A5075924009- Ion Channels and Receptors
- Phytochemicals and Antioxidant Activities
- Neurobiology and Insect Physiology Research
- Mitochondrial Function and Pathology
- Calcium signaling and nucleotide metabolism
- RNA and protein synthesis mechanisms
- Postharvest Quality and Shelf Life Management
- Biochemical Analysis and Sensing Techniques
- Plant and Biological Electrophysiology Studies
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- RNA Research and Splicing
- Photoreceptor and optogenetics research
- Cannabis and Cannabinoid Research
- PARP inhibition in cancer therapy
- Bioactive Compounds and Antitumor Agents
- Herbal Medicine Research Studies
- Dermatology and Skin Diseases
- Photosynthetic Processes and Mechanisms
- Biocrusts and Microbial Ecology
- Lipid Membrane Structure and Behavior
- Pacific and Southeast Asian Studies
- Medical Practices and Rehabilitation
- Psychedelics and Drug Studies
Leipzig University
2012-2023
Max Planck Institute for Biophysical Chemistry
2008
Charité - Universitätsmedizin Berlin
2005-2008
GlaxoSmithKline (United Kingdom)
2004-2006
Osnabrück University
1998-2005
New Frontier
2004
Kiel University
1998
1. TRPM2 (melastatin-like transient receptor potential 2 channel) is a nonselective cation channel that activated under conditions of oxidative stress leading to an increase in intracellular free Ca(2+) concentration ([Ca(2+)](i)) and cell death. We investigated the role DNA repair enzyme poly(ADP-ribose) polymerase (PARP) on hydrogen peroxide (H(2)O(2))-mediated activation using tetracycline-inducible TRPM2-expressing line. 2. In whole-cell patch-clamp recordings, adenine 5'-diphosphoribose...
The preprotein translocase of the yeast mitochondrial outer membrane (TOM) consists initial import receptors Tom70 and Tom20 a approximately 400-kDa (400 K) general pore (GIP) complex that includes central receptor Tom22, channel Tom40, three small Tom proteins Tom7, Tom6, Tom5. We report GIP is highly stable with an unusual resistance to urea alkaline pH. Under mild conditions for lysis, Tom20, but not Tom70, quantitatively associated complex, forming 500K 600K TOM complex. A preprotein,...
Canonical transient receptor potential channel 5 (TRPC5) is a nonselective, Ca<sup>2+</sup>-permeable cation that belongs to the large family of channels. It predominantly found in central nervous system with high expression density hippocampus, amygdala, and frontal cortex. Several studies confirm TRPC5 channels are implicated regulation neurite length growth cone morphology. We identified clemizole as novel inhibitor Clemizole efficiently blocks currents Ca<sup>2+</sup> entry low...
The pea chloroplastic outer envelope protein OEP24 can function as a general solute channel. is present in chloroplasts, etioplasts, and non-green root plastids. heterologously expressed forms voltage-dependent, high-conductance (Lambda = 1.3 nS 1 M KCl), slightly cation-selective ion channel reconstituted proteoliposomes. highest open probability (P approximately 0. 8) at 0 mV, which consistent with the absence of transmembrane potential across envelope. channels allow flux triosephosphate,...
TRPA1, a poorly selective Ca2+-permeable cation channel, is expressed in peripheral sensory neurons, where it considered to contribute variety of processes such as the detection painful stimuli. Furthermore, TRPA1 was also identified hair cells inner ear, but its involvement sensing mechanical forces still being controversially discussed. Amphipathic molecules trinitrophenol and chlorpromazine have been shown provide useful tools study mechanosensitive channels. Depending on their charge,...
SNARE-mediated exocytosis is a multistage process central to synaptic transmission and hormone release. Complexins (CPXs) are small proteins that bind very rapidly with high affinity the SNARE core complex, where they have been proposed recently inhibit by clamping complex inhibiting membrane fusion. However, several other studies also suggest CPXs positive regulators of neurotransmitter Thus, whether or negative not known, much less stage in vesicle life cycle at which function. Here, we...
Phospholipids occurring in cell membranes and lipoproteins are converted into oxidized phospholipids (OxPL) by oxidative stress promoting atherosclerotic plaque formation. Here, OxPL were characterized as novel targets acute chronic inflammatory pain. Oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (OxPAPC) its derivatives identified inflamed tissue mass spectrometry binding assays. They elicited calcium influx, hyperalgesia induced pro-nociceptive peptide release. Genetic,...
We report the detailed expression profile of TRPM2 mRNA within human central nervous system (CNS) and demonstrate increased at 1 4 weeks following ischemic injury in rat transient middle cerebral artery occlusion (tMCAO) stroke model. Microglial cells play a key role pathology produced CNS possess TRPM2, which may contribute to stroke-related pathological responses. show that is present C13 microglial cell line reduced by antisense treatment. Activation interleukin-1β leads fivefold increase...
The transient receptor potential channel C5 (TRPC5) is a Ca(2+)-permeable cation channel, which predominantly expressed in the brain. TRPC5 activated PLC-dependent manner by, as yet, unidentified endogenous messengers. Recently, modulators of TRPC5, like Ca(2+), pH and phospholipids, have been identified. However, role vivo only poorly understood. Novel specific might help to elucidate its function.Novel were identified compound screening approved drugs natural compounds. potency selectivity...
In macrophages, transient receptor potential vanilloid 2 (TRPV2) channel contributes to various cellular processes such as cytokine production, differentiation, phagocytosis and migration. Due a lack of selective pharmacological tools, its function in immunological is not well understood the identification novel TRPV2 modulators highly desirable.Novel were identified by screening compound library using Ca2+ influx assays with human embryonic kidney 293 (HEK293) cells heterologously...
The human hyperpolarization‐activated cyclic nucleotide‐gated 1 (hHCN1) subunit was heterologously expressed in mammalian cell lines (CV‐1 and CHO) its properties investigated using whole‐cell patch‐clamp recordings. Activation of this recombinant channel, by membrane hyperpolarization, generated a slowly activating, noninactivating inward current. pharmacological hHCN1‐mediated currents resembled those native ( I h ), that is, blockade Cs + (99% at 5 m M ZD 7288 (98% 100 μ ) zatebradine...
Apomorphine is a non-narcotic derivative of morphine, which acts as dopamine agonist and clinically used to treat "off-states" in patients suffering from Parkinson's disease. Adverse effects apomorphine treatment include severe emesis nausea, ulceration pain at the injection site. We wanted test whether sensory transient receptor potential (TRP) channels are molecular target for apomorphine. Here, we show that rTRPV1, rTRPV2, rTRPV3, mTRPV4, well hTRPM8, rTRPM3, expressed dorsal root...
The transient receptor potential cation channel, subfamily M, members 6 and 7 (TRPM6 TRPM7) are homologous membrane proteins encompassing channel units fused to cytosolic serine/threonine-protein kinase domains. Clinical studies experiments with animal disease models suggested that selective inhibition of TRPM6 TRPM7 currents might be beneficial for subjects immune cardiovascular disorders, tumours other pathologies, but the suitable pharmacological toolkit remains underdeveloped. present...