A5039540619- PARP inhibition in cancer therapy
- DNA Repair Mechanisms
- Natural Compound Pharmacology Studies
- Nitric Oxide and Endothelin Effects
- Cytokine Signaling Pathways and Interactions
- Tea Polyphenols and Effects
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Liver physiology and pathology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Mitochondrial Function and Pathology
- Organ Transplantation Techniques and Outcomes
- Genomics, phytochemicals, and oxidative stress
- Bioactive Compounds and Antitumor Agents
- Toxin Mechanisms and Immunotoxins
- Immune Cell Function and Interaction
- Pancreatic function and diabetes
- Neuroscience and Neuropharmacology Research
- NF-κB Signaling Pathways
- Epigenetics and DNA Methylation
- Pharmacological Effects of Natural Compounds
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
University of Verona
2014-2024
Università Cattolica del Sacro Cuore
2006
University of Naples Federico II
2006
University of Messina
2006
Centro Neurolesi Bonino Pulejo
2006
Regione del Veneto
2002
University of Perugia
1993
Université Laval
1992
Istituto di Chimica Biomolecolare
1990
National Cancer Centre Japan
1988
The reactive nitrogen species, nitric oxide (NO), plays an important role in the pathogenesis of neurodegenerative diseases. suppression NO production may be fundamental for survival neurons. Here, we report that pretreatment human ramified microglial cells with nearly physiological levels exogenous prevents lipopolysaccharide (LPS)/tumor necrosis factor α (TNFα)-inducible synthesis, because by affecting NF-κB activation it inhibits inducible Ca2+-independent synthase isoform (iNOS) mRNA...
This study explores the impact of antiretroviral administration on expression human endogenous retroviruses (HERVs), cell growth, and invasive capability melanoma lines in culture. We investigated three antiretrovirals-lamivudine, doravirine, cabotegravir-in A375, FO-1, SK-Mel-28, BRAF-mutated, MeWo, P53-mutated, lines. The findings indicate a general these drugs to downregulate HERV-K Pol Env genes hinder viability, mobility, colony formation capacity cells. also demonstrate selectivity...
We have previously demonstrated that STAT-1 plays a critical role in promoting apoptotic cell death cardiac myocytes following ischemia/reperfusion (I/R) injury. Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, has recently been reported to inhibit activity noncardiac cells. In present study, we assessed protective effects EGCG and tea extract (GTE) infusion on both cultures isolated rat heart. reduced phosphorylation protected against I/R-induced death. Moreover,...
Abstract Glutamate neurotoxicity is correlated with an increase of cytosolic free Ca 2+ . In some cell systems, activation dependent endonucleases or formation radicals can damage DNA and activate the chromatin bound enzyme poly(ADP‐ribose) polymerase (pADPRP). We have investigated whether pADPRP may be involved in glutamate vitro. Cerebellar granule cells at 12 days culture when treated a toxic dose (100 μM) showed rapid transient poly ADP‐ribose immunoreactivity. Cellular immunostaining...
A significant epidemiological association between obesity and pancreatic ductal adenocarcinoma (PDAC) has previously been described, as well a correlation the degree of steatosis, PDAC risk prognosis. The underlying mechanisms are still not completely known.After co-culture 3T3-L1 adipocytes MiaPaCa2 with an in vitro transwell system we observed appearance fibroblast-like cells, along decrease number size remaining adipocytes. RT-PCR analyses showed gene expression typical markers mature...
The thyroid hormone (T3) affects cell growth, differentiation, and regulates metabolic functions via its interaction with the nuclear receptors (TRs). mechanism by which TRs mediate growth is unknown. To investigate mechanisms responsible for mitogenic effect of T3, we have determined changes in activation transcription factors, mRNA levels immediate early genes, proteins involved progression from G1 to S phase cycle. We show that hepatocyte proliferation induced a single administration T3...
Green tea has been reported to show anti-inflammatory properties because of its inhibitory effects on the expression several pro-inflammatory genes. Because inducible nitricoxide synthase (iNOS) plays an important role in chronic inflammatory diseases, we have focused our attention regulation iNOS by green two different human epithelial cell lines, alveolar A549/8 and colon DLD-1 cells. With use electrophoretic mobility shift assays, found a tea-mediated down-regulation DNA binding activity...
Abstract The basal expression of the proto‐oncogene c‐ fos was studied by Northern blot analysis in different regions rat brain during 24 h. A striking spontaneous oscillation mRNA detected animals kept conditions with a 12 h light/12 dark cycle. In these just detectable rest hours (morning through afternoon), and high activity (night). periodicity this persisted became free‐running when were exposed for 6 consecutive days to constant light or darkness. It thus demonstrated that fluctuation...
Multiple-organ failure (MOF) is defined as the progressive deterioration in function which occurs several organs or systems patients with septic shock, multiple trauma, severe burns, pancreatitis. In present study, we have investigated effects of Green Tea extract (GTE) on development general inflammation caused by zymosan (500 mg/kg, administered i.p. a suspension saline) mice. Organ and systemic mice was assessed 18 hours after administration and/or GTE monitored for 12 days (for loss body...
Flavonoids exhibit a variety of beneficial effects in cardiovascular diseases. Although their therapeutic properties have been attributed mainly to antioxidant action, they additional protective mechanisms such as inhibition signal transducer and activator transcription 1 (STAT1) activation. Here, we investigated the cardioprotective strong flavonoids quercetin, myricetin delphinidin. all them protect heart from ischemia/reperfusion‐injury, delphinidin exert more pronounced action than...
Inflammatory bowel disease (IBD) is characterised by oxidative and nitrosative stress, leukocyte infiltration, up-regulation of intercellular adhesion molecule 1 (ICAM-1) expression in the colon. The aim present study was to examine effects green tea extract rats subjected experimental colitis induced intracolonic instillation dinitrobenzene sulphonic acid (DNBS). At 4 days after DNBS administration were sacrificed. Treatment with significantly attenuated diarrhoea loss body weight. This...
St. John9s wort (SJW) has been described to show anti-inflammatory properties due its inhibitory effects on the expression of pro-inflammatory genes like cyclooxygenase-2, interleukin-6, and inducible nitric-oxide synthase (iNOS). Since iNOS plays a critical role in chronic inflammatory diseases, we have focused our attention regulation by SJW two different human epithelial cell lines, alveolar A549/8 colon DLD-1 cells. extract concentration dependently inhibited evaluated measuring amounts...
Abstract We previously observed that Gadd45β/MyD118, a member of the Gadd45 family inducible factors, showed strongest immediate-early induction common to two distinctive proliferation responses liver: (1) regeneration induced by surgical partial hepatectomy and (2) hyperplasia primary mitogen TCPOBOP, ligand constitutive androstane receptor (CAR). Gadd45β is known be stimulated nuclear factor (NF) κB, which activated tumor necrosis alpha (TNFα) in early response hepatectomy. therefore...