A5024374337- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- S100 Proteins and Annexins
- Pregnancy and preeclampsia studies
- Neurological Disease Mechanisms and Treatments
- Platelet Disorders and Treatments
- Bone Metabolism and Diseases
- Food Allergy and Anaphylaxis Research
- Protease and Inhibitor Mechanisms
- Blood disorders and treatments
- Psoriasis: Treatment and Pathogenesis
- Mast cells and histamine
- Vitamin D Research Studies
- Calpain Protease Function and Regulation
- Monoclonal and Polyclonal Antibodies Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammation biomarkers and pathways
- Antimicrobial Peptides and Activities
- Anesthesia and Neurotoxicity Research
- RNA regulation and disease
- Gestational Diabetes Research and Management
- Cytokine Signaling Pathways and Interactions
- Chemokine receptors and signaling
- Rheumatoid Arthritis Research and Therapies
- Receptor Mechanisms and Signaling
Nantong University
2007-2023
Soochow University
2017-2020
Shaanxi Institute of Zoology
1999
Oxymatrine (OMT) is the quinolizidine alkaloid extracted from Chinese herb Sophora flavescens Ait. that has many pharmacological effects and used for treatment of some inflammatory diseases. In this study, RAW264.7 cells THP-1 differentiated macrophages were pretreated with various concentrations OMT at 2 h prior to lipopolysaccharide (LPS) (1.0 μg/mL) different durations. We detected anti-inflammatory effect in LPS-stimulated investigated molecular mechanism. showed pretreatment...
Objective— Thrombophilia is a major complication in preeclampsia, disease associated with placental hypoxia and trophoblast inflammation. Preeclampsia women are known to have increased circulating microparticles that procoagulant, but the underlying mechanisms remain unclear. In this study, we sought understand mechanism connecting hypoxia, microparticles, thrombophilia. Approach Results— We analyzed protein markers on plasma from preeclampsia found were mostly endothelial cells. proteomic...
Metastasis is the main cause of death in cancer patients; there currently no effective treatment for metastasis. This primarily due to our insufficient understanding metastatic mechanisms cancer. An increasing number studies have shown that C-X-C motif chemokine Ligand 12 (CXCL12) overexpressed various tissues and organs. It a key niche factor nurtures pre-metastatic niches (tumorigenic soil) recruits tumor cells (oncogenic "seeds") these niches, thereby fostering cell aggression...
Abstract Pre-eclampsia (PE) is a chronic inflammatory disease in pregnancy, which associated with enhanced blood coagulation and high thrombotic risk. To date, the mechanisms underlying such an association are not fully understood. Previous studies reported levels of plasma deoxyribonucleic acid (DNA) PE women, but cellular source circulation DNA remains unknown. In this study, we tested hypothesis that activated neutrophils undergoing cell death, also called NETosis, may be responsible for...
Hepsin is a transmembrane serine protease implicated in many biological processes, including hepatocyte growth, urinary protein secretion, auditory nerve development, and cancer metastasis. Zymogen activation critical for hepsin function. To date, how activated regulated cells remains an enigma. In this study, we conducted site-directed mutagenesis, cell expression, plasma membrane labeling, trypsin digestion, Western blotting, flow cytometry experiments human hepatoma HepG2 cells, where was...
Membrane-bound proteases are essential for epidermal integrity. Human airway trypsin-like protease 4 (HAT-L4) is a type II transmembrane serine protease. Currently, its biochemical property, cellular distribution and physiological function remain unknown. Here we examined HAT-L4 expression in vitro vivo. In Western analysis, expressed transfected CHO cells appeared as 48-kDa protein. Flow cytometry confirmed on the cell surface with expected membrane topology. RT-PCR immunostaining...
Cerebral neuroinflammation models were established by injecting 10 μg lipopolysaccharide into the hippocampus of male Sprague-Dawley rats. The rats treated with an intraperitoneal injection 120, 90, or 60 mg/kg oxymatrine daily for three days prior to injection. Twenty-four hours after model induction, was analyzed real-time quantitative PCR, and cerebral cortex enzyme-linked immunosorbent assay western blot assay. results PCR showed that secretion mRNA expression pro-inflammatory cytokines...
Abstract Transmembrane serine proteases have been implicated in the development and progression of solid hematological cancers. Human airway trypsin‐like protease 4 (HAT‐L4) is a transmembrane expressed epithelial cells exocrine glands. In skin, HAT‐L4 important for normal epidermal barrier function. Here, we report an unexpected finding ectopic expression neutrophils monocytes from acute myeloid leukemia (AML) patients. Such was not detected bone marrow individuals or patients with chronic...
Lipopolysaccharide stimulates Toll-like receptor 4 on immune cells to produce mediators. is also expressed by non-immune cells, which can be stimulated lipopolysaccharide. However, whether primary cultured hippocampal neurons and its specific role in lipopolysaccharide-induced neuroinflammation currently undefined. In this study, antibody blocking was used analyze the signaling pathway changes inflammation of lipopolysaccharide neurons. Immunofluorescence showed that protein mainly located...