A5017904269- Helicobacter pylori-related gastroenterology studies
- Advanced Electron Microscopy Techniques and Applications
- Galectins and Cancer Biology
- RNA and protein synthesis mechanisms
- Veterinary medicine and infectious diseases
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Electron and X-Ray Spectroscopy Techniques
- Enzyme Structure and Function
- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Hepatitis B Virus Studies
- Glycosylation and Glycoproteins Research
- Diabetic Foot Ulcer Assessment and Management
- DNA Repair Mechanisms
- Advanced X-ray Imaging Techniques
- Retinal Imaging and Analysis
- Microbial metabolism and enzyme function
- Cell Adhesion Molecules Research
- Skin and Cellular Biology Research
- Prostate Cancer Treatment and Research
- Neurogenetic and Muscular Disorders Research
- Freezing and Crystallization Processes
- Viral Infections and Outbreaks Research
University of Michigan
2018-2024
Michigan United
2023
Vanderbilt University
2015-2018
Higher-order structures of the microtubule (MT) cytoskeleton are comprised two architectures: bundles and asters. Although both architectures critical for cellular function, molecular pathways that drive aster formation poorly understood. Here, we study by human minus-end-directed kinesin-14 (HSET/KIFC1). We show HSET is incapable forming asters from preformed, nongrowing MTs, but rapidly forms MT in presence soluble (non-MT) tubulin. binds tubulin via its N-terminal tail domain to form...
Microvilli are actin-based protrusions found on the surface of diverse cell types, where they amplify membrane area and mediate interactions with external environment. In intestinal tract, these play central roles in nutrient absorption host defense therefore essential for maintaining homeostasis. However, mechanisms controlling microvillar assembly remain poorly understood. Here we report that multifunctional actin regulator cordon bleu (COBL) promotes growth brush border (BB) microvilli....
Recent outbreaks of emerging infectious diseases, such as Ebola virus disease (EVD), highlight the urgent need to develop effective countermeasures, including prophylactic vaccines. Subunit proteins derived from pathogens provide a safe source antigens for vaccination, but they are often limited by their low immunogenicity. We have developed multilamellar vaccine particle (MVP) system composed lipid–hyaluronic acid multi-cross-linked hybrid nanoparticles vaccination with protein and...
Structural studies of membrane proteins, especially small are associated with well-known experimental challenges. Complexation monoclonal antibody fragments is a common strategy to augment such proteins; however, generating that specifically bind target protein not trivial. Here we identify helical epitope, from the membrane-proximal external region (MPER) gp41-transmembrane subunit HIV envelope protein, recognized by several well-characterized antibodies and can be fused as contiguous...
Cryogenic electron microscopy (cryo-EM) has become a widely used tool for determining the protein structure. Despite recent technical advances, sample preparation remains major bottleneck several reasons, including denaturation at air-water interface, presence of preferred orientations, nonuniform ice layers, etc. Graphene, two-dimensional allotrope carbon consisting single atomic layer, recently gained attention as near-ideal support film cryo-EM that can overcome these challenges because...
Abstract Nuclear exclusion and cytoplasmic accumulation of the RNA-binding protein TDP43 are characteristic amyotrophic lateral sclerosis (ALS) frontotemporal lobar degeneration (FTLD). Despite this, origin ultrastructure cytosolic deposits remain unknown. Accumulating evidence suggests that abnormal RNA homeostasis can drive pathological mislocalization, enhancing misprocessing due to loss nuclear engendering a cycle ends in cell death. Here, we show adding small monovalent oligonucleotides...
Helicobacter pylori VacA is a secreted toxin that assembles into water-soluble oligomeric structures and forms anion-selective membrane channels. Acidification of purified enhances its activity in cell culture assays.
Abstract Higher-order structures of the microtubule (MT) cytoskeleton are comprised two architectures: bundles and asters. Although both architectures critical for cellular function, molecular pathways that drive aster formation poorly understood. Here, we study by human minus-end directed kinesin-14 (HSET/ KIFC1 ). We show HSET is incapable forming asters from pre-formed, non-growing MTs, but rapidly forms MT in presence soluble tubulin. binds (non-polymer) tubulin via its N-terminal tail...
Journal Article Image Processing Pipeline for In Situ Structural Characterization of Filaments Get access Matthew L Chang, Chang Life Sciences Institute, University Michigan, Ann Arbor, United StatesDepartment Biomedical Engineering, States Search other works by this author on: Oxford Academic Google Scholar Amanda Erwin, Erwin StatesDepartments Cell and Developmental Biology Biological Chemistry, Michigan Medical School, Shyamal Mosalaganti Biophysics, Corresponding author:...
Journal Article Structural Analysis of Helicobacter pylori VacA Reveals Insights into Oligomerization Get access Amanda L Erwin, Erwin Life Sciences Institute, University Michigan, Ann Arbor, MI, USADepartment Cell and Developmental Biology, USA Search for other works by this author on: Oxford Academic Google Scholar Min Su, Su Anne M Campbell, Campbell Department Medicine, Vanderbilt School Nashville, TN, David Akey, Akey D Borden Lacy, Lacy Pathology, Microbiology, Immunology, Timothy...
Journal Article Structural Analysis of Helicobacter pylori VacA's Channel in Membrane Get access Sarah M Connolly, Connolly Life Sciences Institute, University Michigan, Ann Arbor, MI, USACellular and Molecular Biology Program, USA Search for other works by this author on: Oxford Academic Google Scholar Amanda L Erwin, Erwin Megan Sabb, Sabb Georgia Caso, Caso Department Medicine, Vanderbilt School Nashville, TN, Timothy Cover, Cover USADepartment Pathology, Microbiology, Immunology,...
Abstract Cryogenic electron microscopy (cryo-EM) has become a widely used tool for determining protein structure. Despite recent technology advances, sample preparation remains major bottleneck several reasons, including denaturation at the air/water interface, presence of preferred orientations, nonuniform ice layers, etc. Graphene, two-dimensional allotrope carbon consisting single atomic layer, recently gained attention as near-ideal support film cryo-EM that can overcome these challenges...