Although flow cytometry produces reliable results, the data processing from gating to fingerprinting is prone subjective bias. Here, we integrated autogating with Automated Machine Learning in enhance classification of bacterial phenotypes. We analyzed six strains prevalent soil and groundwater-Bacillus subtilis, Burkholderia thailandensis, Corynebacterium glutamicum, Escherichia coli, Pseudomonas putida, stutzeri. Using H2O-AutoML framework, applied gradient-boosting machine (GBM) models...

To find environmental variables (EVs) shaping the ecological niche of archaeal phylum Thaumarchaeota in terrestrial environments, we determined abundance various soil samples using real-time PCR targeting thaumarchaeotal 16S rRNA gene sequences. We employed our previously developed primer, THAUM-494, which had greater coverage for and lower tolerance to nonthaumarchaeotal taxa than previous Thaumarchaeota-directed primers. The relative estimates (RVs) (RTHAUM), Archaea (RARCH), Bacteria...

Drought has become a major agricultural threat leading crop yield loss. Although few species of rhizobacteria have the ability to promote plant growth under drought, drought tolerance soil microbiome and its relationship with promotion are scarcely studied. This study aimed develop novel approach for assessing in land by quantitatively measuring microbial phenotypes using stable isotopes Raman spectroscopy. spectroscopy deuterium isotope probing was used identify signatures effects from...

The bacteria strain EN12T was isolated from forest soil in the Republic of Korea. cells were Gram-negative, non-motile and rod-shaped, strictly aerobic. Phylogenetic analysis its 16S rRNA gene sequences showed that belonged to class Sphingobacteriia phylum Bacteroidetes, closest relative is Pedobacter namyangjuensis 5G38T, with a sequence similarity 95.5 %. average DNA validly described species within genus 92.5±1.3 Chemotaxonomic data including major ubiquinones (menaquinone-7), polar...

Microplastics (MPs), which result from the breakdown of plastic waste, have become ubiquitous in various environmental compartments. The “plastisphere”, referring to unique bacterial communities inhabiting debris, includes pathogens and antibiotic resistance genes. Wastewater treatment plants (WWTPs) are hotspots for plastisphere formation, but significant releases MPs still occur. This study investigates microbial on polystyrene (PS) through situ deployment across primary, secondary,...

Based on comparative phylogenetic analysis of 16S rRNA gene sequences deposited in an RDP database, we constructed a local database thaumarchaeotal and developed novel PCR primer specific for the archaeal phylum Thaumarchaeota. Among 9,727 quality-filtered (chimeral-checked, size >1.2 kb) downloaded from 1,549 were identified included our database. In study, Thaumarchaeota groups MG-I, SAGMCG-I, SCG, FSCG, RC, HWCG-III, forming monophyletic group tree. Cluster revealed 114 phylotypes The...

DNA polymerase (pol) κ efficiently catalyzes error-free translesion synthesis (TLS) opposite bulky N2-guanyl lesions induced by carcinogens such as polycyclic aromatic hydrocarbons. We investigated the biochemical effects of nine human nonsynonymous germline POLK variations on TLS properties pol κ, utilizing recombinant (residues 1-526) enzymes and templates containing an N2-CH2(9-anthracenyl)G (N2-AnthG), 8-oxo-7,8-dihydroguanine (8-oxoG), O6-methyl(Me)G, or abasic site. In steady-state...

A gram-negative, rod shaped bacterium designated as strain H2T was isolated from an artificial pond in Korea. The able to grow aerobically and anaerobically with optimal growth occurring at 30 °C pH 7.0 under aerobic conditions. Phylogenetic analysis based on 16S rRNA gene sequences showed that the belonged genus Chitinimonas of family Burkholderiaceae. similarity calculated valid species belongs ranged 93.2 % (for taiwanensis cfT) 94.4 prasina LY03T), formed a tight monophyletic group them....

BACKGROUND: Animal manures are one of the biggest sources greenhouse gases and improper manage-ment animal wastes contributes to increasing in atmosphere.Con-verting generated from energy is way contributing net-zero carbon emissions.METHODS AND RESULTS: The potential for methane production cow manure (CM) was studied by measuring yield using biochemical (BMP) test.In particular, effect co-digestion rice straw (RM) on studied.The yields CM RS were statistically similar that mono-digestion or...

The complex interplay between an animal and its surrounding environment requires constant attentive observation in natural settings. Moreover, how ecological interactions are affected by animal’s genes is difficult to ascertain outside the laboratory. Genetic studies with bacterivorous nematode Caenorhabditis elegans have elucidated numerous relationships functions, such as physiology, behaviors, lifespan. However, these use standard laboratory culture that does not reflect C. true ecology....

Industrial wastewater has attracted increasing attention in recent years because of its impact on ecosystems and human health. Whole-effluent tests are generally used to monitor toxicities unknown chemicals conventional pollutants from industrial effluent discharges. This study described identification evaluation (TIE) procedures determine the acute toxicity a nonmetallic mineral mining facility that was toxic Daphnia magna. In characterization step (TIE phase I), effects heavy metals,...

Human Y-family DNA polymerase (pol) ι is involved in translesion synthesis (TLS) and base excision repair (BER) of oxidative damage. Genetic variations may alter the function pol affect cellular susceptibility to genotoxic agents, but their effects remain unclear. We investigated impacts 10 human missense germline on by biochemical cell-based assays. Both deoxyribose phosphate (dRP) lyase activities were determined utilizing recombinant (residues 1–445) proteins. The K209Q, K228I, Q386R...

DNA polymerase (pol) η is responsible for error-free translesion synthesis (TLS) opposite ultraviolet light (UV)-induced cis-syn cyclobutane thymine dimers (CTDs) and cisplatin-induced intrastrand guanine crosslinks. POLH deficiency causes one form of the skin cancer-prone disease xeroderma pigmentosum variant (XPV) cisplatin sensitivity, but functional impacts its germline variants remain unclear. We evaluated properties eight human in silico-predicted deleterious missense variants, using...

Large amounts of organic wastes generated in agricultural environments such as crop residues and livestock manure adversely affect the environment.Anaerobic digestion can reduce amount convert them into energy at same time.Efforts are being made to further increase conversion efficiency by using co-anaerobic two or more substrates.Tomatoes, rice straw, cattle manure, feces (CF) were used substrates for anaerobic digestion.Each substrate was subjected cumulative biochemical methane production...