ADVERTISEMENT RETURN TO ISSUEPREVViewpointNEXTEngineering of Flexible Loops in EnzymesBettina M. Nestl and Bernhard Hauer*View Author Information Institute Technical Biochemistry, University Stuttgart, Allmandring 31, 70569 Germany*E-mail: [email protected]Cite this: ACS Catal. 2014, 4, 9, 3201–3211Publication Date (Web):August 8, 2014Publication History Received12 March 2014Published online19 August inissue 5 September...

Abstract Reducing reactions are among the most useful transformations for generation of chiral compounds in fine‐chemical industry. Because their exquisite selectivities, enzymatic approaches have emerged as method choice reduction CO and activated CC bonds. However, stereoselective CN bonds is still its infancy—it was only recently described after discovery enzymes capable imine reduction. In our work, we increased spectrum imine‐reducing by database analysis. By combining currently...

Abstract Imine reductases (IREDs) have emerged as promising biocatalysts for the synthesis of chiral amines. In this study, asymmetric imine reductase‐catalyzed intermolecular reductive amination with NADPH hydrogen source was investigated. A highly chemo‐ and stereoselective reductase applied by using a panel carbonyls different amine nucleophiles. Primary secondary products were generated in moderate to high yields enantiomeric excess values. The formation intermediate studied between...

CYP153A from Marinobacter aquaeolei has been identified as a fatty acid ω-hydroxylase with broad substrate range. Two hotspots predicted to influence specificity and selectivity were exchanged. Mutant G307A is 2- 20-fold more active towards acids than the wild-type. Residue L354 determinant for enzyme ω-regioselectivity.

The oxofunctionalization of saturated hydrocarbons is an important goal in basic and applied chemistry. Biocatalysts like cytochrome P450 enzymes can introduce oxygen into a wide variety molecules very selective manner, which be used for the synthesis fine bulk chemicals. Cytochrome from CYP153A subfamily have been described as alkane hydroxylases with high terminal regioselectivity. Here we report product yields resulting C(5)-C(12) alcohol oxidation catalyzed by Mycobacterium marinum...

Summary A bacterial P 450 monooxygenase‐based whole cell biocatalyst using Escherichia coli has been applied in the production of ω‐hydroxy dodecanoic acid from ( C12‐FA ) or corresponding methyl ester. We have constructed and purified a chimeric protein where fusion monooxygenase CYP153A M arinobacter aquaeloei to reductase domain BM3 B acillus megaterium ensures optimal expression efficient electron coupling. The chimera was demonstrated be functional three times more than other sets redox...

Abstract Die Verwendung von Enzymen als Katalysatoren zur Synthese neuartiger Verbindungen ist in den letzten Jahren zunehmend Fokus gerückt. Entsprechend hohe Erwartungen werden an die Entdeckung und Identifizierung neuer Biokatalysatoren für organische gestellt. Dies spiegelt sich auch der Komplexität adressierten Reaktionen wider. Der Anwendungsbereich umfasst wichtiger Zwischenprodukte pharmazeutische chemische Industrie sowie Entwicklung enzymatischer Techniken Prozesse. Enzyme sind ein...

Recent advances in bioeconomy allow a holistic view of existing and new process chains enable novel production routines continuously advanced by academia industry. All this progress benefits from growing number prediction tools that have found their way into the field. For example, automated genome annotations, for building model structures proteins, structural protein methods such as AlphaFold2

Abstract The asymmetric dihydroxylation of olefins is special interest due to the facile transformation chiral diol products into valuable derivatives. Rieske non‐heme iron oxygenases (ROs) represent promising biocatalysts for this reaction as they can be engineered efficiently catalyze selective mono‐ and various olefins. introduction a single point mutation improved selectivities (≥95 %) conversions (>99 towards selected alkenes. By modifying size one active site amino acid side chain,...

The rapidly growing area of asymmetric imine reduction by reductases (IREDs) has provided alternative routes to chiral amines. Here we report the expansion reaction scope IREDs showing stereoselective 2,2,2-trifluoroacetophenone. Assisted an in silico analysis energy barriers, evaluated hydrogenations carbonyls and imines while considering influence substrate reactivity on chemoselectivity this novel class reductases. We C=N as well C=O bonds catalysed members IRED enzyme family.

Abstract The direct enantioselective addition of water to unactivated alkenes could simplify the synthesis chiral alcohols and solve a long‐standing challenge in catalysis. Here we report that an engineered fatty acid hydratase can catalyze asymmetric hydration various terminal internal alkenes. In presence carboxylic decoy molecule for activation oleate from E. meningoseptica , was achieved with up 93 % conversion, excellent selectivity (>99 ee >95 regioselectivity), on preparative scale.

Promiscuous enzymes: The substrate promiscuity of squalene–hopene cyclases has been explored and applied in the enzyme-catalyzed synthesis heterocyclic terpenoids. Features this work include cyclization reactions without pyrophosphate activation, stereospecific ring closure substrates varying chain length terminal nucleophile. This provides a biocatalytic alternative to traditional chemical catalysts. As service our authors readers, journal supporting information supplied by authors. Such...

ABSTRACT Chiral amines are valuable building blocks for the production of a variety pharmaceuticals, agrochemicals and other specialty chemicals. Only recently, imine reductases (IREDs) were discovered which catalyze stereoselective reduction imines to chiral amines. Although several IREDs biochemically characterized in last few years, knowledge reaction mechanism molecular basis substrate specificity stereoselectivity is limited. To gain further insights into sequence‐function...

Abstract We have developed a scalable platform that employs electrolysis for an in vitro synthetic enzymatic cascade continuous flow reactor. Both H 2 and O were produced by transferred through gas‐permeable membrane into the system. The enabled separation of electrolyte from biocatalysts system, where served as electron mediators biocatalysts. demonstrate production methylated N‐heterocycles diamines with up to 99 % product formation well excellent regioselective labeling stable isotopes....

Iván Lavandera García, professor of organic chemistry at the University Oviedo, Spain, passed away after a long battle with cancer on February 13, 2025, age only 48. was fantastic friend, colleague, and dedicated mentor to many students, who profited from his innovative, forward-thinking, team-oriented spirit which pushed boundaries biocatalysis bioorganic chemistry. He will be remembered as one most cheerful passionate chemists, and, all, dear friend. García born in Barcelona (Spain) July...

Iván Lavandera García, professor of organic chemistry at the University Oviedo, Spain, passed away after a long battle with cancer on February 13, 2025, age only 48. was fantastic friend, colleague, and dedicated mentor to many students, who profited from his innovative, forward-thinking, team-oriented spirit which pushed boundaries biocatalysis bioorganic chemistry. He will be remembered as one most cheerful passionate chemists, and, all, dear friend. García born in Barcelona (Spain) July...