays are included all ultrasound machines. There few studies on provider understanding of safety. We sought to assess obstetrics providers' level knowledge biosafety before and after educational intervention. Methods This a cross‐sectional study using survey research examine the providers performing sonography. The faculty, fellows, residents gynecology family medicine, certified nurse...
Abstract Modern biomedical image analyses workflows contain multiple computational processing tasks giving rise to problems in reproducibility. In addition, datasets can span both spatial and temporal dimensions, with additional channels for fluorescence other data, resulting that are too large be processed locally on a laptop. For omics analyses, software containers have been shown enhance reproducibility, facilitate installation provide access scalable resources the cloud. However, most...
<sec> <title>BACKGROUND</title> The rise of text-bots such as Chat Generative Pre-Trained Transformer prompts scrutiny artificial intelligence (AI) in graduate medical education (GME), where data on provider and trainee views AI's appropriateness governance remain sparse. </sec> <title>OBJECTIVE</title> To identify common perceptions concerns about among GME trainees faculty characterize differences opinions. <title>METHODS</title> In 2023, we conducted a cross-sectional Qualtrics™-based...
After radiologists perform a set of chest-x-rays (CXRs) they write short report describing their observations and interpretations. Because these reports are free-text documents, there is the risk miscommunication, which can result in reduced patient outcomes. We applied text mining methods to radiology MIMIC Chest X-ray (MIMIC-CXR) database [5], consisting 227,835 de-identified reports. selected relevant terms (features) developed predictive models that take as input return probability...
ABSTRACT Introduction Metabolic syndrome (MetS) is a threat to the active component military as it impacts health, readiness, retention, and cost Military Health System. The most prevalent risk factors documented in service members’ health records are high blood pressure (BP), low high-density lipoprotein cholesterol, elevated triglycerides. Other include abdominal obesity fasting glucose. Precision nutrition counseling wellness software applications have demonstrated positive results for...